ID A0A2J6KBZ5_LACSA Unreviewed; 523 AA.
AC A0A2J6KBZ5;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=threonine synthase {ECO:0000256|ARBA:ARBA00013028};
DE EC=4.2.3.1 {ECO:0000256|ARBA:ARBA00013028};
GN ORFNames=LSAT_5X21680 {ECO:0000313|EMBL:PLY72605.1};
OS Lactuca sativa (Garden lettuce).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC Lactucinae; Lactuca.
OX NCBI_TaxID=4236 {ECO:0000313|EMBL:PLY72605.1};
RN [1] {ECO:0000313|EMBL:PLY72605.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=28401891; DOI=10.1038/ncomms14953;
RA Reyes-Chin-Wo S., Wang Z., Yang X., Kozik A., Arikit S., Song C., Xia L.,
RA Froenicke L., Lavelle D.O., Truco M.J., Xia R., Zhu S., Xu C., Xu H.,
RA Xu X., Cox K., Korf I., Meyers B.C., Michelmore R.W.;
RT "Genome assembly with in vitro proximity ligation data and whole-genome
RT triplication in lettuce.";
RL Nat. Commun. 8:14953-14953(2017).
CC -!- FUNCTION: Catalyzes the gamma-elimination of phosphate from L-
CC phosphohomoserine and the beta-addition of water to produce L-
CC threonine. {ECO:0000256|ARBA:ARBA00003648}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-homoserine = L-threonine + phosphate;
CC Xref=Rhea:RHEA:10840, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57590, ChEBI:CHEBI:57926; EC=4.2.3.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000051};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933,
CC ECO:0000256|PIRSR:PIRSR604450-51};
CC -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine
CC from L-aspartate: step 5/5. {ECO:0000256|ARBA:ARBA00004979}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: Belongs to the threonine synthase family.
CC {ECO:0000256|ARBA:ARBA00005517}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PLY72605.1}.
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DR EMBL; NBSK01007972; PLY72605.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2J6KBZ5; -.
DR STRING; 4236.A0A2J6KBZ5; -.
DR EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_5X21680_mrna; cds-PLY72605.1; gene-LSAT_5X21680.
DR Gramene; rna-gnl|WGS:NBSK|LSAT_5X21680_mrna; cds-PLY72605.1; gene-LSAT_5X21680.
DR OrthoDB; 544888at2759; -.
DR UniPathway; UPA00050; UER00065.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0004795; F:threonine synthase activity; IBA:GO_Central.
DR GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd01563; Thr-synth_1; 1.
DR Gene3D; 3.40.50.1100; -; 2.
DR InterPro; IPR000634; Ser/Thr_deHydtase_PyrdxlP-BS.
DR InterPro; IPR004450; Thr_synthase-like.
DR InterPro; IPR001926; TrpB-like_PALP.
DR InterPro; IPR036052; TrpB-like_PALP_sf.
DR NCBIfam; TIGR00260; thrC; 1.
DR PANTHER; PTHR10314; CYSTATHIONINE BETA-SYNTHASE; 1.
DR PANTHER; PTHR10314:SF5; THREONINE SYNTHASE 2, CHLOROPLASTIC; 1.
DR Pfam; PF00291; PALP; 1.
DR SUPFAM; SSF53686; Tryptophan synthase beta subunit-like PLP-dependent enzymes; 1.
DR PROSITE; PS00165; DEHYDRATASE_SER_THR; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898,
KW ECO:0000256|PIRSR:PIRSR604450-51};
KW Threonine biosynthesis {ECO:0000256|ARBA:ARBA00022697}.
FT DOMAIN 162..469
FT /note="Tryptophan synthase beta chain-like PALP"
FT /evidence="ECO:0000259|Pfam:PF00291"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 200
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|PIRSR:PIRSR604450-51"
SQ SEQUENCE 523 AA; 57594 MW; 665FDDA68F51ED93 CRC64;
MAASSLLRSS PFFSSPQQRN PRTINKSPRF ITIKATISSS DKSVDTTPVI KSHRRPADEN
IRQEAHRRCS TEPNNFSAKY VPFNADPGCT ESYSLDEIVY RSRSGGLLDV QHDMEALKEF
DGNYWKNLFD GRIGKTNWPY GSGVWSKKEW VLPEIDSDDI VSAFEGNSNM FWAERYGKQH
LGMNDLWVKH CGISHTGSFK DLGMTVLVSQ VNRLRKMNRP VVGVGCASTG DTSAALSAYC
ASAGIPSIVF LPANKISMAQ LVQPIANGAF VLSLDTDFDG CMQLVREVTS ELPIYLANSL
NSLRIEGQKT AAIEILQQFD WEVPDWVIVP GGNLGNIYAF YKGFQMCKEL GLVDRIPRLV
CAQAANANPL YLHYKSGWTD FSPVKAKTTF ASAIQIGDPV SIDRAVYALQ NSNGIVEEAT
EEELMDAMAQ ADSTGMFICP HTGVALTALD KLRNSGVIRP TDRTVVVSTA HGLKFTQSKI
DYHSKAIPEM ACSLANPPVN VKADFGSVMD VLKKYLLSKE SKN
//
