ID A0A2J6K955_LACSA Unreviewed; 320 AA.
AC A0A2J6K955;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 08-NOV-2023, entry version 24.
DE RecName: Full=Uroporphyrinogen-III synthase {ECO:0000256|ARBA:ARBA00013109, ECO:0000256|RuleBase:RU366031};
DE EC=4.2.1.75 {ECO:0000256|ARBA:ARBA00013109, ECO:0000256|RuleBase:RU366031};
GN ORFNames=LSAT_2X45600 {ECO:0000313|EMBL:PLY71611.1};
OS Lactuca sativa (Garden lettuce).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC Lactucinae; Lactuca.
OX NCBI_TaxID=4236 {ECO:0000313|EMBL:PLY71611.1};
RN [1] {ECO:0000313|EMBL:PLY71611.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=28401891; DOI=10.1038/ncomms14953;
RA Reyes-Chin-Wo S., Wang Z., Yang X., Kozik A., Arikit S., Song C., Xia L.,
RA Froenicke L., Lavelle D.O., Truco M.J., Xia R., Zhu S., Xu C., Xu H.,
RA Xu X., Cox K., Korf I., Meyers B.C., Michelmore R.W.;
RT "Genome assembly with in vitro proximity ligation data and whole-genome
RT triplication in lettuce.";
RL Nat. Commun. 8:14953-14953(2017).
CC -!- FUNCTION: Catalyzes cyclization of the linear tetrapyrrole,
CC hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.
CC {ECO:0000256|RuleBase:RU366031}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydroxymethylbilane = H2O + uroporphyrinogen III;
CC Xref=Rhea:RHEA:18965, ChEBI:CHEBI:15377, ChEBI:CHEBI:57308,
CC ChEBI:CHEBI:57845; EC=4.2.1.75;
CC Evidence={ECO:0000256|RuleBase:RU366031};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.
CC {ECO:0000256|ARBA:ARBA00004772, ECO:0000256|RuleBase:RU366031}.
CC -!- SIMILARITY: Belongs to the uroporphyrinogen-III synthase family.
CC {ECO:0000256|ARBA:ARBA00008133, ECO:0000256|RuleBase:RU366031}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PLY71611.1}.
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DR EMBL; NBSK01008275; PLY71611.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2J6K955; -.
DR STRING; 4236.A0A2J6K955; -.
DR EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_2X45600_mrna; cds-PLY71611.1; gene-LSAT_2X45600.
DR Gramene; rna-gnl|WGS:NBSK|LSAT_2X45600_mrna; cds-PLY71611.1; gene-LSAT_2X45600.
DR UniPathway; UPA00251; UER00320.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004852; F:uroporphyrinogen-III synthase activity; IBA:GO_Central.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006780; P:uroporphyrinogen III biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.50.10090; -; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR036108; 4pyrrol_syn_uPrphyn_synt_sf.
DR InterPro; IPR003754; 4pyrrol_synth_uPrphyn_synth.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR039793; UROS/Hem4.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR38042:SF5; UROPORPHYRINOGEN-III SYNTHASE; 1.
DR PANTHER; PTHR38042; UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTIC; 1.
DR Pfam; PF02602; HEM4; 1.
DR SMART; SM00320; WD40; 1.
DR SUPFAM; SSF69618; HemD-like; 1.
DR SUPFAM; SSF50998; Quinoprotein alcohol dehydrogenase-like; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|RuleBase:RU366031}; Membrane {ECO:0000256|SAM:Phobius};
KW Porphyrin biosynthesis {ECO:0000256|RuleBase:RU366031};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW WD repeat {ECO:0000256|PROSITE-ProRule:PRU00221}.
FT TRANSMEM 96..119
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REPEAT 48..80
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT DOMAIN 212..304
FT /note="Tetrapyrrole biosynthesis uroporphyrinogen III
FT synthase"
FT /evidence="ECO:0000259|Pfam:PF02602"
SQ SEQUENCE 320 AA; 35312 MW; 59824A54D57D4418 CRC64;
MEVQFLIAAF MMIPMVLVLL QITLSQVSSY IPFTSLRLIF DNEREDLLGR HYAPVRCIEY
SYATGQLITG SWDKTLKCWD PYGGGAYERA MEFFDAMAPI IFCLHLAFVV VDFITAILIR
AIGQLLQVAD IKSLKSLGAT THLNVSEAAL VFLQAWNRLT SYSHVIYNHR AIGTPNVKVA
AVGTGTSTIF HEATPSSEQF IEVAFTPSKE VGLSKHGFHI TRLNTYTTEP VQHVEQTILQ
QALSAFVVVV ASPSAVGSWV DLLPEPHILE GSIACIGETT ALDARKLGLT NVYHPSTPSL
HGWVDTIFDA LRVHNHLSNV
//
