ID A0A2K1JML1_PHYPA Unreviewed; 419 AA.
AC A0A2K1JML1;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Vacuolar cation/proton exchanger {ECO:0000256|RuleBase:RU365028};
GN ORFNames=PHYPA_017608 {ECO:0000313|EMBL:PNR42778.1};
OS Physcomitrium patens (Spreading-leaved earth moss) (Physcomitrella patens).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Bryophyta;
OC Bryophytina; Bryopsida; Funariidae; Funariales; Funariaceae; Physcomitrium.
OX NCBI_TaxID=3218 {ECO:0000313|EMBL:PNR42778.1};
RN [1] {ECO:0000313|EMBL:PNR42778.1, ECO:0000313|Proteomes:UP000006727}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Gransden 2004 {ECO:0000313|EnsemblPlants:Pp3c13_20070V3.1,
RC ECO:0000313|Proteomes:UP000006727};
RX PubMed=18079367; DOI=10.1126/science.1150646;
RA Rensing S.A., Lang D., Zimmer A.D., Terry A., Salamov A., Shapiro H.,
RA Nishiyama T., Perroud P.-F., Lindquist E.A., Kamisugi Y., Tanahashi T.,
RA Sakakibara K., Fujita T., Oishi K., Shin-I T., Kuroki Y., Toyoda A.,
RA Suzuki Y., Hashimoto S.-I., Yamaguchi K., Sugano S., Kohara Y.,
RA Fujiyama A., Anterola A., Aoki S., Ashton N., Barbazuk W.B., Barker E.,
RA Bennetzen J.L., Blankenship R., Cho S.H., Dutcher S.K., Estelle M.,
RA Fawcett J.A., Gundlach H., Hanada K., Heyl A., Hicks K.A., Hughes J.,
RA Lohr M., Mayer K., Melkozernov A., Murata T., Nelson D.R., Pils B.,
RA Prigge M., Reiss B., Renner T., Rombauts S., Rushton P.J., Sanderfoot A.,
RA Schween G., Shiu S.-H., Stueber K., Theodoulou F.L., Tu H., Van de Peer Y.,
RA Verrier P.J., Waters E., Wood A., Yang L., Cove D., Cuming A.C., Hasebe M.,
RA Lucas S., Mishler B.D., Reski R., Grigoriev I.V., Quatrano R.S.,
RA Boore J.L.;
RT "The Physcomitrella genome reveals evolutionary insights into the conquest
RT of land by plants.";
RL Science 319:64-69(2008).
RN [2] {ECO:0000313|EMBL:PNR42778.1, ECO:0000313|Proteomes:UP000006727}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Gransden 2004 {ECO:0000313|EnsemblPlants:Pp3c13_20070V3.1,
RC ECO:0000313|Proteomes:UP000006727};
RX PubMed=29237241; DOI=10.1111/tpj.13801;
RA Lang D., Ullrich K.K., Murat F., Fuchs J., Jenkins J., Haas F.B.,
RA Piednoel M., Gundlach H., Van Bel M., Meyberg R., Vives C., Morata J.,
RA Symeonidi A., Hiss M., Muchero W., Kamisugi Y., Saleh O., Blanc G.,
RA Decker E.L., van Gessel N., Grimwood J., Hayes R.D., Graham S.W.,
RA Gunter L.E., McDaniel S.F., Hoernstein S.N.W., Larsson A., Li F.W.,
RA Perroud P.F., Phillips J., Ranjan P., Rokshar D.S., Rothfels C.J.,
RA Schneider L., Shu S., Stevenson D.W., Thummler F., Tillich M.,
RA Villarreal Aguilar J.C., Widiez T., Wong G.K., Wymore A., Zhang Y.,
RA Zimmer A.D., Quatrano R.S., Mayer K.F.X., Goodstein D., Casacuberta J.M.,
RA Vandepoele K., Reski R., Cuming A.C., Tuskan G.A., Maumus F., Salse J.,
RA Schmutz J., Rensing S.A.;
RT "The Physcomitrella patens chromosome-scale assembly reveals moss genome
RT structure and evolution.";
RL Plant J. 93:515-533(2018).
RN [3] {ECO:0000313|EnsemblPlants:Pp3c13_20070V3.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (DEC-2020) to UniProtKB.
CC -!- FUNCTION: Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+)
CC and other metal ions into vacuoles using the proton gradient formed by
CC H(+)-ATPase and H(+)-pyrophosphatase. {ECO:0000256|RuleBase:RU365028}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Vacuole membrane
CC {ECO:0000256|RuleBase:RU365028}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. Cation/proton exchanger (CAX) subfamily.
CC {ECO:0000256|ARBA:ARBA00008248}.
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DR EMBL; ABEU02000013; PNR42778.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K1JML1; -.
DR STRING; 3218.A0A2K1JML1; -.
DR PaxDb; 3218-PP1S223_68V6-1; -.
DR EnsemblPlants; Pp3c13_20070V3.1; Pp3c13_20070V3.1; Pp3c13_20070.
DR EnsemblPlants; Pp3c13_20070V3.2; Pp3c13_20070V3.2; Pp3c13_20070.
DR Gramene; Pp3c13_20070V3.1; Pp3c13_20070V3.1; Pp3c13_20070.
DR Gramene; Pp3c13_20070V3.2; Pp3c13_20070V3.2; Pp3c13_20070.
DR InParanoid; A0A2K1JML1; -.
DR OrthoDB; 276371at2759; -.
DR Proteomes; UP000006727; Chromosome 13.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006874; P:intracellular calcium ion homeostasis; IBA:GO_Central.
DR Gene3D; 1.20.1420.30; NCX, central ion-binding region; 1.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR NCBIfam; TIGR00378; cax; 1.
DR PANTHER; PTHR31503; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR PANTHER; PTHR31503:SF22; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
PE 3: Inferred from homology;
KW Antiport {ECO:0000256|RuleBase:RU365028};
KW Calcium {ECO:0000256|RuleBase:RU365028};
KW Calcium transport {ECO:0000256|RuleBase:RU365028};
KW Ion transport {ECO:0000256|RuleBase:RU365028};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU365028};
KW Reference proteome {ECO:0000313|Proteomes:UP000006727};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU365028};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU365028}; Transport {ECO:0000256|RuleBase:RU365028};
KW Vacuole {ECO:0000256|RuleBase:RU365028}.
FT TRANSMEM 51..73
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 79..98
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 110..131
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 143..164
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 184..202
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 214..232
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 263..282
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 294..312
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 324..352
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 364..382
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT DOMAIN 79..234
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
FT DOMAIN 263..401
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
SQ SEQUENCE 419 AA; 45018 MW; 582FEB0626F6713C CRC64;
MGNSAAGRSK TSEMKNGDCS NAKSTAYVSL AARKLSFLGA KRRFVGSAKN VLFGTVLNVL
IVAVPLAILS NYLRIGNSWV FGLSLIGIIP LAERLGFVTE QLAYFTGPTV GGLLNATFGN
ATEMIICVFA LQNNMVRVVQ LSLLGSILSN MLLVLGCSFL FGGFATSKKE QTFDKSAAHL
NSSLLLMAVM CVLFPAALHA TGTEVHLGQS ELTLSRITSC VMLVAYAGYI WFQIRNPLSL
LEEDVEAGED GESEEFEVDA WSAVFWLAFL TVFISILSKY IVDAIEGASV DWHIPVAFIS
VIILPVVGNA AEHTSAIIFA MKDNLALSLA VAIGSSTQIS MLVIPVCVIL GWPMGVTLDL
NFELFETAVL FISVLVVTFM CLEGSANYFK GVMLVLCYFI VGASFFVHVD PPTQLKPSA
//
