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Database: UniProt
Entry: A0A2K3E5T4_CHLRE
LinkDB: A0A2K3E5T4_CHLRE
Original site: A0A2K3E5T4_CHLRE 
ID   A0A2K3E5T4_CHLRE        Unreviewed;      1089 AA.
AC   A0A2K3E5T4;
DT   28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT   28-MAR-2018, sequence version 1.
DT   05-JUN-2019, entry version 10.
DE   RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE            EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN   ORFNames=CHLRE_01g015250v5 {ECO:0000313|EMBL:PNW88124.1};
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae;
OC   Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055 {ECO:0000313|EMBL:PNW88124.1, ECO:0000313|Proteomes:UP000006906};
RN   [1] {ECO:0000313|EMBL:PNW88124.1, ECO:0000313|Proteomes:UP000006906}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503 {ECO:0000313|Proteomes:UP000006906};
RX   PubMed=17932292; DOI=10.1126/science.1143609;
RA   Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA   Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K.,
RA   Marechal-Drouard L., Marshall W.F., Qu L.H., Nelson D.R.,
RA   Sanderfoot A.A., Spalding M.H., Kapitonov V.V., Ren Q., Ferris P.,
RA   Lindquist E., Shapiro H., Lucas S.M., Grimwood J., Schmutz J.,
RA   Cardol P., Cerutti H., Chanfreau G., Chen C.L., Cognat V., Croft M.T.,
RA   Dent R., Dutcher S., Fernandez E., Fukuzawa H., Gonzalez-Ballester D.,
RA   Gonzalez-Halphen D., Hallmann A., Hanikenne M., Hippler M., Inwood W.,
RA   Jabbari K., Kalanon M., Kuras R., Lefebvre P.A., Lemaire S.D.,
RA   Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L., Mittag M.,
RA   Mittelmeier T., Moroney J.V., Moseley J., Napoli C., Nedelcu A.M.,
RA   Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G., Purton S.,
RA   Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L., Schroda M.,
RA   Stern D., Umen J., Willows R., Wilson N., Zimmer S.L., Allmer J.,
RA   Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA   Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA   Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA   Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P.,
RA   Jorgensen R., Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T.,
RA   Brokstein P., Dubchak I., Goodstein D., Hornick L., Huang Y.W.,
RA   Jhaveri J., Luo Y., Martinez D., Ngau W.C., Otillar B., Poliakov A.,
RA   Porter A., Szajkowski L., Werner G., Zhou K., Grigoriev I.V.,
RA   Rokhsar D.S., Grossman A.R.;
RT   "The Chlamydomonas genome reveals the evolution of key animal and
RT   plant functions.";
RL   Science 318:245-250(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-
CC         COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7;
CC         Evidence={ECO:0000256|RuleBase:RU000442};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|RuleBase:RU000442};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU000442}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC       {ECO:0000256|RuleBase:RU000442}.
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DR   EMBL; CM008962; PNW88124.1; -; Genomic_DNA.
DR   EnsemblPlants; PNW88124; PNW88124; CHLRE_01g015250v5.
DR   Gramene; PNW88124; PNW88124; CHLRE_01g015250v5.
DR   OMA; CLVNYTE; -.
DR   Proteomes; UP000006906; Chromosome 1.
DR   ExpressionAtlas; A0A2K3E5T4; differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR042087; DNA_pol_B_C.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR025687; Znf-C4pol.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   Pfam; PF14260; zf-C4pol; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   4Fe-4S {ECO:0000256|RuleBase:RU000442};
KW   Complete proteome {ECO:0000313|Proteomes:UP000006906};
KW   DNA replication {ECO:0000256|RuleBase:RU000442};
KW   DNA-binding {ECO:0000256|RuleBase:RU000442};
KW   DNA-directed DNA polymerase {ECO:0000256|RuleBase:RU000442};
KW   Iron {ECO:0000256|RuleBase:RU000442};
KW   Iron-sulfur {ECO:0000256|RuleBase:RU000442};
KW   Metal-binding {ECO:0000256|RuleBase:RU000442};
KW   Nucleotidyltransferase {ECO:0000256|RuleBase:RU000442};
KW   Nucleus {ECO:0000256|RuleBase:RU000442};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006906};
KW   Transferase {ECO:0000256|RuleBase:RU000442};
KW   Zinc {ECO:0000256|RuleBase:RU000442};
KW   Zinc-finger {ECO:0000256|RuleBase:RU000442}.
FT   DOMAIN      113    458       DNA_pol_B_exo1. {ECO:0000259|Pfam:
FT                                PF03104}.
FT   DOMAIN      522    956       DNA_pol_B. {ECO:0000259|Pfam:PF00136}.
FT   DOMAIN      995   1067       zf-C4pol. {ECO:0000259|Pfam:PF14260}.
FT   REGION        1     50       Disordered. {ECO:0000256|MobiDB-lite:
FT                                A0A2K3E5T4}.
FT   COMPBIAS     31     45       Acidic. {ECO:0000256|MobiDB-lite:
FT                                A0A2K3E5T4}.
SQ   SEQUENCE   1089 AA;  120551 MW;  A9669C92640B9C8A CRC64;
     MADSQAKRYA GGGHYEGGPP AKRQATGPDP DDMDFIEDDP SGPPEPLEDV ELGEAGRNWV
     RPPVADFDPA STAIVFQQLE VDYYLAEPRA GVDTVPVEVK QVPVLRLFGV NAAGNSVCAL
     VHGFEPYFLI ERRPHWGADH IQALGLALNQ ELLGKAKGVG GPAVTRIEPA ERRSIWNFQP
     PGTSTAFLKI VVSVPNLVAP ARGLLEGGMS LSAVNDFVKC TTYESNVLFT LRFMVDCKVV
     GGNWVELPAG AYQLETARPM THCQIEAHIR YDKLISHAPD GEYSSVAPLR IMSIDIECCG
     RKGCFPEAKI DPVIQIASMV SVSGKMDGPV VKNIMTLGSC ASIVGAEVMS FNDEAEMLLR
     WRDLLVESDP DIIIGYNILN FDLPYLYERA DALKIGKKFQ VWGRVRGSHT RMRDGQFSSK
     AYGTHEYKDI TIEGRVQFDL LTAIQRDHKL SSYSLNSVSA HFLGEQKEDV HHSAIADLQA
     GNEETRRRLA VYCLKDAYLP QRLLDKLMYT YNYIEMARVT GVPLSYLLTR GQSIKVFSQL
     LRKAAAKGFV IPNIKVQGPV AGADGVGYEG ATVLDPKIGF YDRPVATLDF ASLYPSIMMA
     HNLCYTTLLT KDQLNIVPPE DREQSPSGDW FVRNSRQRGL LPEILEELLA ARKRAKNDLK
     AEKDPFKRAV LDGRQLALKV SANSVYGFTG ATVGKMPCLA ISASTTSYGR EMIMTTRQLV
     QEHYTRANGY ETDCEVIYGD TDSVMVNFKV ADVARAMELG REAAAVVSKA FPPPVKLEFE
     KVYYPYLLMN KKRYAGLLWT RPETHDKMDS KGIETVRRDN CLLVRNVVTT CLERILIGKD
     VQGAVGYVKG VIADLLMNKI DLSLLVISKG LTQDADEYEG KIAHVELAKK MRKRDPATAP
     AVGDRVPYVI IKAAKGAKAW EKAEDPIYAL EHSLPIDVQH YLEHHLSQPL LRIFEPVMKN
     PKELLCGDHT RCIAVSTPST AAGGIMRFAK VTMSCLACKA PLPSGCKDSL CGHCKPQEAE
     IYSRTLGTVS ELEAQYGALW TACQRCQGSL HMDVLCTSRD CPIFYRRKKV QKDLNEAMGQ
     LDRFDATSW
//
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