ID A0A2J6KFB6_LACSA Unreviewed; 454 AA.
AC A0A2J6KFB6;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 08-NOV-2023, entry version 19.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN ORFNames=LSAT_2X40580 {ECO:0000313|EMBL:PLY73760.1};
OS Lactuca sativa (Garden lettuce).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC Lactucinae; Lactuca.
OX NCBI_TaxID=4236 {ECO:0000313|EMBL:PLY73760.1};
RN [1] {ECO:0000313|EMBL:PLY73760.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=28401891; DOI=10.1038/ncomms14953;
RA Reyes-Chin-Wo S., Wang Z., Yang X., Kozik A., Arikit S., Song C., Xia L.,
RA Froenicke L., Lavelle D.O., Truco M.J., Xia R., Zhu S., Xu C., Xu H.,
RA Xu X., Cox K., Korf I., Meyers B.C., Michelmore R.W.;
RT "Genome assembly with in vitro proximity ligation data and whole-genome
RT triplication in lettuce.";
RL Nat. Commun. 8:14953-14953(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|PIRNR:PIRNR036417}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PLY73760.1}.
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DR EMBL; NBSK01007579; PLY73760.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2J6KFB6; -.
DR STRING; 4236.A0A2J6KFB6; -.
DR EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_2X40580_mrna; cds-PLY73760.1; gene-LSAT_2X40580.
DR Gramene; rna-gnl|WGS:NBSK|LSAT_2X40580_mrna; cds-PLY73760.1; gene-LSAT_2X40580.
DR UniPathway; UPA00094; -.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR013747; ACP_syn_III_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF106; 3-KETOACYL-COA SYNTHASE 19; 1.
DR Pfam; PF08541; ACP_syn_III_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417}.
FT DOMAIN 29..306
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 318..400
FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08541"
FT ACT_SITE 168
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 247
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 321
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 325
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 354
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 358
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 454 AA; 51563 MW; 86473F7C48094A04 CRC64;
MELLATSMIL LTISYVTFWL TKLVLGTGKT CYMIDYQCYK GKKEMKLDTE QCAKIVWRNK
NLRVEDYRFL LKTMVNSGIG EETYGPKSII LGEEEHPTLV ASLSELDDVF YDTLDSIFSR
SNISPSQVDI LVVTVSLISP VPSLASRIIN HYNMRSDIKA FNLSGMGCSA SLTGIDLVQH
LFKTHKNKIA IVVSTESMSS HWYCGREGSM MLSNVLFRVG GCSMLLTNDR DRKKHAIMKL
KCMVRSHFAS DDEAYNCCMQ IEDDEGYEGF RLRKTLPIVA GRALMKNLRV LLPKVLPLWE
IIRYACLKSG SKINLKTGIE HFCIHPGGRA VIDEVGINLG LSDYDLEPSR MTLHRFGNTS
SGGLWYVLGY MEAKKRLKKG DRILMISLGA GFKSNSCVWE VTRDLDRTNV WKDMIEKYPI
KQTTNPYLEK YAWINDEDMD FVTREDADKI LGFA
//
