ID A0A2K0X2C6_9BIFI Unreviewed; 697 AA.
AC A0A2K0X2C6;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=BFS08_04860 {ECO:0000313|EMBL:PNP88682.1};
OS Gardnerella sp. KA00735.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Gardnerella.
OX NCBI_TaxID=1973156 {ECO:0000313|EMBL:PNP88682.1, ECO:0000313|Proteomes:UP000236541};
RN [1] {ECO:0000313|Proteomes:UP000236541}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KA00735 {ECO:0000313|Proteomes:UP000236541};
RA Bumgarner R.E., Fredricks D.N., Srinivasan S.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PNP88682.1}.
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DR EMBL; NBMV01000002; PNP88682.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K0X2C6; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000236541; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.10.1660.10; -; 1.
DR Gene3D; 3.90.220.20; DNA methylase specificity domains; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR009061; DNA-bd_dom_put_sf.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR041657; HTH_17.
DR InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR025931; TaqI_C.
DR PANTHER; PTHR33841:SF1; ADENINE-SPECIFIC METHYLTRANSFERASE PGLX; 1.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR Pfam; PF12728; HTH_17; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF12950; TaqI_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF116734; DNA methylase specificity domain; 1.
DR SUPFAM; SSF46955; Putative DNA-binding domain; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 24..70
FT /note="Helix-turn-helix"
FT /evidence="ECO:0000259|Pfam:PF12728"
FT DOMAIN 230..463
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT DOMAIN 519..630
FT /note="TaqI-like C-terminal specificity"
FT /evidence="ECO:0000259|Pfam:PF12950"
SQ SEQUENCE 697 AA; 79306 MW; 1E9A36A31E0CA01B CRC64;
MRGLNTFNSE QNLTLDLLET KKTLDVNETA KKLSVSTATI RNWVKLGRIK KINTSGKKLL
FEEHEVEKLL SDIITGKSDK LTKRRNKKAI KGLNLYADYI EDDGKNILIG SELVKKDSAL
YTSIHNTLHT DTYPNQDQYP DPYPEKNIRI LIATLALKLY LQSAKLPYIS LQVFIAQNKN
DCISKLLREL LHNIDISQLG TYQNVPTPTF IPYQDFLGFI YISVSSISYR KSTGSYFTPI
NIVNKLLDSV KASTQFNNGN IIDLCCGTGN FLLASLKRGA SIERLYGNDI DEISVSIAKL
NLCINGINNI NTLNEHIRKV DSLLNRDTRK YDLVIGNPPW GYVYSETEIN KLLNSYKTAS
KKGSESYDLF MELSLNIINQ NGLIAYVLPE AILNVKAHQK IRCFIKNTSS IKFVSYIGNP
FTGVACPCIL IVLKKDGEGS TIGCNIETNN LRFTINKNRD LSEEYWNFDL SDEDVDCLEK
LTNVKNGFTL KNQADFALGI VTGANKKYIS NTKNPQNEMI LKGSNLHKYY FDNSNSYIHF
VPNTFQQVAP IEMYRAKEKL IYRFICEAPV FSYDANQTLS LNSANILIPH VTGISMKYIL
AILNSRTITY WCRKKYNSVK LLRSHIEEIP FPTPSSEEQN TIVKLVDNLL SLENHCTLDK
TTLYNEIEEH VMRLYNLNNS EKEIINSFSS QFDSFLK
//
