ID   A0A2J6KE85_LACSA        Unreviewed;      1012 AA.
AC   A0A2J6KE85;
DT   28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT   28-MAR-2018, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE            EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN   ORFNames=LSAT_6X70541 {ECO:0000313|EMBL:PLY73388.1};
OS   Lactuca sativa (Garden lettuce).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC   Lactucinae; Lactuca.
OX   NCBI_TaxID=4236 {ECO:0000313|EMBL:PLY73388.1};
RN   [1] {ECO:0000313|EMBL:PLY73388.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=28401891; DOI=10.1038/ncomms14953;
RA   Reyes-Chin-Wo S., Wang Z., Yang X., Kozik A., Arikit S., Song C., Xia L.,
RA   Froenicke L., Lavelle D.O., Truco M.J., Xia R., Zhu S., Xu C., Xu H.,
RA   Xu X., Cox K., Korf I., Meyers B.C., Michelmore R.W.;
RT   "Genome assembly with in vitro proximity ligation data and whole-genome
RT   triplication in lettuce.";
RL   Nat. Commun. 8:14953-14953(2017).
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PLY73388.1}.
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DR   EMBL; NBSK01007690; PLY73388.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2J6KE85; -.
DR   STRING; 4236.A0A2J6KE85; -.
DR   EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_6X70541_mrna; cds-PLY73388.1; gene-LSAT_6X70541.
DR   Gramene; rna-gnl|WGS:NBSK|LSAT_6X70541_mrna; cds-PLY73388.1; gene-LSAT_6X70541.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd00078; HECTc; 1.
DR   Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR   InterPro; IPR044611; E3A/B/C-like.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   PANTHER; PTHR45700; UBIQUITIN-PROTEIN LIGASE E3C; 1.
DR   PANTHER; PTHR45700:SF2; UBIQUITIN-PROTEIN LIGASE E3C; 1.
DR   Pfam; PF00632; HECT; 1.
DR   SMART; SM00119; HECTc; 1.
DR   SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR   PROSITE; PS50237; HECT; 1.
PE   4: Predicted;
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW   ECO:0000256|PROSITE-ProRule:PRU00104}.
FT   DOMAIN          687..1012
FT                   /note="HECT"
FT                   /evidence="ECO:0000259|PROSITE:PS50237"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        11..25
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        996
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ   SEQUENCE   1012 AA;  115867 MW;  99C36B8A7BF126B3 CRC64;
     MFFSGDPSNR KRVDLGGRSS KERDRKKLLE QAKLERNKRS QQRQQNSAAL TIQKCFRGRK
     VVKLERGSAR ERFYTTYGKH CERVDRQCFG PDSEFLRELL YFLSPRNASD ISTLVEACRL
     FSQFAKESGD ILNLFGGSHY SSDHALVNYR VKKLAYVCIQ AIHHNRHQLK DQLLSTSLDS
     NTPTSILLKA AALLIDPKLP WSCKIASYLL QQNIYKIFRE IVITGRESLN SESRIGNVSL
     MERVLAQVVT HTGQGHCVCP DIDLQWSFAS QILTIPFLWQ IFPHLKETYV SQGLCQYYLH
     KMATCSQNYE KVLPVDISSE FPGYACLLGN LLESVGVALS RPSCSYNMTI DFATVATLLL
     EALPPIQTSS TGNREENSIM GDDDMIIDDG SVVVPLNKEL EKQISNAIDP RFLLQLTNIL
     LGGSSSKDKI KDTEAAAVGA ACSFLHVTFT IFPLERIMTV LAYRTELVSV LWSFMKRCYE
     SQNWSSLSAM SAYLPGDAFG WLLPMAVFCP VYRHMLTIVD NEEFYEQEKP LSLKDIRLLI
     VILRQSLWQI LWLNPVAPMK FTKINLSLKK HRVEFVQYRV SVVASELLSQ LQDWNNRRQF
     AHPSNFHADG VNDHFISQAM IENTRAYDIL KQAPFLVPFT SRVKLFTSQL AAIKERLGSH
     SLFNRSTFKI RRDHILEDAF NQLSTLSEED LRGVIRVTFV NEFGVEEAGI DGGGIFKDFM
     ENITRAAFDM QYGLFKETID HLLYPNPGSG MIHEQHLQFF HFLGIILGKA MFEGILVDIP
     FATFFLSKLK QKHNYLNDLP SLDPELYRHL IFLKRYEGDL SELELYFVIV NNEFGEQTSE
     ELIPGGKNIR VVNDNVITFI HLVANHRLNT QIRLQSSHFL RGFQQLIQKD WIDMFNEHEL
     QLLISGSVDG FDVDDLRSNT NYAGGYHREH YVIDMFWEIL KNFSLENQHK FLKFVTGCSR
     GPLLGFKYLE PLFCIQRAAG SASEEALDRL PTSATCMNLL KLPPYRRFVI LF
//