ID A0A2K2B0D1_POPTR Unreviewed; 1219 AA.
AC A0A2K2B0D1;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 2.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Telomerase reverse transcriptase {ECO:0000256|ARBA:ARBA00016182, ECO:0000256|RuleBase:RU365061};
DE EC=2.7.7.49 {ECO:0000256|ARBA:ARBA00012493, ECO:0000256|RuleBase:RU365061};
DE AltName: Full=Telomerase catalytic subunit {ECO:0000256|RuleBase:RU365061};
GN ORFNames=POPTR_003G022600 {ECO:0000313|EMBL:PNT43237.2};
OS Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp.
OS trichocarpa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus.
OX NCBI_TaxID=3694 {ECO:0000313|EMBL:PNT43237.2, ECO:0000313|Proteomes:UP000006729};
RN [1] {ECO:0000313|EMBL:PNT43237.2, ECO:0000313|Proteomes:UP000006729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nisqually {ECO:0000313|Proteomes:UP000006729};
RX PubMed=16973872; DOI=10.1126/science.1128691;
RA Tuskan G.A., Difazio S., Jansson S., Bohlmann J., Grigoriev I.,
RA Hellsten U., Putnam N., Ralph S., Rombauts S., Salamov A., Schein J.,
RA Sterck L., Aerts A., Bhalerao R.R., Bhalerao R.P., Blaudez D., Boerjan W.,
RA Brun A., Brunner A., Busov V., Campbell M., Carlson J., Chalot M.,
RA Chapman J., Chen G.L., Cooper D., Coutinho P.M., Couturier J., Covert S.,
RA Cronk Q., Cunningham R., Davis J., Degroeve S., Dejardin A.,
RA Depamphilis C., Detter J., Dirks B., Dubchak I., Duplessis S., Ehlting J.,
RA Ellis B., Gendler K., Goodstein D., Gribskov M., Grimwood J., Groover A.,
RA Gunter L., Hamberger B., Heinze B., Helariutta Y., Henrissat B.,
RA Holligan D., Holt R., Huang W., Islam-Faridi N., Jones S.,
RA Jones-Rhoades M., Jorgensen R., Joshi C., Kangasjarvi J., Karlsson J.,
RA Kelleher C., Kirkpatrick R., Kirst M., Kohler A., Kalluri U., Larimer F.,
RA Leebens-Mack J., Leple J.C., Locascio P., Lou Y., Lucas S., Martin F.,
RA Montanini B., Napoli C., Nelson D.R., Nelson C., Nieminen K., Nilsson O.,
RA Pereda V., Peter G., Philippe R., Pilate G., Poliakov A., Razumovskaya J.,
RA Richardson P., Rinaldi C., Ritland K., Rouze P., Ryaboy D., Schmutz J.,
RA Schrader J., Segerman B., Shin H., Siddiqui A., Sterky F., Terry A.,
RA Tsai C.J., Uberbacher E., Unneberg P., Vahala J., Wall K., Wessler S.,
RA Yang G., Yin T., Douglas C., Marra M., Sandberg G., Van de Peer Y.,
RA Rokhsar D.;
RT "The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).";
RL Science 313:1596-1604(2006).
CC -!- FUNCTION: Telomerase is a ribonucleoprotein enzyme essential for the
CC replication of chromosome termini in most eukaryotes. It elongates
CC telomeres. It is a reverse transcriptase that adds simple sequence
CC repeats to chromosome ends by copying a template sequence within the
CC RNA component of the enzyme. {ECO:0000256|RuleBase:RU365061}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.49;
CC Evidence={ECO:0000256|ARBA:ARBA00024557,
CC ECO:0000256|RuleBase:RU365061};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365061}.
CC Chromosome, telomere {ECO:0000256|RuleBase:RU365061}.
CC -!- SIMILARITY: Belongs to the reverse transcriptase family. Telomerase
CC subfamily. {ECO:0000256|ARBA:ARBA00008001,
CC ECO:0000256|RuleBase:RU365061}.
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DR EMBL; CM009292; PNT43237.2; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K2B0D1; -.
DR Proteomes; UP000006729; Chromosome 3.
DR ExpressionAtlas; A0A2K2B0D1; baseline and differential.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003721; F:telomerase RNA reverse transcriptase activity; IEA:InterPro.
DR CDD; cd01648; TERT; 1.
DR Gene3D; 1.10.132.70; -; 1.
DR Gene3D; 1.10.357.90; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR000477; RT_dom.
DR InterPro; IPR021891; Telomerase_RBD.
DR InterPro; IPR003545; Telomerase_RT.
DR InterPro; IPR049139; TERT_C.
DR PANTHER; PTHR12066; TELOMERASE REVERSE TRANSCRIPTASE; 1.
DR PANTHER; PTHR12066:SF0; TELOMERASE REVERSE TRANSCRIPTASE; 1.
DR Pfam; PF00078; RVT_1; 1.
DR Pfam; PF12009; Telomerase_RBD; 1.
DR Pfam; PF21399; TERT_C; 1.
DR PRINTS; PR01365; TELOMERASERT.
DR SMART; SM00975; Telomerase_RBD; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 3: Inferred from homology;
KW Chromosome {ECO:0000256|ARBA:ARBA00022454, ECO:0000256|RuleBase:RU365061};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU365061};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU365061};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU365061};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU365061};
KW Reference proteome {ECO:0000313|Proteomes:UP000006729};
KW RNA-directed DNA polymerase {ECO:0000256|RuleBase:RU365061};
KW Telomere {ECO:0000256|ARBA:ARBA00022895, ECO:0000256|RuleBase:RU365061};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365061}.
FT DOMAIN 651..1025
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000259|PROSITE:PS50878"
SQ SEQUENCE 1219 AA; 140798 MW; E43E75170EDF08EE CRC64;
MSKKKTKGRV PEVLWRVFRY RARTLSNTVT SLIANPPSSF VLFKADDPAN YRKLLKDCYI
VLSDNAPPVA HFNLENRWPQ PLIVSRIIEF IITEQPLSNN VLCSGYDKCL RSSPIAEVLT
SSAWTLLLER VGDEFMNYLL KYSSMFLPLP RQQHQQVAGP PITDFVFQSS RRKGHSPNLV
GLKRKRDWDD KAADAASLML KRHQLTADAS LSYDRTTYPV IDFDQDKGLS EKTGVAAVNR
EGHSNEKLPG LYQCTLRSRK PFGWQRRRCK KQRSLDAKET DGKTCYIMNE GSSPERLPLE
FRNSSSPGKM LPQCCCHLVL QAPNMKGIQI DRRPIFYNSK HPSSMLPKNH VLMCLKPNLI
GSKSLIQSIF GLSDENVSAP SMPLSYSKDF CLLGSTCLYH SLVKLVKVLV RQTQCCKYAR
LIHRHCFVSS LNQNAIENSN SVFEGCNLER EFSGKSHAVV NERRDKTLET TDHQIVTLKS
YCSKSQVVSF IWAVCRNVVP PELLGNHVNR RILRRNLAKF IWLRKFEKFS LKQCMHKLKT
SGFPFLSDKH CSCFLDTHML NNMPGHTAGL HKDLYKWNGA ANDLKHLLVQ KWIYWFFSSL
VVPLVQANFY VTESEHGKHE IYYYRKSIWE KLRNRAISCL KDQNYQCLDE ASAESITGCR
LFGFSKLRLL PKKNGIRMIA NLKASSKMPK KSTSELQSFR MQRKKQFITK VVKCEHFKSV
NCVLRETHAV LKGIQLKEPD KLGSSVFDYN DIYRKLCQFK ICLNKRSNIM PDVFIVVSDV
SKAFDSIDQD KLLCVMEDVI YEDQYLLKRF CQVVSTKKSL WAHERLMSRD QNITTGLTRF
SSSFRFGSLH TVLVNQGRSR YLKREQLLSN LTEHVKRNVL QFDDKFYLQR KGIPQGSILS
SLLCSLYYGH LERNVIFPFL EDLSRRHCYQ DASAIGSSSR DKVISSPHYM LLRFIDDFLF
ISTSKKQAAG LFSQLRSGFR DYNCFMNEEK FCLNLDTEQV SGLQSNKLYV GGDGISFLRW
SGLLLNSCTL EVQADYTRYL NNHLNSTLTV CWQGKPCQHL ERKLWNFMRP KCHPIFFDSN
INSAPVVRLN IYQAFLLCAM KFHCYVSSMS FHCHLSATFY ANMIERSLRY MYVLIKRRMR
SMHLGSCFHP ILQLEVGEVE WLGLNAYIQV LKRKQSRYKE LLSRLSLKLS KHRIGGSTSS
HLKYAVDSSH SSLLWKIKY
//
