ID A0A2I4GAJ4_JUGRE Unreviewed; 689 AA.
AC A0A2I4GAJ4;
DT 28-FEB-2018, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=ATP-dependent DNA helicase 2 subunit KU80 {ECO:0000256|PIRNR:PIRNR016570};
DE EC=3.6.4.12 {ECO:0000256|PIRNR:PIRNR016570};
GN Name=LOC109006155 {ECO:0000313|RefSeq:XP_018840890.1};
GN ORFNames=F2P56_016842 {ECO:0000313|EMBL:KAF5466965.1};
OS Juglans regia (English walnut).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fagales; Juglandaceae; Juglans.
OX NCBI_TaxID=51240 {ECO:0000313|Proteomes:UP000235220, ECO:0000313|RefSeq:XP_018840890.1};
RN [1] {ECO:0000313|EMBL:KAF5466965.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaves {ECO:0000313|EMBL:KAF5466965.1};
RA Martinez-Garcia P.J., Crepeau M.W., Puiu D., Gonzalez-Ibeas D., Whalen J.,
RA Stevens K., Paul R., Butterfield T., Britton M., Reagan R., Chakraborty S.,
RA Walawage S.L., Vasquez-Gross H.A., Cardeno C., Famula R., Pratt K.,
RA Kuruganti S., Aradhya M.K., Leslie C.A., Dandekar A.M., Salzberg S.L.,
RA Wegrzyn J.L., Langley C.H., Neale D.B.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAF5466965.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaves {ECO:0000313|EMBL:KAF5466965.1};
RA Marrano A., Britton M., Zimin A.V., Zaini P.A., Workman R., Puiu D.,
RA Bianco L., Allen B.J., Troggio M., Leslie C.A., Timp W., Dendekar A.,
RA Salzberg S.L., Neale D.B.;
RT "Walnut 2.0.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|RefSeq:XP_018840890.1}
RP IDENTIFICATION.
RC TISSUE=Leaves {ECO:0000313|RefSeq:XP_018840890.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Single-stranded DNA-dependent ATP-dependent helicase.
CC {ECO:0000256|PIRNR:PIRNR016570}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|PIRNR:PIRNR016570};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|PIRNR:PIRNR016570}.
CC -!- SIMILARITY: Belongs to the ku80 family. {ECO:0000256|ARBA:ARBA00007726,
CC ECO:0000256|PIRNR:PIRNR016570}.
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DR EMBL; LIHL02000007; KAF5466965.1; -; Genomic_DNA.
DR RefSeq; XP_018840890.1; XM_018985345.2.
DR STRING; 51240.A0A2I4GAJ4; -.
DR EnsemblPlants; Jr07_33320_p1; cds.Jr07_33320_p1; Jr07_33320.
DR GeneID; 109006155; -.
DR Gramene; Jr07_33320_p1; cds.Jr07_33320_p1; Jr07_33320.
DR KEGG; jre:109006155; -.
DR OrthoDB; 5884at2759; -.
DR Proteomes; UP000235220; Chromosome 7.
DR Proteomes; UP000619265; Unassembled WGS sequence.
DR GO; GO:0043564; C:Ku70:Ku80 complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0042162; F:telomeric DNA binding; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IBA:GO_Central.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR CDD; cd00873; KU80; 1.
DR Gene3D; 1.10.1600.10; -; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR Gene3D; 1.25.40.240; Ku, C-terminal domain; 1.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR024193; Ku80.
DR InterPro; IPR005160; Ku_C.
DR InterPro; IPR036494; Ku_C_sf.
DR InterPro; IPR005161; Ku_N.
DR InterPro; IPR014893; Ku_PK_bind.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12604; KU AUTOANTIGEN DNA HELICASE; 1.
DR PANTHER; PTHR12604:SF4; X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5; 1.
DR Pfam; PF02735; Ku; 1.
DR Pfam; PF03730; Ku_C; 1.
DR Pfam; PF03731; Ku_N; 1.
DR Pfam; PF08785; Ku_PK_bind; 1.
DR PIRSF; PIRSF016570; Ku80; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF101420; C-terminal domain of Ku80; 1.
DR SUPFAM; SSF100939; SPOC domain-like; 1.
DR SUPFAM; SSF53300; vWA-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR016570};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|PIRNR:PIRNR016570};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172,
KW ECO:0000256|PIRNR:PIRNR016570};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|PIRNR:PIRNR016570};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR016570};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PIRNR:PIRNR016570};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR016570};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR016570};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR016570};
KW Reference proteome {ECO:0000313|Proteomes:UP000235220}.
FT DOMAIN 278..419
FT /note="Ku"
FT /evidence="ECO:0000259|SMART:SM00559"
SQ SEQUENCE 689 AA; 77843 MW; ECD185BBFB15A725 CRC64;
MARNRETLLL LLDVGPSMHK VLPEVEKVCS MLVEKKLVYH KSDQVGIILF GTEDTKNELT
REVGGYEHVV VLQDTKVVDG DLVEALQQLP LGTFNGDFLD AIIVGMDILI KKFGETDKKK
KRLCLITNAL SPVKDPFEGT KEGQVITIAE QLVRHGMGME SIVVREGLNG SATKRIMDEN
DHLLNIFAKK TSAKLVYVES PTSLLGALRT RNISPVTIFR GDLELSSKMK IKVWVYKKTS
EEKFPTLKKY SDKAPPTDKF ATHEVKVDYE YKSVEDPSRV VPPEERIKGY RYGPQVVPIA
PSEWDAVKFK PEKSVKLLGF TNASNILRHY YMKDANVFIA DPGNTRAILA VSALARAMKE
ENKVAIVRCV WRQGQGNVIV GVLTPNVSDK ENIPDSFNFN VLPFVEDVRE FQFPSFNNFP
ASWQPNEQQQ EAADDLVKLL DLAPSGKEEA LQPEFTPNPV LQRFYHHLDL KSKHPDAAIP
PLDETLKRIT EPNPELFSLN KSAIDAFRRS FELKDNPKLK KSTRRILREK TSGSNMGKGY
ADISVQSLNS IENTSAVKIE KIGDLNPVQD FETMMSRRDG PEWIGKAIKD MKNKIFDLVE
DSYEGDNYPK ALECLVALRK GCILEQEPKQ FNDFLHHLCK FCREKNLNSF CEFLTSKELA
LISKTEAIDS EVSDDEARSF LVKSDTKIE
//
