ID A0A2K5CCV2_AOTNA Unreviewed; 1024 AA.
AC A0A2K5CCV2;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 28-MAR-2018, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=Thyrotropin releasing hormone degrading enzyme {ECO:0000313|Ensembl:ENSANAP00000006662.1};
GN Name=TRHDE {ECO:0000313|Ensembl:ENSANAP00000006662.1};
OS Aotus nancymaae (Ma's night monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Platyrrhini; Aotidae;
OC Aotus.
OX NCBI_TaxID=37293 {ECO:0000313|Ensembl:ENSANAP00000006662.1, ECO:0000313|Proteomes:UP000233020};
RN [1] {ECO:0000313|Ensembl:ENSANAP00000006662.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR634016-3};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3};
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136}.
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DR AlphaFoldDB; A0A2K5CCV2; -.
DR STRING; 37293.ENSANAP00000006662; -.
DR Ensembl; ENSANAT00000024432.1; ENSANAP00000006662.1; ENSANAG00000021382.1.
DR GeneTree; ENSGT00940000155878; -.
DR OMA; WGTMEHP; -.
DR Proteomes; UP000233020; Unplaced.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProt.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09601; M1_APN-Q_like; 1.
DR Gene3D; 1.25.50.20; -; 1.
DR Gene3D; 2.60.40.1910; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR034016; M1_APN-typ.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR PANTHER; PTHR11533:SF40; THYROTROPIN-RELEASING HORMONE-DEGRADING ECTOENZYME; 1.
DR Pfam; PF11838; ERAP1_C; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR Pfam; PF17900; Peptidase_M1_N; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR634016-3};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000233020};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT TRANSMEM 40..63
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 146..331
FT /note="Aminopeptidase N-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF17900"
FT DOMAIN 368..591
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 678..1001
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
FT REGION 76..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 441
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT BINDING 440
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 444
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 463
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT SITE 527
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ SEQUENCE 1024 AA; 116773 MW; C925BF698DA53DE9 CRC64;
MGEDDAALRA GGRGLSDPWA DSVGVRPRTT ERHIAVHKRL VLAFAVSLVA LLAVTMLAVL
LSLRFDECGA NAPPGADGGP AGFPERGGNG SLPGSARRNH HAGGDSLQPE AGGVASPGTP
SAQPPSEEER EPWQPWTQLR LSGHLKPLHY NLMLTAFMEN FTFSGEVNVE IACRNATRYV
VLHASRVAVE KVQLAEDRAF GAVPVAGFFL YPQTQVLVVV LNRTLDAQRN YNLKIIYNAL
IENELLGFFR SSYVLHGERR FLGVTQFSPT HARKAFPCFD EPIYKATFKI SIKHQATYLS
LSNMPVETSV FEEDGWVTDH FSQTPLMSTY YLAWAICNFT YRETTTKSGV VVRLYARPDA
IRRGSGDYAL HITKRLIEFY EDYFKVPYSL PKLDLLAVPK HPYAAMENWG LSIFVEQRIL
LDPSVSSISY LLDVTMVIVH EICHQWFGDL VTPVWWEDVW LKEGFAHYFE FVGTDYLYPG
WNMEKQRFLT DVLHEVMLLD GLASSHPVSQ EVLQATDIDR VFDWIAYKKG AALIRMLANF
MGHSVFQRGL QDYLTIHKYG NAARNDLWNT LSEALKRTGK YVNIQEVMDQ WTLQMGYPVI
TILGNTTAEN RIVITQQHFI YDISAKTKAL KLQNNSYLWQ IPLTIVVGNR SHVSSEAIIW
VSNKSEHHRI TYLDKGSWLL GNINQTGYFR VNYDLRNWRL LIDQLIRNHE VLSVSNRAGL
IDDAFSLARA GYLPQNIPLE IIRYLSEEKD FLPWHAASRA LYPLDKLLDR MENYNVFNEY
ILKQVAATYI KLGWPKNNFN GSLVQASYQH EELRREVIML ACSFGNKHCH QQASTLISDW
ISSNRNRIPL NVRDIVYCTG VSLLDEDVWE FIWMKFHSTT AVSEKKILLE ALTCSDDRNL
LNRLLNLSLN SEVVLDQDAI DVIIHVARNP HGRDLAWKFF RDKWKILSTR YGEALFMNSK
LISGVTEFLN TEGELKELKN FMKSYDGVAA ASFSRAVETV EANVRWKMLY QDELFQWLGK
ALRH
//
