ID A0A2I8DEX1_9BURK Unreviewed; 414 AA.
AC A0A2I8DEX1;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=carboxymethylenebutenolidase {ECO:0000256|ARBA:ARBA00044062};
DE EC=3.1.1.45 {ECO:0000256|ARBA:ARBA00044062};
GN ORFNames=C2U31_07710 {ECO:0000313|EMBL:AUT45877.1};
OS Achromobacter sp. AONIH1.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Alcaligenaceae; Achromobacter.
OX NCBI_TaxID=1758194 {ECO:0000313|EMBL:AUT45877.1, ECO:0000313|Proteomes:UP000236176};
RN [1] {ECO:0000313|Proteomes:UP000236176}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AONIH1 {ECO:0000313|Proteomes:UP000236176};
RX PubMed=29437920;
RG NISC Comparative Sequencing Program;
RA Weingarten R.A., Johnson R.C., Conlan S., Ramsburg A.M., Dekker J.P.,
RA Lau A.F., Khil P., Odom R.T., Deming C., Park M., Thomas P.J.,
RA Henderson D.K., Palmore T.N., Segre J.A., Frank K.M.;
RT "Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of
RT Bacterial Plasmids Conferring Carbapenem Resistance.";
RL MBio 9:e02011-e02017(2018).
RN [2] {ECO:0000313|Proteomes:UP000236176}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AONIH1 {ECO:0000313|Proteomes:UP000236176};
RA Segre J.A., Mullikin J.;
RL Submitted (JAN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC 2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC Evidence={ECO:0000256|ARBA:ARBA00043789};
CC -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC {ECO:0000256|ARBA:ARBA00008456}.
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DR EMBL; CP026124; AUT45877.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2I8DEX1; -.
DR KEGG; achr:C2U31_07710; -.
DR OrthoDB; 62567at2; -.
DR Proteomes; UP000236176; Chromosome.
DR GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR Gene3D; 3.10.450.50; -; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002925; Dienelactn_hydro.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR037401; SnoaL-like.
DR PANTHER; PTHR46623; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR PANTHER; PTHR46623:SF11; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR Pfam; PF01738; DLH; 1.
DR Pfam; PF12680; SnoaL_2; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF54427; NTF2-like; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000236176}.
FT DOMAIN 20..233
FT /note="Dienelactone hydrolase"
FT /evidence="ECO:0000259|Pfam:PF01738"
FT DOMAIN 252..359
FT /note="SnoaL-like"
FT /evidence="ECO:0000259|Pfam:PF12680"
SQ SEQUENCE 414 AA; 45369 MW; 2F438F3AE9EDDB96 CRC64;
MTTNVTTRRI RIAAHDGGEF EGYLAVPASG SGPGLVLCQE IFGLNGFVRQ TAERLAEEGY
VVLAPDLFWR QQPGIELTDG PDDMPRAFAL YQGFDEDLGV RDIGSAVAAL RALPELKGGV
GALGYCLGGK LAYLAACRCD VDVAVGYYGV GIENSLEEAS KLRGRLVLHI AERDGFCPPE
ARERILAALG GKPGVELYVY PGLDHAFART GGAHYDKPAA MMAHQRSMAA LQRAIGPEFD
YSHLWDKHCE YEFGTRDVAA TMATMVAEPY VNHIPTMTGG VGHKELSRFY QHHFVNSNPP
DTRLVPLSRT VGATQIVDEL LFCFTHTTEI DWMLPGVPPT GKYVEIPLVA IVKFRGDKLY
HEHIYWDQAS VLVQVGLLDP QGLPVAGAET ARKLLDETLP SNTLMARWRE SEKK
//
