ID A0A2J6MJY3_LACSA Unreviewed; 538 AA.
AC A0A2J6MJY3;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=carotenoid 9,10-dioxygenase {ECO:0000256|ARBA:ARBA00039084};
DE EC=1.14.99.n4 {ECO:0000256|ARBA:ARBA00039084};
GN ORFNames=LSAT_4X31520 {ECO:0000313|EMBL:PLY99876.1};
OS Lactuca sativa (Garden lettuce).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC Lactucinae; Lactuca.
OX NCBI_TaxID=4236 {ECO:0000313|EMBL:PLY99876.1};
RN [1] {ECO:0000313|EMBL:PLY99876.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=28401891; DOI=10.1038/ncomms14953;
RA Reyes-Chin-Wo S., Wang Z., Yang X., Kozik A., Arikit S., Song C., Xia L.,
RA Froenicke L., Lavelle D.O., Truco M.J., Xia R., Zhu S., Xu C., Xu H.,
RA Xu X., Cox K., Korf I., Meyers B.C., Michelmore R.W.;
RT "Genome assembly with in vitro proximity ligation data and whole-genome
RT triplication in lettuce.";
RL Nat. Commun. 8:14953-14953(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-zeaxanthin + 2 O2 = 2 (3R)-hydroxy-beta-ionone +
CC 4,9-dimethyldodeca-2,4,6,8,10-pentaenedial; Xref=Rhea:RHEA:26393,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:27547, ChEBI:CHEBI:53171,
CC ChEBI:CHEBI:53173; EC=1.14.99.n4;
CC Evidence={ECO:0000256|ARBA:ARBA00036367};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PLY99876.1}.
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DR EMBL; NBSK01000006; PLY99876.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2J6MJY3; -.
DR STRING; 4236.A0A2J6MJY3; -.
DR EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_4X31520_mrna; cds-PLY99876.1; gene-LSAT_4X31520.
DR Gramene; rna-gnl|WGS:NBSK|LSAT_4X31520_mrna; cds-PLY99876.1; gene-LSAT_4X31520.
DR GO; GO:0009570; C:chloroplast stroma; IBA:GO_Central.
DR GO; GO:0010436; F:carotenoid dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016121; P:carotene catabolic process; IBA:GO_Central.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR PANTHER; PTHR10543:SF89; CAROTENOID 9,10(9',10')-CLEAVAGE DIOXYGENASE 1; 1.
DR Pfam; PF03055; RPE65; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT BINDING 219
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 267
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 333
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 523
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 538 AA; 60534 MW; 1FBDD8190538CCB6 CRC64;
MEAEIPGGPV VVTPKPKKGF SSKAVDWLEK LIVKLMYDSS QPHHWLFGNF APVDETPPCK
DLPVIGHLPE CLNGEFVRVG PNPKFAPVAG YHWFDGDGMI HGLRIKDGKA TYLSRYVKTS
RLEQEEYFGG AKFMKIGDLK GLFGLLMVNM QMLRTKLKVL DLSFGQGTGN TALIYHHKKL
LALSEGDKPY AIKVMEDGDL ETLGLMDYDK RLSHTFTAHP KIDPVTGEMF TFGYTQSPPY
VTYRVISKDG VMNDPVPITI AEPVMMHDFA ITENYAIFMD LPLYFRPKEM VKGNKLIFTF
DSTKKARFGI LPRYAKNEVQ IKWFELPNCF IFHNANAWEE GDEVVLITCR LQNPNLDMVG
GGVKEKLDNF ANELYEMRFN METGVASQKK LSESAVDFPR VNECYTGRKQ RYVYGTRLDS
IAKVTGIVKF DLHAEPETGK TKLEIGGNIP GIYDLGPGRY GSEAIFVPKK PGFDSEEDDG
YLIFFTHDET TGKSTVTVID AKTMSPDPVA VVPLPHRVPY GFHAFFVTEE QIQEQANL
//
