ID A0A2K1JEJ8_PHYPA Unreviewed; 1026 AA.
AC A0A2K1JEJ8;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN ORFNames=PHYPA_020224 {ECO:0000313|EMBL:PNR39944.1};
OS Physcomitrium patens (Spreading-leaved earth moss) (Physcomitrella patens).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Bryophyta;
OC Bryophytina; Bryopsida; Funariidae; Funariales; Funariaceae; Physcomitrium.
OX NCBI_TaxID=3218 {ECO:0000313|EMBL:PNR39944.1};
RN [1] {ECO:0000313|EMBL:PNR39944.1, ECO:0000313|Proteomes:UP000006727}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Gransden 2004 {ECO:0000313|EnsemblPlants:Pp3c15_25560V3.1,
RC ECO:0000313|Proteomes:UP000006727};
RX PubMed=18079367; DOI=10.1126/science.1150646;
RA Rensing S.A., Lang D., Zimmer A.D., Terry A., Salamov A., Shapiro H.,
RA Nishiyama T., Perroud P.-F., Lindquist E.A., Kamisugi Y., Tanahashi T.,
RA Sakakibara K., Fujita T., Oishi K., Shin-I T., Kuroki Y., Toyoda A.,
RA Suzuki Y., Hashimoto S.-I., Yamaguchi K., Sugano S., Kohara Y.,
RA Fujiyama A., Anterola A., Aoki S., Ashton N., Barbazuk W.B., Barker E.,
RA Bennetzen J.L., Blankenship R., Cho S.H., Dutcher S.K., Estelle M.,
RA Fawcett J.A., Gundlach H., Hanada K., Heyl A., Hicks K.A., Hughes J.,
RA Lohr M., Mayer K., Melkozernov A., Murata T., Nelson D.R., Pils B.,
RA Prigge M., Reiss B., Renner T., Rombauts S., Rushton P.J., Sanderfoot A.,
RA Schween G., Shiu S.-H., Stueber K., Theodoulou F.L., Tu H., Van de Peer Y.,
RA Verrier P.J., Waters E., Wood A., Yang L., Cove D., Cuming A.C., Hasebe M.,
RA Lucas S., Mishler B.D., Reski R., Grigoriev I.V., Quatrano R.S.,
RA Boore J.L.;
RT "The Physcomitrella genome reveals evolutionary insights into the conquest
RT of land by plants.";
RL Science 319:64-69(2008).
RN [2] {ECO:0000313|EMBL:PNR39944.1, ECO:0000313|Proteomes:UP000006727}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Gransden 2004 {ECO:0000313|EnsemblPlants:Pp3c15_25560V3.1,
RC ECO:0000313|Proteomes:UP000006727};
RX PubMed=29237241; DOI=10.1111/tpj.13801;
RA Lang D., Ullrich K.K., Murat F., Fuchs J., Jenkins J., Haas F.B.,
RA Piednoel M., Gundlach H., Van Bel M., Meyberg R., Vives C., Morata J.,
RA Symeonidi A., Hiss M., Muchero W., Kamisugi Y., Saleh O., Blanc G.,
RA Decker E.L., van Gessel N., Grimwood J., Hayes R.D., Graham S.W.,
RA Gunter L.E., McDaniel S.F., Hoernstein S.N.W., Larsson A., Li F.W.,
RA Perroud P.F., Phillips J., Ranjan P., Rokshar D.S., Rothfels C.J.,
RA Schneider L., Shu S., Stevenson D.W., Thummler F., Tillich M.,
RA Villarreal Aguilar J.C., Widiez T., Wong G.K., Wymore A., Zhang Y.,
RA Zimmer A.D., Quatrano R.S., Mayer K.F.X., Goodstein D., Casacuberta J.M.,
RA Vandepoele K., Reski R., Cuming A.C., Tuskan G.A., Maumus F., Salse J.,
RA Schmutz J., Rensing S.A.;
RT "The Physcomitrella patens chromosome-scale assembly reveals moss genome
RT structure and evolution.";
RL Plant J. 93:515-533(2018).
RN [3] {ECO:0000313|EnsemblPlants:Pp3c15_25560V3.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (DEC-2020) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
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DR EMBL; ABEU02000015; PNR39944.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K1JEJ8; -.
DR STRING; 3218.A0A2K1JEJ8; -.
DR PaxDb; 3218-PP1S253_16V6-1; -.
DR EnsemblPlants; Pp3c15_25560V3.1; Pp3c15_25560V3.1; Pp3c15_25560.
DR EnsemblPlants; Pp3c15_25560V3.2; Pp3c15_25560V3.2; Pp3c15_25560.
DR Gramene; Pp3c15_25560V3.1; Pp3c15_25560V3.1; Pp3c15_25560.
DR Gramene; Pp3c15_25560V3.2; Pp3c15_25560V3.2; Pp3c15_25560.
DR InParanoid; A0A2K1JEJ8; -.
DR OMA; KWPETFG; -.
DR OrthoDB; 3266779at2759; -.
DR Proteomes; UP000006727; Chromosome 15.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0003994; F:aconitate hydratase activity; IBA:GO_Central.
DR GO; GO:0030350; F:iron-responsive element binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006101; P:citrate metabolic process; IBA:GO_Central.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR CDD; cd01586; AcnA_IRP; 1.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01341; aconitase_1; 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000006727}.
FT DOMAIN 194..697
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 826..954
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
FT REGION 51..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 106..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1026 AA; 112233 MW; E4C1C91B96A403B2 CRC64;
MRLSRLPLPP RLPDRSFTMP LFMYVCRSLR QHGCSSKFPL SLGHRAASSA ASAGSIRSGV
PSFRSTPSQP RLSPDSAGSR LFRTIVSASY SADECRAAVA SVGRSSPRAL SPTMVPTTTT
VPGSRMASTQ ASENPFSSLI TDLPKPDGGS YGKFYSLVKL NDPRVDSLPY SIRYLLEAAI
RNCDNFQVTK EDVEKIVDWE KTAPKQVEIP FKPARVILQD FTGVPAVVDL AAMRDAITRL
GGDPDRINPL VPVDLVIDHS VQVDVARSAN ALQANMELEF SRNKERFGFL KWGATAFKNM
LVVPPGSGIV HQVNLEYLAR VVFNSNGILY PDTLVGTDSH TTMIDGLGVA GWGVGGIEAE
AAMLGQPMSM VLPGVVGFKL NGKLRTGVTA TDLVLTVTQI LRKFGVVGKF VEFYGKGMSE
LTLADRATIA NMSPEYGATM GFFPVDRVSL DYLKMTGRDE KKVEEIEAYL RANNLFIDHE
KPRKDNTYSA YLELDLDTVE PCISGPKRPH DRVAIKDMKQ DWQSCLDNKV GFKGFAIPKD
QQEKVAKFTY EGKPAELRHG SVVIAAITSC TNTSNPSVML GAGLVAKKAT ELGLEVKPWV
KTSLAPGSGV VTKYLEKSGL NKYLDKQGFS LVGYGCTTCI GNSGDVHEAV AEAIAANDMV
AAAVLSGNRN FEGRVHPLTR ANYLASPPLV VAYAFAGTVN IDFEKDPIGV GKDGKNVFLR
DIWPSNQEVA EVVATSVLPE MFQETYQTIT QGNTMWNGLD VPAGAQYAWD PNSTYVHEPP
FFKTMSKDPP GGMSVKDAFC LLNFGDSITT DHISPAGNIN KDSPAAKYLM DRGVERKDFN
SYGSRRGNDE IMVRGTFANI RIVNKFLKGE VGPKTVHVPT QEKMFIYDAA MKYKEEGHDT
IILAGAEYGS GSSRDWAAKG PYMQGVKAVI AKSFERIHRS NLVGMGLIPL CFKEGEDAET
LGLTGFERYT IEMPPLKDIK PGMDIRVKTD NNKEFMCVLR FDTQVELTYF SHGGILQYVL
RQLLNK
//
