ID A0A2K9L9A8_9RHOO Unreviewed; 654 AA.
AC A0A2K9L9A8;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Nitrous-oxide reductase {ECO:0000256|ARBA:ARBA00016560};
DE EC=1.7.2.4 {ECO:0000256|ARBA:ARBA00011896};
DE AltName: Full=N(2)OR {ECO:0000256|ARBA:ARBA00031077};
DE AltName: Full=N2O reductase {ECO:0000256|ARBA:ARBA00032847};
GN ORFNames=B4966_05785 {ECO:0000313|EMBL:AUL99723.1};
OS Rhodocyclaceae bacterium.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
OC Rhodocyclaceae.
OX NCBI_TaxID=1898103 {ECO:0000313|EMBL:AUL99723.1, ECO:0000313|Proteomes:UP000235024};
RN [1] {ECO:0000313|EMBL:AUL99723.1, ECO:0000313|Proteomes:UP000235024}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thauera-like {ECO:0000313|EMBL:AUL99723.1,
RC ECO:0000313|Proteomes:UP000235024};
RA Umu S.U., Elley S., Stott M., Poole A.M.;
RT "A full genome of an Rhodocyclaceae isolate from Taupo Volcanic zone.";
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Nitrous-oxide reductase is part of a bacterial respiratory
CC system which is activated under anaerobic conditions in the presence of
CC nitrate or nitrous oxide. {ECO:0000256|ARBA:ARBA00003034}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 Fe(III)-[cytochrome c] + H2O + N2 = 2 Fe(II)-[cytochrome c]
CC + 2 H(+) + nitrous oxide; Xref=Rhea:RHEA:43108, Rhea:RHEA-COMP:10350,
CC Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17045, ChEBI:CHEBI:17997, ChEBI:CHEBI:29033,
CC ChEBI:CHEBI:29034; EC=1.7.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00029370};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- COFACTOR:
CC Name=Cu cation; Xref=ChEBI:CHEBI:23378;
CC Evidence={ECO:0000256|ARBA:ARBA00001935};
CC -!- PATHWAY: Nitrogen metabolism; nitrate reduction (denitrification);
CC dinitrogen from nitrate: step 4/4. {ECO:0000256|ARBA:ARBA00004779}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000256|ARBA:ARBA00004418}.
CC -!- SIMILARITY: Belongs to the NosZ family.
CC {ECO:0000256|ARBA:ARBA00010372}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the cytochrome c
CC oxidase subunit 2 family. {ECO:0000256|ARBA:ARBA00006790}.
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DR EMBL; CP020026; AUL99723.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K9L9A8; -.
DR KEGG; rbh:B4966_05785; -.
DR OrthoDB; 9759695at2; -.
DR UniPathway; UPA00652; UER00709.
DR Proteomes; UP000235024; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR GO; GO:0050304; F:nitrous-oxide reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0019333; P:denitrification pathway; IEA:UniProtKB-UniPathway.
DR CDD; cd04223; N2OR_C; 1.
DR Gene3D; 2.60.40.420; Cupredoxins - blue copper proteins; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR002429; CcO_II-like_C.
DR InterPro; IPR008972; Cupredoxin.
DR InterPro; IPR028096; EfeO_Cupredoxin.
DR InterPro; IPR011045; N2O_reductase_N.
DR InterPro; IPR034205; N2OR_C.
DR InterPro; IPR023644; NO_Rdtase.
DR InterPro; IPR041114; Nos_propeller.
DR InterPro; IPR041142; NOS_propeller_2.
DR InterPro; IPR006311; TAT_signal.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR NCBIfam; TIGR04244; nitrous_NosZ_RR; 1.
DR PANTHER; PTHR42838; CYTOCHROME C OXIDASE SUBUNIT II; 1.
DR PANTHER; PTHR42838:SF1; NITROUS-OXIDE REDUCTASE; 1.
DR Pfam; PF13473; Cupredoxin_1; 1.
DR Pfam; PF18764; nos_propeller; 1.
DR Pfam; PF18793; nos_propeller_2; 1.
DR SUPFAM; SSF49503; Cupredoxins; 1.
DR SUPFAM; SSF50974; Nitrous oxide reductase, N-terminal domain; 1.
DR PROSITE; PS50857; COX2_CUA; 1.
DR PROSITE; PS51318; TAT; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Periplasm {ECO:0000256|ARBA:ARBA00022764};
KW Reference proteome {ECO:0000313|Proteomes:UP000235024};
KW Signal {ECO:0000256|ARBA:ARBA00022729}.
FT DOMAIN 558..654
FT /note="Cytochrome oxidase subunit II copper A binding"
FT /evidence="ECO:0000259|PROSITE:PS50857"
SQ SEQUENCE 654 AA; 72020 MW; 1AB9F0A9E67A3C68 CRC64;
MKHDQTNNTL DAQPDPGRRK FLNTAAFAGL AGAGLSVGLS ACKQEQAAAP AAAPAAPAAA
PAAKTASNGS HKKVHLAPGE LDTYYGLWSG GHTGDFRVLG LPSGRELHRV PCFVPDALVG
WGITNESKAV MGTKPDGTLK YTVGDTHHVH ASYKDGNYDG RYAWINDKIN SRLARIRLDY
FVCDKITEIP NIQGFHGIFP DKRDPVDPSI NYTTRVFCGA EFHLPMPNDG RDLESPEKYR
ALFTCVDAES MEVRWQVLID GNCDLVATSF DGKLAASNQY NTEGGYRYEG MMSAERDACI
FFNIARIEQA VKDGKFITIG DSKVPVVDGT RAANADPKTA LVAYVSVPKN PHGVNASPDG
KYFICAGKLS PTATVIELAK VLDWFDGKLD ELDKAIVAEV ELGLGPLHTA FDGRGNAYTT
LFLDSQIVKW NIDAAIKFHA GDTSVQYVVD RLDVHYQPGH INATQSETMF ADGQWLMVGC
KFSKDRFLPV GPLHAETEQL VDISGEKMVL VADHPVRPEP HDFIMFKREL LRPKQVYDLN
DFPLAIKDPK EGGVTRNGNK VTVRITSQAP AFDPREFKVK RGDEVTIILT NLDKIEDLTH
GFAIPKYNIN FIVNPQETAS VTFKADKPGV YWYYCTHFCH ALHLEMRGRM IVEA
//
