ID A0A2K1Q311_9GAMM Unreviewed; 439 AA.
AC A0A2K1Q311;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Isocitrate lyase {ECO:0000256|ARBA:ARBA00017446};
DE EC=4.1.3.1 {ECO:0000256|ARBA:ARBA00012909};
DE AltName: Full=Isocitrase {ECO:0000256|ARBA:ARBA00031022};
DE AltName: Full=Isocitratase {ECO:0000256|ARBA:ARBA00031921};
GN ORFNames=Lysil_1065 {ECO:0000313|EMBL:PNS09436.1};
OS Lysobacter silvestris.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Lysobacter.
OX NCBI_TaxID=1645665 {ECO:0000313|EMBL:PNS09436.1, ECO:0000313|Proteomes:UP000236220};
RN [1] {ECO:0000313|EMBL:PNS09436.1, ECO:0000313|Proteomes:UP000236220}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AM20-91 {ECO:0000313|EMBL:PNS09436.1,
RC ECO:0000313|Proteomes:UP000236220};
RA Zhang D.-C., Albuquerque L., Franca L., Froufe H.J.C., Barroso C., Egas C.,
RA Da Costa M., Margesin R.;
RT "Lysobacter sylvestris genome.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-threo-isocitrate = glyoxylate + succinate;
CC Xref=Rhea:RHEA:13245, ChEBI:CHEBI:15562, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:36655; EC=4.1.3.1;
CC Evidence={ECO:0000256|ARBA:ARBA00023531};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR001362-3};
CC Note=Can also use Mn(2+) ion. {ECO:0000256|PIRSR:PIRSR001362-3};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PNS09436.1}.
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DR EMBL; NPZB01000001; PNS09436.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K1Q311; -.
DR OrthoDB; 8629576at2; -.
DR Proteomes; UP000236220; Unassembled WGS sequence.
DR GO; GO:0004451; F:isocitrate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR CDD; cd00377; ICL_PEPM; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR InterPro; IPR039556; ICL/PEPM.
DR InterPro; IPR006254; Isocitrate_lyase.
DR InterPro; IPR018523; Isocitrate_lyase_ph_CS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01346; isocit_lyase; 2.
DR PANTHER; PTHR21631:SF3; BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN; 1.
DR PANTHER; PTHR21631; ISOCITRATE LYASE/MALATE SYNTHASE; 1.
DR Pfam; PF00463; ICL; 2.
DR PIRSF; PIRSF001362; Isocit_lyase; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00161; ISOCITRATE_LYASE; 1.
PE 4: Predicted;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:PNS09436.1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR001362-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001362-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000236220}.
FT ACT_SITE 193
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-1"
FT BINDING 94..96
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 155
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-3"
FT BINDING 194..195
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 230
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 315..319
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 349
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
SQ SEQUENCE 439 AA; 47851 MW; 3CFB1DB8B5B2E1FA CRC64;
MSTTSLSSAL PSAEQLRLEW SNNPRWAGIR RDYSAEDVVR LRGTVHVEHS LARLGAGKLW
TSLQREDFVN ALGALTGNQA MQQVKAGLKA IYLSGWQVAA DANLAGEMYP DQSLYPANSV
PQVVKRINNT LLRADQLNHA EGDDSIDYLQ PIVADAEAGF GGVLNAFELM KSMIEAGAAG
VHFEDQLASV KKCGHMGGKV LVPTREAIEK LTAARLAADV MGVPTLIVAR TDAEAADLLT
SDVDANDLPF CTGERTVEGF YKTHKGLDQA ISRGLAYAPY ADLVWCETGK PDLEFARKFA
EAIHAKYPGK LLAYNCSPSF NWKKNLDDAT IARFQHEIAS YGYKFQFITL AGFHALNYGM
FDLAHGYARR QMSAFVELQE REFAAADRGF TAVKHQREVG TGYFDAVTQT IQGGKSSTVA
LKGSTEEEQF QPQAVAVAA
//
