ID A0A2K5VQ02_MACFA Unreviewed; 1165 AA.
AC A0A2K5VQ02;
DT 28-MAR-2018, integrated into UniProtKB/TrEMBL.
DT 02-JUN-2021, sequence version 2.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN Name=DHX8 {ECO:0000313|Ensembl:ENSMFAP00000026839.2};
OS Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9541 {ECO:0000313|Ensembl:ENSMFAP00000026839.2, ECO:0000313|Proteomes:UP000233100};
RN [1] {ECO:0000313|Ensembl:ENSMFAP00000026839.2, ECO:0000313|Proteomes:UP000233100}
RP NUCLEOTIDE SEQUENCE.
RA Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSMFAP00000026839.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR AlphaFoldDB; A0A2K5VQ02; -.
DR Ensembl; ENSMFAT00000001021.2; ENSMFAP00000026839.2; ENSMFAG00000044651.2.
DR VEuPathDB; HostDB:ENSMFAG00000044651; -.
DR GeneTree; ENSGT00940000155510; -.
DR Proteomes; UP000233100; Chromosome 16.
DR Bgee; ENSMFAG00000044651; Expressed in lymph node and 13 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0016604; C:nuclear body; IEA:Ensembl.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; IEA:Ensembl.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IEA:Ensembl.
DR CDD; cd17971; DEXHc_DHX8; 1.
DR CDD; cd21691; GH2-like_DHX8; 1.
DR CDD; cd05684; S1_DHX8_helicase; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044762; DHX8/Prp22_DEXHc.
DR InterPro; IPR049588; DHX8_GH2-like.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049621; S1_DHX8_helicase.
DR InterPro; IPR003029; S1_domain.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF85; ATP-DEPENDENT RNA HELICASE DHX8; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50126; S1; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000233100}.
FT DOMAIN 210..281
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
FT DOMAIN 520..683
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 701..881
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 99..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 280..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 123..139
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 140..171
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 172..205
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 282..312
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1165 AA; 133202 MW; 040340B8AB40CD8D CRC64;
MAVAVAMAGA IVGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN
TTFDTFKASL VKNGTMLDED DVKVAVDVLK ELEALMPSAA VQEKQRDTEH RDRTKKKKRS
RSRDRDRDRD RDRDRERNRE RDHKRRHRSR SRSRSRTRER NKAKSRYRSR SRSQSPPKDR
KDRDKYGERN LDRWRDKHVD RPPPEEPTIG DIYNGKVTSI MQFGCFVQLE GLRKRWEGLV
HISELRREGR VANVADVVSK GQRVKVKVLS FTGTKTSLSM KDVDQESGED LNPNRRRNLV
GETNEETSMR NPDRPTHLSL VSAPEVEDDS LERKRLTRIS DPEKWEIKQM IAANVLSKEE
FPDFDEETGI LPKVDDEEDE DLEIELVEEE PPFLRGHTKQ SMDMSPIKIV KNPDGSLSQA
AMMQSALAKE RRELKQAQRE AEMDSIPMGL NKHWVDPLPD AEGRQIAANM RGIGMMPNDI
PEWKKHAFGG NKASYGKKTQ MSILEQRESL PIYKLKEQLV QAVHDNQILI VIGETGSGKT
TQITQYLAEA GYTSRGKIGC TQPRRVAAMS VAKRVSEEFG CCLGQEVGYT IRFEDCTSPE
TVIKYMTDGM LLRECLIDPD LTQYAIIMLD EAHERTIHTD VLFGLLKKTV QKRQDMKLIV
TSATLDAVKF SQYFYEAPIF TIPGRTYPVE ILYTKEPETD YLDASLITVM QIHLTEPPGD
ILVFLTGQEE IDTACEILYE RMKSLGPDVP ELIILPVYSA LPSEMQTRIF DPAPPGSRKV
VIATNIAETS LTIDGIYYVV DPGFVKQKVY NSKTGIDQLV VTPISQAQAK QRAGRAGRTG
PGKCYRLYTE RAYRDEMLTT NVPEIQRTNL ASTVLSLKAM GINDLLSFDF MDAPPMETLI
TAMEQLYTLG ALDDEGLLTR LGRRMAEFPL EPMLCKMLIM SVHLGCSEEM LTIVSMLSVQ
NVFYRPKDKQ ALADQKKAKF HQTEGDHLTL LAVYNSWKNN KFSNPWCYEN FIQARSLRRA
QDIRKQMLGI MDRHKLDVVS CGKSTVRVQK AICSGFFRNA AKKDPQEGYR TLIDQQVVYI
HPSSALFNRQ PEWVVYHELV LTTKEYMREV TTIDPRWLVE FAPAFFKVSD PTKLSKQKKQ
QRLEPLYNRY EEPNAWRISR AFRRR
//
