ID A0A2J6K2W2_LACSA Unreviewed; 475 AA.
AC A0A2J6K2W2;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:PLY69389.1};
GN ORFNames=LSAT_8X136401 {ECO:0000313|EMBL:PLY69389.1};
OS Lactuca sativa (Garden lettuce).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC Lactucinae; Lactuca.
OX NCBI_TaxID=4236 {ECO:0000313|EMBL:PLY69389.1};
RN [1] {ECO:0000313|EMBL:PLY69389.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=28401891; DOI=10.1038/ncomms14953;
RA Reyes-Chin-Wo S., Wang Z., Yang X., Kozik A., Arikit S., Song C., Xia L.,
RA Froenicke L., Lavelle D.O., Truco M.J., Xia R., Zhu S., Xu C., Xu H.,
RA Xu X., Cox K., Korf I., Meyers B.C., Michelmore R.W.;
RT "Genome assembly with in vitro proximity ligation data and whole-genome
RT triplication in lettuce.";
RL Nat. Commun. 8:14953-14953(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC Evidence={ECO:0000256|ARBA:ARBA00029340};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}. Membrane
CC {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004167}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the long-chain O-
CC acyltransferase family. {ECO:0000256|ARBA:ARBA00024360}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PLY69389.1}.
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DR EMBL; NBSK01008932; PLY69389.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2J6K2W2; -.
DR STRING; 4236.A0A2J6K2W2; -.
DR EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_8X136401_mrna; cds-PLY69389.1; gene-LSAT_8X136401.
DR Gramene; rna-gnl|WGS:NBSK|LSAT_8X136401_mrna; cds-PLY69389.1; gene-LSAT_8X136401.
DR OrthoDB; 2957273at2759; -.
DR UniPathway; UPA00282; -.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008374; F:O-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IBA:GO_Central.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650:SF34; DIACYLGLYCEROL O-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 108..267
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 321..467
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 475 AA; 53307 MW; F02D685C728F8530 CRC64;
MELKVKVERV EDKEMSEPVS PTGQYFNSSV LSISVISILE FDNPIDDSSS LALINDVFLP
INPRFSSIMV ENKEGVKHWK RVEVKAEDHI NVPCFPEGLS PKSYDHYFND YLSKMAMDPL
PQTKPLWEIH VIKYPTSNAS GSVVFKLHHA LGDGFSLMGA LLSCLQRADN PSLPLTFPNF
QKAVKPDNEE KSLISVVPQA ISGALNTVMD FGWSVLKSSF LEDGRTPIRS GEEGVEFKPI
DIMTMTFSLD QIKQIKSRLQ VTINDVVTGM IFLGTRLYME ATNEESRNAM STALVLLNTR
SIGGYKSVDE MVQNEEAQIQ WGNQFGFLHV SLPELHEHDH SLNPLKFVQE TQNTIKRKRN
SSGVYLTGML LDSVRKYRGP EATAEYIHST LKNSSMTVSN MIGPLEKMTL SNQPIKGLYF
MVLNVPQSLT VTVMSYMDQL RIAVGTQKGF IDHVKFRTCI EKAFSMMSDA ALQSK
//
