ID A0A2I4FX72_JUGRE Unreviewed; 401 AA.
AC A0A2I4FX72;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=cysteine--tRNA ligase {ECO:0000256|ARBA:ARBA00012832};
DE EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN Name=LOC109002800 {ECO:0000313|RefSeq:XP_018836246.1,
GN ECO:0000313|RefSeq:XP_018836247.1};
OS Juglans regia (English walnut).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fagales; Juglandaceae; Juglans.
OX NCBI_TaxID=51240 {ECO:0000313|Proteomes:UP000235220, ECO:0000313|RefSeq:XP_018836246.1};
RN [1] {ECO:0000313|RefSeq:XP_018836246.1, ECO:0000313|RefSeq:XP_018836247.1}
RP IDENTIFICATION.
RC TISSUE=Leaves {ECO:0000313|RefSeq:XP_018836246.1,
RC ECO:0000313|RefSeq:XP_018836247.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594}.
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DR RefSeq; XP_018836246.1; XM_018980701.2.
DR RefSeq; XP_018836247.1; XM_018980702.2.
DR EnsemblPlants; Jr08_01250_p1; cds.Jr08_01250_p1; Jr08_01250.
DR GeneID; 109002800; -.
DR Gramene; Jr08_01250_p1; cds.Jr08_01250_p1; Jr08_01250.
DR OrthoDB; 2140072at2759; -.
DR Proteomes; UP000235220; Chromosome 8.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:InterPro.
DR Gene3D; 1.20.120.1910; Cysteine-tRNA ligase, C-terminal anti-codon recognition domain; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR015803; Cys-tRNA-ligase.
DR InterPro; IPR015273; Cys-tRNA-synt_Ia_DALR.
DR InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00435; cysS; 1.
DR PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR Pfam; PF09190; DALR_2; 1.
DR Pfam; PF01406; tRNA-synt_1e; 1.
DR PRINTS; PR00983; TRNASYNTHCYS.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|RefSeq:XP_018836246.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000235220};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 2..212
FT /note="tRNA synthetases class I catalytic"
FT /evidence="ECO:0000259|Pfam:PF01406"
FT DOMAIN 257..318
FT /note="Cysteinyl-tRNA synthetase class Ia DALR"
FT /evidence="ECO:0000259|Pfam:PF09190"
SQ SEQUENCE 401 AA; 45401 MW; 0FE1F30193D679E4 CRC64;
MLHLHCLPPS FEPRVSDHML QIIDMIKQIL DNGYAYRIDG DVYFNVYKFP EYGRLSGRKL
EDNRAGERVA VDSRKKNPAD FALWKAAKEG EPSWESPWGL GRPGWHIECS AMSAAYLGYS
FDIHGGGMDL VFPHHENEIA QSCAACKESN IRYWIHNGFV TVDSEKMSKS LGNFFTIRQV
IDLYHPLALR LFLMGTHYRS PINYSDVQLE SASDRIFYIY QTLHDCVNVL SQNDVAVQVD
LIPPDTANCI NNFQNIFVDS MSDDLHTPVV LAAISEPLKT INDLLHTRKG KKQEFRIESL
GALEKTIKNV LTVLGLVPTS YSEVLQQLRE KALKRAKLTE DQVLEKIEER TAARRNKEYE
KSDVIRKDLA AVGIALMDGA DGTTWRPAVP LALQEEKVTA T
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