ID A0A2K9E5I6_9FIRM Unreviewed; 1681 AA.
AC A0A2K9E5I6;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE SubName: Full=tRNA nuclease WapA {ECO:0000313|EMBL:AUG56726.1};
DE EC=3.1.-.- {ECO:0000313|EMBL:AUG56726.1};
GN Name=wapA8 {ECO:0000313|EMBL:AUG56726.1};
GN ORFNames=HVS_03905 {ECO:0000313|EMBL:AUG56726.1};
OS Acetivibrio saccincola.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=1677857 {ECO:0000313|EMBL:AUG56726.1, ECO:0000313|Proteomes:UP000233534};
RN [1] {ECO:0000313|EMBL:AUG56726.1, ECO:0000313|Proteomes:UP000233534}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SR1 {ECO:0000313|Proteomes:UP000233534};
RA Pechtl A., Ruckert C., Koeck D.E., Maus I., Winkler A., Kalinowski J.,
RA Puhler A., Schwarz W.W., Zverlov V.V., Schluter A., Liebl W.;
RT "Complete genome sequence of Herbivorax saccincola GGR1, a novel
RT Cellulosome-producing hydrolytic bacterium in a thermophilic biogas plant,
RT established by Illumina and Nanopore MinION sequencing.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; CP025197; AUG56726.1; -; Genomic_DNA.
DR KEGG; hsc:HVS_03905; -.
DR Proteomes; UP000233534; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd12871; Bacuni_01323_like; 1.
DR CDD; cd00081; Hint; 1.
DR CDD; cd04859; Prim_Pol; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 1.
DR Gene3D; 2.180.10.10; RHS repeat-associated core; 3.
DR InterPro; IPR015330; DNA_primase/pol_bifunc_N.
DR InterPro; IPR006500; Helicase_put_C_phage/plasmid.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR014818; Phage/plasmid_primase_P4_C.
DR InterPro; IPR014820; PriCT_1.
DR InterPro; IPR022385; Rhs_assc_core.
DR InterPro; IPR031325; RHS_repeat.
DR InterPro; IPR006530; YD.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01613; primase_Cterm; 1.
DR NCBIfam; TIGR03696; Rhs_assc_core; 1.
DR NCBIfam; TIGR01643; YD_repeat_2x; 11.
DR PANTHER; PTHR32305; -; 1.
DR PANTHER; PTHR32305:SF15; PROTEIN RHSA-RELATED; 1.
DR Pfam; PF08706; D5_N; 1.
DR Pfam; PF08708; PriCT_1; 1.
DR Pfam; PF09250; Prim-Pol; 1.
DR Pfam; PF07591; PT-HINT; 1.
DR Pfam; PF05593; RHS_repeat; 11.
DR SMART; SM00885; D5_N; 1.
DR SMART; SM00306; HintN; 1.
DR SMART; SM00942; PriCT_1; 1.
DR SMART; SM00943; Prim-Pol; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF56747; Prim-pol domain; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000313|EMBL:AUG56726.1};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000233534};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 1322..1348
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1393..1411
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1431..1452
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1562..1586
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
SQ SEQUENCE 1681 AA; 188837 MW; A5E11B627816CB94 CRC64;
MKMTMMDAAL KYAEANIPVI PLHWICEDGS CSCKAGRNCD SKGKHPLYTG WYKNSTSDIE
QIRKWWTKTP NANIGIPTGE KSGWLVLDVD DGGDETLSAL EATHGKLPDT VTAVTGRGGR
YYIFKYPKGR SIPNKTKFAP GLDTRSTGGL IVVAPSIHVS GNRYEWIKDH SPFDRTLAEA
PEWLLKLMER VEVLLTSFEG SSIAAEIKEG SRNSTLTSLA GTMRARGMTE ESIYAALLAE
NNARCNPPLD EAKVKKIAHS VSRYQPNPPM KKHYHRTDSG NAERLRDRFG SIIRYCPAFK
YWLVYDGCCW KRETGELMQF AIKTARDMLA EASRIEDEAM RKELVRHAMQ SENAGRLKAM
IDVASNLEGL VIMPDELDAE IWKLNCKNGV VNLKTGELLP HKREYYMSKI CPVEYKPSSK
APRWMDFLNT ITGGSKELVR YLQKAVGSSL SGDISEQALF VLYGTGANGK STFLNTISEL
LGDYARNTPS ETFMAKRIEA IGNDIASKET VYKYDSRGNL ISETDPEGNT KSYKYDSMGN
KISETDANGN EVLYKYNSNG CMTSKTEIDK VTGKEIKTEY IYDSMNRLIG MIDGNGKSIR
IEYNAAGQQS ALIDKTGNRT EYEYDAFGNL ILVRYADGTT EKSTYDAEGR ETSSTDRLGR
TTRYEYDKLG RLIKTINPDG SCVENEYDPL GRIISVKDER GNVTKYKYDE VGNTTEVIDA
LGNVTKYEYD KNGNRTKMID ANGNVITFEY DSDNNPVRTI FPDGTYTSYE YNSIGQKISE
RDQAGLITVY EYDAAGRVTK VIDPLGNETC FEYDAKGNRI SQTDANGNKV RFEYDKIGNV
TKQILPLGYE EVITYDDEGK VTSKTDFNGS KIEFEYDVDG NLIKKTYPDG KSEVYTYTLS
GQRESVTDER GTTYYEYDLM DRLIKQINPD GTQITYTYDK AGNCTSVTVP SGTTYYTYDE
LNRLKSVTDP DGNTTTYTYD AIGNRKSVTY PNKTTTEYEY DYLSRLISLL NRNEAGEIIS
SYKYTLGPAG NRIRTEENNG RVIKYTYDDT YKLIKEEIEN PDGDKTIIEY TYDAVGNRLS
KSVNGVVTEY TYDENNRLIT EGQTVYTYDK NGNTLSKKTN TGEEVTYTYD YNNRLTKVNI
TGAKDASTVE YAYDVDGIRV QKVVDGTNIT NYLVDENRLY AQVLEERDDE GNLTVSYVYG
DDLISQKRGD SFSYYHYDGI GSTRALTDIH GNITDTYTYD SFGMLTSRTG TTENDYLFTG
EQYDANVNFY YLRARYMNPA LGRFLTMDSW GGIITDPITL HKYLYADCDP VNKIDPSGHF
SILGTLALVT YTVSIAAIAL PVFAGIYLTI VNKVSVLDYI SALCSEDVWI EAAIGIGMGA
VLGLGIKAIA KKLACEVVAF IGVIMSLWSL YQSIDLSINM IKGGVSREQV ARYLAVLTAT
IILTTLIGKV CFTEDTLIKT EDGYKEIKDI EVGDLVYSED PLTGEKGLKR VTNIFVNETS
VLVRIYVEDE EIETTPTHPF WVIGKGWVAA GDIEAGDKVY LYSGEGREVK EVRFEYLDTP
IKVYNFEVED WHTYFVSEQD VFVHNSCGDD MLGSKGSQTS SKTTWSRGKT ERIDVENPAP
GKRPGQIHYH EPNNTKWYYD IDNNQFYDQK TGNLAPNKIQ KLLKDSSFIQ GIKKALKVLG
V
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