ID   A0A2L2T3F1_9HYPO        Unreviewed;       556 AA.
AC   A0A2L2T3F1;
DT   25-APR-2018, integrated into UniProtKB/TrEMBL.
DT   25-APR-2018, sequence version 1.
DT   27-MAR-2024, entry version 17.
DE   RecName: Full=Amidase domain-containing protein {ECO:0000259|Pfam:PF01425};
OS   Fusarium venenatum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX   NCBI_TaxID=56646 {ECO:0000313|EMBL:CEI39759.1, ECO:0000313|Proteomes:UP000245910};
RN   [1] {ECO:0000313|Proteomes:UP000245910}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A3/5 {ECO:0000313|Proteomes:UP000245910};
RA   King R.;
RL   Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; LN649232; CEI39759.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2L2T3F1; -.
DR   STRING; 56646.A0A2L2T3F1; -.
DR   OrthoDB; 731186at2759; -.
DR   Proteomes; UP000245910; Chromosome iiii.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.1300.10; Amidase signature (AS) domain; 1.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR46072:SF3; AMIDASE; 1.
DR   PANTHER; PTHR46072; AMIDASE-RELATED-RELATED; 1.
DR   Pfam; PF01425; Amidase; 2.
DR   PIRSF; PIRSF001221; Amidase_fungi; 1.
DR   SUPFAM; SSF75304; Amidase signature (AS) enzymes; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000245910}.
FT   DOMAIN          83..366
FT                   /note="Amidase"
FT                   /evidence="ECO:0000259|Pfam:PF01425"
FT   DOMAIN          435..538
FT                   /note="Amidase"
FT                   /evidence="ECO:0000259|Pfam:PF01425"
FT   ACT_SITE        139
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   ACT_SITE        215
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   ACT_SITE        239
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   BINDING         189
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
FT   BINDING         215
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
FT   BINDING         236..239
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
SQ   SEQUENCE   556 AA;  60823 MW;  15BB41C5F67B5AD6 CRC64;
     MPATTSWKTI AALHREKQVE AVHKEWLLPE KQLETLKEGD RLIESKAAQR SGLLSDKEIN
     ITERFTASEL LSKIHRQEIT SEEVVVAFSK RASLAQQLTS CLTEIFFEEG IERARQLDKQ
     LKETGKLAGP LHGLPISLKD SYVVKGHYAT VGYVEFLRQP IPHTNSALVD LLLDAGAVLY
     CKTNLPQTMM TADSENNVFG RALNPHRTTL TAGGSTGGEG SLIAFRGSLL GVGSDIAGSI
     RIPSLCCGIY GFKPTADRVP FGGQSEYPFR RPHLPGVTPV GGPMANSVED LELFMKITMG
     QRPWNYDPSV ADIPWRDLSG AADKRLTIGV MAEDPDYPLS PPVRRSLAKA ASALEGAGHK
     IVQIQADPKR NAGHGARIGF QYFTLVGPDP DTISRECGEP LVASVARLVH PFSKGEFPVR
     PDLELADKLF SLGEVKLEYG KAWQEVWRDN GLDVVLAPGA SSTAVPHDTY GNPVYTLMWN
     VLDFPAGIIP FGTSSKLVDD EVIKATTAFE PDYVPEETDG APCAIQIVAP RFRDEECLQA
     MQIIDRDIRL SLSQKL
//