ID A0A2K8KLN6_9GAMM Unreviewed; 497 AA.
AC A0A2K8KLN6;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000256|HAMAP-Rule:MF_00945};
GN Name=nusA {ECO:0000256|HAMAP-Rule:MF_00945,
GN ECO:0000313|EMBL:ATX75652.1};
GN ORFNames=REIFOR_00482 {ECO:0000313|EMBL:ATX75652.1};
OS Reinekea forsetii.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Saccharospirillaceae; Reinekea.
OX NCBI_TaxID=1336806 {ECO:0000313|EMBL:ATX75652.1, ECO:0000313|Proteomes:UP000229757};
RN [1] {ECO:0000313|EMBL:ATX75652.1, ECO:0000313|Proteomes:UP000229757}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hel1_31_D35 {ECO:0000313|EMBL:ATX75652.1,
RC ECO:0000313|Proteomes:UP000229757};
RX PubMed=28000419;
RA Avci B., Hahnke R.L., Chafee M., Fischer T., Gruber-Vodicka H.,
RA Tegetmeyer H.E., Harder J., Fuchs B.M., Amann R.I., Teeling H.;
RT "Genomic and physiological analyses of 'Reinekea forsetii' reveal a
RT versatile opportunistic lifestyle during spring algae blooms.";
RL Environ. Microbiol. 19:1209-1221(2017).
CC -!- FUNCTION: Participates in both transcription termination and
CC antitermination. {ECO:0000256|HAMAP-Rule:MF_00945}.
CC -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC dependent RNA polymerase and to nascent RNA. {ECO:0000256|HAMAP-
CC Rule:MF_00945}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00945}.
CC -!- SIMILARITY: Belongs to the NusA family. {ECO:0000256|HAMAP-
CC Rule:MF_00945}.
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DR EMBL; CP011797; ATX75652.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K8KLN6; -.
DR KEGG; rfo:REIFOR_00482; -.
DR OrthoDB; 9807233at2; -.
DR Proteomes; UP000229757; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006353; P:DNA-templated transcription termination; IEA:UniProtKB-UniRule.
DR GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR CDD; cd02134; KH-II_NusA_rpt1; 1.
DR CDD; cd22529; KH-II_NusA_rpt2; 1.
DR CDD; cd04455; S1_NusA; 1.
DR Gene3D; 3.30.300.20; -; 2.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.30.1480.10; NusA, N-terminal domain; 1.
DR HAMAP; MF_00945_B; NusA_B; 1.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR025249; KH_dom_NusA-like.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR030842; NusA_bac.
DR InterPro; IPR036555; NusA_N_sf.
DR InterPro; IPR003029; S1_domain.
DR InterPro; IPR013735; TF_NusA_N.
DR InterPro; IPR010214; Tscrpt_termin_fac_NusA_C_rpt.
DR InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR NCBIfam; TIGR01953; NusA; 1.
DR NCBIfam; TIGR01954; nusA_Cterm_rpt; 2.
DR PANTHER; PTHR22648; TRANSCRIPTION TERMINATION FACTOR NUSA; 1.
DR PANTHER; PTHR22648:SF0; TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA; 1.
DR Pfam; PF14520; HHH_5; 1.
DR Pfam; PF13184; KH_5; 1.
DR Pfam; PF08529; NusA_N; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00322; KH; 2.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF54814; Prokaryotic type KH domain (KH-domain type II); 2.
DR SUPFAM; SSF47794; Rad51 N-terminal domain-like; 2.
DR SUPFAM; SSF69705; Transcription factor NusA, N-terminal domain; 1.
DR PROSITE; PS50084; KH_TYPE_1; 1.
DR PROSITE; PS50126; S1; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00945};
KW Reference proteome {ECO:0000313|Proteomes:UP000229757};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00945};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00945};
KW Transcription antitermination {ECO:0000256|ARBA:ARBA00022814,
KW ECO:0000256|HAMAP-Rule:MF_00945};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00945};
KW Transcription termination {ECO:0000256|ARBA:ARBA00022472,
KW ECO:0000256|HAMAP-Rule:MF_00945}.
FT DOMAIN 139..204
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
SQ SEQUENCE 497 AA; 55162 MW; 357AC78A08C08E5C CRC64;
MSKEILLVVD AVANEKGVSR EVIFDAMELA LATAAKKRLN DEGEPDIYVV IDRKTGEYET
FRRWLVMDNS GVPTLGSEWT TQEAHEVDLT LQPGDVYQEK IEGEKFGRIS AQAAKQIIVQ
KVRDAERARI VDAYRDRIGE MVSGTVKKVS REGLILDLGN NAEAVLPREQ MIGRENFRMS
DRVRAVLHSI KPEGRGPQLE LSRSSNHMLI ELFRIEVPEI QEELLDIRAC ARDPGLRAKI
AVKTNDKRID PIGACVGMRG SRVQAVSNEL GNERVDIILW DDNPAQLVIN AMAPAEVASI
VMDEETNSMQ VAVAAENLAM AIGRSGQNVR LASELTGWTI NVMTEDEAGA RQEQEVGQLV
SHFVSKMDID EEIAIALVEE GFTTLEEVAY VPLSEMLEID GFDEDLVNEL RQRAKDILLT
QELASEEELE SAQPHADLLA LEGMNEHLAL VLASKGIVSM EDVAELSIDE FMEATGLEEE
QSGKLIMAAR APWFENE
//
