ID A0A2K8L9P3_9PROT Unreviewed; 549 AA.
AC A0A2K8L9P3;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE SubName: Full=B12 binding domain-containing protein {ECO:0000313|EMBL:ATX82969.1};
GN ORFNames=Ga0123462_2134 {ECO:0000313|EMBL:ATX82969.1};
OS Mariprofundus ferrinatatus.
OC Bacteria; Pseudomonadota; Zetaproteobacteria; Mariprofundales;
OC Mariprofundaceae; Mariprofundus.
OX NCBI_TaxID=1921087 {ECO:0000313|EMBL:ATX82969.1, ECO:0000313|Proteomes:UP000231637};
RN [1] {ECO:0000313|EMBL:ATX82969.1, ECO:0000313|Proteomes:UP000231637}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CP-8 {ECO:0000313|EMBL:ATX82969.1,
RC ECO:0000313|Proteomes:UP000231637};
RA McAllister S.M., Kato S., Chan C.S., Chiu B.K., Field E.K.;
RT "Isolation and genomic insights into novel planktonic Zetaproteobacteria
RT from stratified waters of the Chesapeake Bay.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CP018800; ATX82969.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K8L9P3; -.
DR KEGG; mfn:Ga0123462_2134; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000231637; Chromosome.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000231637}.
FT DOMAIN 16..174
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 224..450
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 549 AA; 61216 MW; F933388786F3669B CRC64;
MPKQVNVLFV IPPYFNLDDF ISKDRTNVLP AFTMPYGVLS LDAYIKARCE ASVETAILDL
NVPLAQILGQ PGVSQDQVLA MLNNEVAKAL TCFQADIVGV SALFNSSFRY IEDIATVVKQ
ALPNVLVTAG GGLPSAAFDR MLGYCPSIDA VCKGEGEIPL ADLIDTDDRS ALLESHQSWI
TRSGLAVGKQ PGHTFVQNLD DIPPLDYRTI DLNHYNSRSI DKRYIGMKKR EMAIHTSRGC
PFLCVFCSNP SLHGRAVRTM SVDRVEAEVK RMRDEFGLTV LMIEDDHFFH DKERAKDVLR
RLIDLDIRIE FPNGVAVYAI DDEVAELFAK AGVSTVALAV ESGSDYVLDH LIKKPLKKKL
IKPAVEALRR HGVQSHVFIV IGLPGEMDEH RQETLEMMID SGFDWAHIYC AIPIFGSRLY
DICADNGYIE NSNPLDFINT KSVIRAPGVD PDAIADIAYH MNLVANFVEN HNMKIENYST
AIKYFKNVCE KYPDHAFGQY FLGMACKLSG MDLASADKCM KKFNEIVASD EWWRSKAVKY
GLLKSPVEG
//
