ID A0A2K2FLG5_9FIRM Unreviewed; 734 AA.
AC A0A2K2FLG5;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=CRISPR-associated helicase/endonuclease Cas3 {ECO:0008006|Google:ProtNLM};
GN ORFNames=CDQ84_02275 {ECO:0000313|EMBL:PNU01232.1};
OS Pseudoclostridium thermosuccinogenes.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Pseudoclostridium.
OX NCBI_TaxID=84032 {ECO:0000313|EMBL:PNU01232.1, ECO:0000313|Proteomes:UP000236151};
RN [1] {ECO:0000313|EMBL:PNU01232.1, ECO:0000313|Proteomes:UP000236151}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5806 {ECO:0000313|EMBL:PNU01232.1,
RC ECO:0000313|Proteomes:UP000236151};
RA Koendjbiharie J.G., van Kranenburg R.;
RT "Investigating the central metabolism of Clostridium thermosuccinogenes.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PNU01232.1}.
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DR EMBL; NIOJ01000003; PNU01232.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K2FLG5; -.
DR KEGG; cthd:CDO33_11195; -.
DR OrthoDB; 9810236at2; -.
DR Proteomes; UP000236151; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR CDD; cd17930; DEXHc_cas3; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR006674; HD_domain.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF01966; HD; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000236151}.
FT DOMAIN 11..178
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 234..420
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 734 AA; 83868 MW; 4D9183D3E39A3619 CRC64;
MYYAHTKVGL PEDEWQKLSD HLTGTSELAG KKASKFNGEE YGKVAGLLHD AGKYSIEFQK
RLRGSKVPVD HSTAGAKEAF EKYGLPGLIL AYVVAGHHTG MPDWIDPGSS SSLSERIENA
DLPDYKEFHK EISLTDKLPP LPIKIHGRDR EEIRKSTGFS LSFFTRMLYS CLVDADYRDT
ERFIDPQKSK NRDLKINLSQ LLQNLDTYME AKCSKSSEGY INRKRNEIRI ACIEKSSLPK
GMFSLKVPTG GGKTLSSLEF AIRHAICHNM DRVIYAIPYT SIIEQNAQVF REALGEEFVL
EHHSNFLFDR DEDDLNYEDN PLRLAAEDWN IPVVVTTNVQ FFESLFSNRS SRCRKLHNLC
NSVIVLDEAQ MIPIEFLKPC LAAICELIRN YGCSVVLCTA TQPDINDFLP VDMKPREIIH
NADELFRSLK RVKTSYVGRM TDEELSTKLK DCRQVLCIVN TKSRASQIYD LINDGEGIYH
LSTFMCPIHR NKKLNEIKEC LKASKQNSNL PCRVVSTQLV EAGVDLDFPT VYREIAGIDS
INQAAGRCNR EGRLEWGRVF VFEPEKLLAS DSFLARAASI GRSVLRKYDD PMLLEAIKEY
FRCLYHQDEN LLDKHRIIEA LSECGMSLQF PFATIARLFR LISNNQVSLI IPYDERCRKM
LSDAQWLDKP EKLARKLQPY VVPLEVYQFK DLEKNNLIET VAGVFYVLSD MKFYDDNKGL
ILPKGTVEQD ILIY
//
