ID A0A2K1WXR3_POPTR Unreviewed; 703 AA.
AC A0A2K1WXR3;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 2.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=tRNA(Ile)-lysidine synthetase {ECO:0000256|ARBA:ARBA00013267};
DE EC=6.3.4.19 {ECO:0000256|ARBA:ARBA00013267};
GN ORFNames=POPTR_018G081500 {ECO:0000313|EMBL:PNS93311.2};
OS Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp.
OS trichocarpa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus.
OX NCBI_TaxID=3694 {ECO:0000313|EMBL:PNS93311.2, ECO:0000313|Proteomes:UP000006729};
RN [1] {ECO:0000313|EMBL:PNS93311.2, ECO:0000313|Proteomes:UP000006729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nisqually {ECO:0000313|Proteomes:UP000006729};
RX PubMed=16973872; DOI=10.1126/science.1128691;
RA Tuskan G.A., Difazio S., Jansson S., Bohlmann J., Grigoriev I.,
RA Hellsten U., Putnam N., Ralph S., Rombauts S., Salamov A., Schein J.,
RA Sterck L., Aerts A., Bhalerao R.R., Bhalerao R.P., Blaudez D., Boerjan W.,
RA Brun A., Brunner A., Busov V., Campbell M., Carlson J., Chalot M.,
RA Chapman J., Chen G.L., Cooper D., Coutinho P.M., Couturier J., Covert S.,
RA Cronk Q., Cunningham R., Davis J., Degroeve S., Dejardin A.,
RA Depamphilis C., Detter J., Dirks B., Dubchak I., Duplessis S., Ehlting J.,
RA Ellis B., Gendler K., Goodstein D., Gribskov M., Grimwood J., Groover A.,
RA Gunter L., Hamberger B., Heinze B., Helariutta Y., Henrissat B.,
RA Holligan D., Holt R., Huang W., Islam-Faridi N., Jones S.,
RA Jones-Rhoades M., Jorgensen R., Joshi C., Kangasjarvi J., Karlsson J.,
RA Kelleher C., Kirkpatrick R., Kirst M., Kohler A., Kalluri U., Larimer F.,
RA Leebens-Mack J., Leple J.C., Locascio P., Lou Y., Lucas S., Martin F.,
RA Montanini B., Napoli C., Nelson D.R., Nelson C., Nieminen K., Nilsson O.,
RA Pereda V., Peter G., Philippe R., Pilate G., Poliakov A., Razumovskaya J.,
RA Richardson P., Rinaldi C., Ritland K., Rouze P., Ryaboy D., Schmutz J.,
RA Schrader J., Segerman B., Shin H., Siddiqui A., Sterky F., Terry A.,
RA Tsai C.J., Uberbacher E., Unneberg P., Vahala J., Wall K., Wessler S.,
RA Yang G., Yin T., Douglas C., Marra M., Sandberg G., Van de Peer Y.,
RA Rokhsar D.;
RT "The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).";
RL Science 313:1596-1604(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP +
CC diphosphate + H(+) + lysidine(34) in tRNA(Ile2);
CC Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665,
CC ChEBI:CHEBI:456215; EC=6.3.4.19;
CC Evidence={ECO:0000256|ARBA:ARBA00000047};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; CM009307; PNS93311.2; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K1WXR3; -.
DR STRING; 3694.A0A2K1WXR3; -.
DR EnsemblPlants; Potri.018G081500.2.v4.1; Potri.018G081500.2.v4.1; Potri.018G081500.v4.1.
DR Gramene; Potri.018G081500.2.v4.1; Potri.018G081500.2.v4.1; Potri.018G081500.v4.1.
DR InParanoid; A0A2K1WXR3; -.
DR OrthoDB; 460053at2759; -.
DR Proteomes; UP000006729; Chromosome 18.
DR ExpressionAtlas; A0A2K1WXR3; baseline.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:InterPro.
DR GO; GO:0009658; P:chloroplast organization; IEA:EnsemblPlants.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IEA:EnsemblPlants.
DR GO; GO:0010098; P:suspensor development; IEA:EnsemblPlants.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd01992; PP-ATPase; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_01161; tRNA_Ile_lys_synt; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR011063; TilS/TtcA_N.
DR InterPro; IPR012094; tRNA_Ile_lys_synt.
DR InterPro; IPR012795; tRNA_Ile_lys_synt_N.
DR NCBIfam; TIGR02432; lysidine_TilS_N; 1.
DR PANTHER; PTHR43033; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR PANTHER; PTHR43033:SF1; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR Pfam; PF01171; ATP_bind_3; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Reference proteome {ECO:0000313|Proteomes:UP000006729}.
FT DOMAIN 84..290
FT /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01171"
SQ SEQUENCE 703 AA; 78668 MW; 60D9841AEEC8464A CRC64;
MARGLILTTQ ARATTTSFTT RLSVSKPKCK NPLPYRLHLP SRVSSTRFFC CKCSVSQDPI
VITEYKQSFS QRMAMAGLKP HHRIAIGVSG GPDSMALCFL TAGWKTDGAN AVGKSDDGFI
NGILGVIVDH GLREESNEEA HIVSSRVTEM GIRCEIAKCS WLDGKPKQGH LLEEAREKRY
EVFQNVCTKH QIEVLLIAHH ADDQAELFIL RLSRNSGVLG LAGMAFTSQM FSKSTHLYRE
GSKNKGILIV RPLLHFSKEI LYKICQESGQ DWVEDPTNQN TVYARNRIRM SLGNLSSYTF
QSELQGVISA CRRTRAYVDQ ICNNLINQAV TIIDQHGYAI IDLEILNPSK VTDICLSKFV
ALILQYVSQR NRPIRGSTSK LLLHYIRTVP CKTSFTAAGC YLCPAPRSRG TKILVCCSVD
CPLNSKMELI YPFLNGEQKH YFRNELEQII ADGKSYSNHF VPDASDVHFL DASESVISEA
KTLNIISEST YRDILLLKRE EIKHFKHKVE DKVDYKSKNK VESIIASPSE LLQPGKACYF
MNRFWITWKL SNHVSVGEGT ENCVADLGGE SQECHSCSCR IGHDKVAEVR RMSESDWLYL
AKLSKCPSLD NLQQQKVLSS STMEQISEKR SLHLENLELS AQKALEVLKS IPVAARRSLP
VLVNHQGLLL SIPSIGFKHC PCLMVSCEFK PIVPLGGGHS SFM
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