ID   A0A2K0X4S3_9BIFI        Unreviewed;       461 AA.
AC   A0A2K0X4S3;
DT   28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT   28-MAR-2018, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   SubName: Full=Hydroxymethylglutaryl-CoA synthase {ECO:0000313|EMBL:PNP89526.1};
GN   ORFNames=BFS08_00695 {ECO:0000313|EMBL:PNP89526.1};
OS   Gardnerella sp. KA00735.
OC   Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC   Bifidobacteriaceae; Gardnerella.
OX   NCBI_TaxID=1973156 {ECO:0000313|EMBL:PNP89526.1, ECO:0000313|Proteomes:UP000236541};
RN   [1] {ECO:0000313|Proteomes:UP000236541}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KA00735 {ECO:0000313|Proteomes:UP000236541};
RA   Bumgarner R.E., Fredricks D.N., Srinivasan S.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PNP89526.1}.
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DR   EMBL; NBMV01000001; PNP89526.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2K0X4S3; -.
DR   OrthoDB; 9769523at2; -.
DR   Proteomes; UP000236541; Unassembled WGS sequence.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR   CDD; cd00827; init_cond_enzymes; 1.
DR   Gene3D; 3.40.47.10; -; 1.
DR   InterPro; IPR013528; HMG_CoA_synth_N.
DR   InterPro; IPR011554; HMG_CoA_synthase_prok.
DR   InterPro; IPR016039; Thiolase-like.
DR   NCBIfam; TIGR01835; HMG-CoA-S_prok; 1.
DR   PANTHER; PTHR43323; 3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE; 1.
DR   PANTHER; PTHR43323:SF2; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR   Pfam; PF01154; HMG_CoA_synt_N; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 3.
PE   4: Predicted;
FT   DOMAIN          29..192
FT                   /note="Hydroxymethylglutaryl-coenzyme A synthase N-
FT                   terminal"
FT                   /evidence="ECO:0000259|Pfam:PF01154"
FT   ACT_SITE        106
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT   ACT_SITE        138
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT   ACT_SITE        260
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT   BINDING         56
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT   BINDING         170
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT   BINDING         269
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT   BINDING         305
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
SQ   SEQUENCE   461 AA;  51114 MW;  BF8F5121676BB945 CRC64;
     MTFSLKNIES NHAKQLFKDN ENTSVESNPV GIDRISFTTP HYYVDLTDLA KAREIDPNKF
     TIGIGQSEQS VPPNNQDIVT LGAQAALPLL KYVKKDKLKM LIVGTESGVD ASKSSALYIH
     NLLKLNSCAR CIEIKEACYG GTAALMTACD YVRVHKDSQV LVIAADIARY GLNTPGEVTQ
     GAGAVAMLIS ANPHVLKIND DSVVKSCDIQ DFWRPVYQDT ALARGKFSSE KYIEFFLDVW
     QKYSEQNSCD LNDFAALCFH LPYTKMGLKA LRAAMDSSEN LSETSRVRLL EKYETSTKYS
     RRIGNIYTGS LYLGLLSLLE LDGNTEIANP EIVNPGAANP AATSIQTNEI QTSTIQTNSS
     CENQKFSSLK EGDKIGLFSY GSGAIGEFFT GTLMKGFKQA LFASEHIEML NSRVKLTVSE
     YEKMFNSVAK YCPEDFVSDS SHRERTQFFL NSISSQERNY I
//