ID A0A2I3S6N7_PANTR Unreviewed; 4374 AA.
AC A0A2I3S6N7;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=HUWE1 {ECO:0000313|Ensembl:ENSPTRP00000072651.1,
GN ECO:0000313|VGNC:VGNC:55717};
OS Pan troglodytes (Chimpanzee).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pan.
OX NCBI_TaxID=9598 {ECO:0000313|Ensembl:ENSPTRP00000072651.1, ECO:0000313|Proteomes:UP000002277};
RN [1] {ECO:0000313|Ensembl:ENSPTRP00000072651.1, ECO:0000313|Proteomes:UP000002277}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16136131; DOI=10.1038/nature04072;
RG Chimpanzee sequencing and analysis consortium;
RT "Initial sequence of the chimpanzee genome and comparison with the human
RT genome.";
RL Nature 437:69-87(2005).
RN [2] {ECO:0000313|Ensembl:ENSPTRP00000072651.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
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DR EMBL; AACZ04058781; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR Ensembl; ENSPTRT00000089340.1; ENSPTRP00000072651.1; ENSPTRG00000021930.6.
DR VGNC; VGNC:55717; HUWE1.
DR GeneTree; ENSGT00940000156319; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000002277; Chromosome X.
DR Bgee; ENSPTRG00000021930; Expressed in lymph node and 21 other cell types or tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14288; UBA_HUWE1; 1.
DR Gene3D; 3.30.720.50; -; 1.
DR Gene3D; 6.10.250.1630; -; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR041918; UBA_HUWE1.
DR InterPro; IPR004170; WWE-dom.
DR InterPro; IPR037197; WWE_dom_sf.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00627; UBA; 1.
DR Pfam; PF14377; UBM; 3.
DR Pfam; PF02825; WWE; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR SUPFAM; SSF117839; WWE domain; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50030; UBA; 1.
DR PROSITE; PS50918; WWE; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000002277};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1354..1393
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 1641..1718
FT /note="WWE"
FT /evidence="ECO:0000259|PROSITE:PS50918"
FT DOMAIN 4038..4374
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 744..797
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1016..1039
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1052..1075
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1329..1358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1434..1453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1727..1773
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2056..2103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2300..2359
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2395..2485
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2699..2836
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2853..2985
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3052..3074
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3260..3282
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3419..3439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3486..3527
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3552..3583
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3752..3773
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3794..3848
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3894..3948
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 775..794
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1329..1348
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1737..1759
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2056..2073
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2074..2098
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2300..2333
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2411..2476
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2699..2723
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2739..2779
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2818..2836
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2859..2902
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2920..2949
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3056..3074
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3260..3275
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3486..3517
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3813..3830
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3894..3911
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3929..3948
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 4341
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 4374 AA; 481654 MW; D58FD61C7A410D8D CRC64;
GEKLKETPVR FHPADCRALI DKLKVCNDEQ LLLELQQIKT WNIGKCELYH WVDLLDRFDG
ILADAGQTVE NMSWMLVCDR PEREQLKMLL LAVLNFTALL IEYSFSRHLY SSIEHLTTLL
ASSDMQVVLA VLNLLYVFSK RSNYITRLGS DKRTPLLTRL QHLAESWGGK ENGFGLAECC
RDLHMMKYPP SATTLHFEFY ADPGAEVKIE KRTTSNTLHY IHIEQLDKIS ESPSEIMESL
TKMYSIPKDK QMLLFTHIRL AHGFSNHRKR LQAVQARLHA ISILVYSNAL QESANSILYN
GLIEELVDVL QITDKQLMEI KAASLRTLTS IVHLERTPKL SSIIDCTGTA SYHGFLPVLV
RNCIQAMIDP SMDPYPHQFA TALFSFLYHL ASYDAGGEAL VSCGMMEALL KVIKFLGDEQ
DQITFVTRAV RVVDLITNLD MAAFQSHSGL SIFIYRLEHE VDLCRKECPF VIKPKIQRPN
TTQEGEEMET DMDVADVAME SSPGSSISME HRLDVELRAS GSSSSTNISS GPSPGVQCIP
QRAALLKSML NFLKKAIQDP AFSDGIRHVM DGSLPTSLKH IISNAEYYGP SLFLLATEVV
TVFVFQEPSL LSSLQDNGLT DVMLHALLIK DVPATREVLG SLPNVFSALC LNARGLQSFV
QCQPFERLFK VLLSPDYLPA MRRRRSSDPL GDTASNLGSA VDELMRHQPT LKTDATTAII
KLLEEICNLG RDPKYICQKP SIQKADGTAT APPPRSNHAA EEASSEDEEE EEVQAMQSFN
STQQNETEPN QQVVGTEERI PIPLMDYILN VMKFVESILS NNTTDDHCQE FVNQKGLLPL
VTILGLPNLP IDFPTSAACQ AVAGVCKSIL TLSHEPKVLQ EGLLQLDSIL SSLEPLHRPI
ESPGGSVLLR ELACAGNVAD ATLSAQATPL LHALTAAHAY IMMFVHTCRV GQSEIRSISV
NQWGSQLGLS VLSKLSQLYC SLVWESTVLL SLCTPNSLPS GCEFGQADMQ KLVPKDEKAG
TTQGGKRSDG EQDGAAGSMD ASTQGLLEGI GLDGDTLAPM ETDEPTASDS KGKSKITPAM
AARIKQIKPL LSASSRLGRA LAELFGLLVK LCVGSPVRQR RSHHAASTTT APTPAARSTA
SALTKLLTKG LSWQPPPYTP TPRFRLTFFI CSVGFTSPML FDERKYPYHL MLQKFLCSGG
HNALFETFNW ALSMGGKVPV SEGLEHSDLP DGTGEFLDAW LMLVEKMVNP TTVLESPHSL
PAKLPGGVQN FPQFSALRFL VVTQKAAFTC IKNLWNRKPL KVYGGRMAES MLAILCHILR
GEPVIRERLS KEKEGSRGEE DTGQEEGGSR REPQVNQQQL QQLMDMGFTR EHAMEALLNT
STMEQATEYL LTHPPPIMGG VVRDLSMSEE DQMMRAIAMS LGQDIPMDQR AESPEEVACR
KEEEERKARE KQEEEEAKCL EKFQDADPLE QDELHTFTDT MLPGCFHLLD ELPDTVYRVC
DLIMTAIKRN GADYRDMILK QVVNQVWEAA DVLIKAALPL TTSDTKTVSE WISQMATLPQ
ASNLATRILL LTLLFEELKL PCAWVVESSG ILNVLIKLLE VVQPCLQAAK EQKEVQTPKW
ITPVLLLIDF YEKTAISSKR RAQMTKYLQS NSNNWRWFDD RSGRWCSYSA SNNSTIDSAW
KSGETSVRFT AGRRRYTVQF TTMVQVNEET GNRRPVMLTL LRVPRLNKNS KNSNGQELEK
TLEESKEMDI KRKENKGNDT PLALESTNTE KETSLEETKI GEILIQGLTE DMVTVLIRAC
VSMLGVPVDP DTLHATLRLC LRLTRDHKYA MMFAELKSTR MILNLTQSSG FNGFTPLVTL
LLRHIIEDPC TLRHTMEKVV RSAATSGAGS TTSGVVSGSL GSREINYILR VLGPAACRNP
DIFTEVANCC IRIALPAPRG SGTASDDEFE NLRIKGPNAV QLVKTTPLKP SPLPVIPDTI
KEVIYDMLNA LAAYHAPEEA DKSDPKPGVM TQEVGQLLQD MGDDVYQQYR SLTRQSSDFD
TQSGFSINSQ VFAADGASTE TSASGTSQGE ASTPEESRDG KKDKEGDRAS EEGKQKGKGS
KPLMPTSTIL RLLAELVRSY VGIATLIANY SYTVGQSELI KEDCSVLAFV LDHLLPHTQN
AEDKDTPALA RLFLASLAAA GSGTDAQVAL VNEVKAALGR ALAMAESTEK HARLQAVMCI
ISTIMESCPS TSSFYSSATA KTQHNGMNNI IRLFLKKGLV NDLARVPHSL DLSSPNMANT
VNAALKPLET LSRIVNQPSS LFGSKSASSK NKSEQDAQGA SQDSSSNQQD PGEPGEAEVQ
EEDHDVTQTE VADGDIMDGE AETDSVVIAG QPEVLSSQEM QVENELEDLI DELLERDGGS
GNSTIIDSMN ILDPEDEEEH TQEEDSSGSN EDEDDSQDEE EEEEEDEEDD QEDDEGEEGD
EDDDDDGSEM ELDEDYPDMN ASPLVRFERF DREDDLIIEF DNMFSSATDI PPSPGNIPTT
HPLMVRHADH SSLTLGSGSS TTRLTQGIGR SQRTLRQLTA NTGHTIHVHY PGNRQPNPPL
ILQRLLGPSA AADILQLSSS LPLQSRGRAR LLVGNDDVHI IARSDDELLD DFFHDQSTAT
SQAGTLSSIP TALTRWTEEC KVLDAESMHD CVSVVKVSIV NHLEFLRDEE LEERREKRRK
QLAEEETKIT DKGKEDKENR DQSAQVIPKG WEDSSLTKDG TPMPDSYPTT PSSTDAATSE
SKETLGTLQS SQQQPTLPTP PALGEVPQEL QSPAGEGGSS TQLLMPVEPE ELGPTRPSGE
AETTQMELSP APTINRNVIS PCDFFLASLS PERAEDSDAL TAVSSQLEGS PMDTSSLASC
TLEEAVGDTS AAGSSEQPRA GSSTPGDAPP AVAEVQGRSD GSGESAQPPE DSSPPASSES
SSTRDSAVAI SGADSRGILE EPLPSTSSEE EDPLAGISLP EGVDPSFLAA LPDDIRREVL
QNQLGIRPPT RTAPSTNSSA PAVVGNPGVT EVSPEFLAAL PPAIQEEVLA QQRAEQQRRE
LAQNASSDTP MDPVTFIQTL PSDLRRSVLE DMEDSVLAVM PPDIAAEAQA LRREQEARQR
QLMHERLFGH SSTSALSAIL RSPAFTSRLS GNRGVQYTRL AVQRGGTFQM GGSSSHNRPS
GSNVDTLLRL RGRLLLDHEA LSCLLVLLFV DEPKLNTSRL HRVLRNLCYH AQTRHWVIRS
LLSILQRSSE SELCIETPKL TTSEEKGKKS SKSCGSSSHE NRPLDLLHKM ESKSSNQLSW
LSVSMDAALG CRTNIFQIQR SGGRKHTEKH ASGGSTVHIH PQAAPVVCRH VLDTLIQLAK
VFPSHFTQQR TKETNCESDR ERGNKACSPC SSQSSNSGIC TDFWDLLVKL DNMNVSRKGK
NSVKSVPVSA GGEGETSPYS LEASPLGQLM NMLSHPVIRR SSLLTEKLLR LLSLISIALP
ENKVSEAQAN SGSGASSTTT ATSTTSTTTT TAASTTPTPP TAPTPVTSAP ALVAATAIST
IVVAASTTVT TPTTATTTVS ISPTTKGSKS PAKVSDGGSS STDFKMVSSG LTENQLQLSV
EVLTSHSCSE EGLEDAANVL LQLSRGDSGT RDTVLKLLLN GARHLGYTLC KQIGTLLAEL
REYNLEQQRR AQCETLSPDG LPEEQPQTTK LKGKMQSRFD MAENVVIVAS QKRPLGGREL
QLPSMSMLTS KTSTQKFFLR VLQVIIQLRD DTRRANKKAK QTGRLGSSGL GSASSIQAAV
RQLEAEADAI IQMSESSQSE ASVRREESPM DVDQPSPSAQ DTQSIASDGT PQGEKEKEER
PPELPLLSEQ LSLDELWDML GECLKELEES HDQHAVLVLQ PAVEAFFLVH ATERESKPPV
RDTRESQLAH IKDEPPPLSP APLTPATPSS LDPFFSREPS SMHISSSLPP DTQKFLRFAE
THRTVLNQIL RQSTTHLADG PFAVLVDYIR VLDFDVKRKY FRQELERLDE GLRKEDMAVH
VRRDHVFEDS YRELHRKSPE EMKNRLYIVF EGEEGQDAGG LLREWYMIIS REMFNPMYAL
FRTSPGDRVT YTINPSSHCN PNHLSYFKFV GRIVAKAVYD NRLLECYFTR SFYKHILGKS
VRYTDMESED YHFYQGLVYL LENDVSTLGY DLTFSTEVQE FGVCEVRDLK PNGANILVTE
ENKKEYVHLV CQMRMTGAIR KQLAAFLEGF YEIIPKRLIS IFTEQELELL ISGLPTIDID
DLKSNTEYHK YQSNSIQIQW FWRALRSFDQ ADRAKFLQFV TGTSKVPLQG FAALEGMNGI
QKFQIHRDDR STDRLPSAHT CFNQLDLPAY ESFEKLRHML LLAIQECSEG FGLA
//
