ID A0A2K3M2F5_TRIPR Unreviewed; 108 AA.
AC A0A2K3M2F5;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Nicotianamine synthase {ECO:0000256|RuleBase:RU368095};
DE EC=2.5.1.43 {ECO:0000256|RuleBase:RU368095};
GN ORFNames=L195_g041041 {ECO:0000313|EMBL:PNX84976.1};
OS Trifolium pratense (Red clover).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Trifolium.
OX NCBI_TaxID=57577 {ECO:0000313|EMBL:PNX84976.1, ECO:0000313|Proteomes:UP000236291};
RN [1] {ECO:0000313|EMBL:PNX84976.1, ECO:0000313|Proteomes:UP000236291}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Tatra {ECO:0000313|Proteomes:UP000236291};
RC TISSUE=Young leaves {ECO:0000313|EMBL:PNX84976.1};
RX PubMed=24500806; DOI=10.3732/ajb.1300340;
RA Istvanek J., Jaros M., Krenek A., Repkova J.;
RT "Genome assembly and annotation for red clover (Trifolium pratense;
RT Fabaceae).";
RL Am. J. Bot. 101:327-337(2014).
RN [2] {ECO:0000313|EMBL:PNX84976.1, ECO:0000313|Proteomes:UP000236291}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Tatra {ECO:0000313|Proteomes:UP000236291};
RC TISSUE=Young leaves {ECO:0000313|EMBL:PNX84976.1};
RX PubMed=28382043; DOI=10.3389/fpls.2017.00367;
RA Istvanek J., Dluhosova J., Dluhos P., Patkova L., Nedelnik J., Repkova J.;
RT "Gene Classification and Mining of Molecular Markers Useful in Red Clover
RT (Trifolium pratense) Breeding.";
RL Front. Plant Sci. 8:367-367(2017).
CC -!- FUNCTION: Synthesizes nicotianamine, a polyamine which serves as a
CC sensor for the physiological iron status within the plant, and/or might
CC be involved in the transport of iron. {ECO:0000256|RuleBase:RU368095}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC Evidence={ECO:0000256|RuleBase:RU368095};
CC -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC {ECO:0000256|ARBA:ARBA00007009, ECO:0000256|RuleBase:RU368095}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PNX84976.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; ASHM01047664; PNX84976.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K3M2F5; -.
DR EnsemblPlants; Tp57577_TGAC_v2_mRNA28190; Tp57577_TGAC_v2_mRNA28190; Tp57577_TGAC_v2_gene27254.
DR Gramene; Tp57577_TGAC_v2_mRNA28190; Tp57577_TGAC_v2_mRNA28190; Tp57577_TGAC_v2_gene27254.
DR Proteomes; UP000236291; Unassembled WGS sequence.
DR GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR004298; Nicotian_synth.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR32266:SF24; NICOTIANAMINE SYNTHASE; 1.
DR PANTHER; PTHR32266; NICOTIANAMINE SYNTHASE 3; 1.
DR Pfam; PF03059; NAS; 1.
DR PROSITE; PS51142; NAS; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000236291};
KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU368095};
KW Transferase {ECO:0000256|RuleBase:RU368095}.
SQ SEQUENCE 108 AA; 12094 MW; D362B7EE7F804647 CRC64;
MDKKEKAKVI NHLAKYMAPN AILVLRSAHG ARAFLYPIVD PCFDLKGFEV LSIFHPIDEV
INSVIVARKC LVNQQGINYL KNKCCEVEGF NPLNHGNVVD ELSIEDQA
//
