ID A0A2K3DQP7_CHLRE Unreviewed; 934 AA.
AC A0A2K3DQP7;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=CHLRE_06g296150v5 {ECO:0000313|EMBL:PNW82827.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055 {ECO:0000313|EMBL:PNW82827.1, ECO:0000313|Proteomes:UP000006906};
RN [1] {ECO:0000313|EMBL:PNW82827.1, ECO:0000313|Proteomes:UP000006906}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503 {ECO:0000313|Proteomes:UP000006906};
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
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DR EMBL; CM008967; PNW82827.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K3DQP7; -.
DR STRING; 3055.A0A2K3DQP7; -.
DR PaxDb; 3055-EDP05204; -.
DR EnsemblPlants; PNW82827; PNW82827; CHLRE_06g296150v5.
DR Gramene; PNW82827; PNW82827; CHLRE_06g296150v5.
DR InParanoid; A0A2K3DQP7; -.
DR OrthoDB; 317209at2759; -.
DR Proteomes; UP000006906; Chromosome 6.
DR ExpressionAtlas; A0A2K3DQP7; baseline.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR GO; GO:0043139; F:5'-3' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR47642; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR47642:SF5; ATP-DEPENDENT DNA HELICASE; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000006906};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 162..184
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 502..535
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
FT REGION 71..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 721..748
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 934 AA; 100224 MW; 8478DAE84CA623A4 CRC64;
MLNKSSAALG CDRKGRLAGR CFVPKLQPAR LGLAPLPLLG VTSSPDTTAR LLRPALLSRV
PRSLPEVVTA AKARAARAKR GSSSSSKTAD GRHGPRRGSG SSTLKLPPSF LPSPEQARAI
ATVLSGRNLF LTGCAGTGKS ATLLALRAHL AGRYGGEYDR RVAVVAMTGL AATIVGGITI
HSLLKLRRVD SFRDLERMVN DPTVLSQLLE LRTLILDEAG MMSAELMQAL DCYLTCARKK
AAEEEASRNK LKGEAREAFL ARFDQPFGGL QIILSGDFFQ LAPIENKDPV GHTHATYRPP
TPSDQPFRNR GFMFQAPVFQ YGGFTLVELT KVYRQDEVAF VDLLNAVRCG QEAACAEAKR
QLLAQCAAPL DESDGIKPTL LYSKNADVGQ KNEEELQQLP GKPVVLDALD AVRVTHDPPL
SVYQPLRQEG LVGDGAWEGL AAALEAAESD HLVTLAAHLR SNAAALRRAK ELRAAWQRHA
RDHLRTVANG GGGLFRECQA ADKVQLKVGA QVMLVWNVDT EQGLVNGSRG VVERFEVAGE
GDISRAVARS GGADQVDCGP ELREWFQLNG QVPVVRLTNG SEVSVLPVAF TSNVPGYGAC
VRVQVPLKLA WAITVHKSQG MTLDKAVVNL RDFFGHGMLY TALSRSRGVK GLQITGDSSR
RMDARVMRWW EAHRAGRLYV NPDARPDSQG PWCWRDPLQP CSKPQNAAAR LWWELGPERY
HEATRQERER QEAEVRAAEA AVAAEAAAAV AEAAKAAEAA KAAEVAAAEA GAEPESEVVV
EVQELLPHEQ AAATTLEVFT DAAPAEVAAE LPPAVVAAGS LLGARVASGM EPVATAADVV
PPSTVAAATA VAPPDAAVAA AAPPAVVGSP WRQRLRRQRQ LLGGVESELQ LLVTRRGAVR
KDELAGPLRR LAELRAEMLA LEEEEEEEEE EEAA
//
