ID A0A2L0D6L8_9STRE Unreviewed; 878 AA.
AC A0A2L0D6L8;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460,
GN ECO:0000313|EMBL:AUW97493.1};
GN ORFNames=C0J00_00980 {ECO:0000313|EMBL:AUW97493.1};
OS Streptococcus pluranimalium.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=82348 {ECO:0000313|EMBL:AUW97493.1, ECO:0000313|Proteomes:UP000238956};
RN [1] {ECO:0000313|EMBL:AUW97493.1, ECO:0000313|Proteomes:UP000238956}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TH11417 {ECO:0000313|EMBL:AUW97493.1,
RC ECO:0000313|Proteomes:UP000238956};
RA Hurst M.R.H.;
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AUW97493.1, ECO:0000313|Proteomes:UP000238956}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TH11417 {ECO:0000313|EMBL:AUW97493.1,
RC ECO:0000313|Proteomes:UP000238956};
RA Zhang J.-R., Hu G.-Z.;
RT "Whole genome sequencing analysis of Streptococcus pluranimalium isolated
RT from cattle infected mastitis in China.";
RL Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541, ECO:0000256|RuleBase:RU004460}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; CP025536; AUW97493.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2L0D6L8; -.
DR KEGG; splr:C0J00_00980; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000238956; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000238956};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 6..269
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 299..466
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 634..842
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 878 AA; 99266 MW; 915F2DCBE2B39634 CRC64;
MTNAKNKLLL IDGSSVAFRA FFALYNQIDR FKNASGLHTN AIYGFHLMLD HLMERVQPTH
ILVAFDAGKT TFRTEMYADY KAGRAKTPDE FREQFPYIRD MLAARGVPFY DLDNYEADDI
IGTLDKMAEL DDFDVTIVSG DKDLIQLTDQ NTVVEISKKG VAEFEEFTPA YLMEKMGITP
EQFIDLKALM GDKSDNIPGV TKIGEKTGLK LLLEHGSLEG IYNNIDGMKQ SKMKENLIKD
KELAFLSKTL ATINTKAPIT IDLSDTVYMG PKVEELAKFY DEMGFTQFKN ALVGQAETPT
FDTPFEEVST VTDKHFSDQD FFYFEMLNDN YHVEEIIGFA WGTKETIYAS TNLDLLQEPA
FQEALAKPMQ TFDFKRVKVL LSHHGIDIPA PRFDSRLAKY LLSTVEDNQL STIARLYTAL
PLENDEVVYG KGAKRAVPEK EVLLGHLAKK VQVLIESKEP MLAKLEENDQ IDLLFDMEQP
LANVLAKMEI AGIKVNGGTL AEMALENQVS IDKLTQEIYE IAGQEFNINS PKQLGVLLFE
TMGLPLEYTK KTKTGYSTAV DVLERLAPIS PIVSKILEYR QITKLQSTYL IGLQEYIRDD
GKIHTRYVQD LTQTGRLSST DPNLQNIPIR LEQGRLIRKA FTPSTDKAVL LSSDYSQIEL
RVLAHISGDE HLIDAFKHGA DIHTSTAMRV FGIEKPEDVT SNDRRNAKAV NFGIVYGISD
FGLSNNLGIP RKLAKEYIET YFERYPGIKN YMETVVREAK DKGYVETLFK RRRELPDINS
RNFNVRHFAE RTAINSPIQG SAADILKIAM INLDKALIDG NFKTKMLLQV HDEIVLEVPE
DEIPAIQKLV KEVMEAAVEM TVPLLAEESY GQTWYEAK
//
