ID A0A2M6V3C2_9BURK Unreviewed; 769 AA.
AC A0A2M6V3C2;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=NADP-dependent malic enzyme {ECO:0000313|EMBL:PIT72942.1};
GN ORFNames=B9Z42_11555 {ECO:0000313|EMBL:PIT72942.1};
OS Limnohabitans sp. B9-3.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Limnohabitans.
OX NCBI_TaxID=1100707 {ECO:0000313|EMBL:PIT72942.1, ECO:0000313|Proteomes:UP000228506};
RN [1] {ECO:0000313|EMBL:PIT72942.1, ECO:0000313|Proteomes:UP000228506}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B9-3 {ECO:0000313|EMBL:PIT72942.1,
RC ECO:0000313|Proteomes:UP000228506};
RA Kasalicky V., Mehrshad M., Andrei S.-A., Salcher M., Kratochvilova H.,
RA Simek K., Ghai R.;
RT "Unexpected and diverse lifestyles within the genus Limnohabitans.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: In the N-terminal section; belongs to the malic enzymes
CC family. {ECO:0000256|ARBA:ARBA00007686}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PIT72942.1}.
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DR EMBL; NESI01000007; PIT72942.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2M6V3C2; -.
DR OrthoDB; 9805787at2; -.
DR Proteomes; UP000228506; Unassembled WGS sequence.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10950; -; 1.
DR Gene3D; 3.40.50.10750; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR032683; Malate_DH.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR012188; ME_PTA.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR042113; P_AcTrfase_dom1.
DR InterPro; IPR042112; P_AcTrfase_dom2.
DR InterPro; IPR002505; PTA_PTB.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF12434; Malate_DH; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR Pfam; PF01515; PTA_PTB; 1.
DR PIRSF; PIRSF036684; ME_PTA; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036684-2};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|PIRSR:PIRSR036684-3};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000228506}.
FT DOMAIN 29..162
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 174..411
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
FT ACT_SITE 105
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-1"
FT BINDING 87..94
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 147
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 148
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 173
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 298
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
SQ SEQUENCE 769 AA; 83180 MW; F946737818D23FEF CRC64;
MSPTPSHNSA EARAELRRAA LEYHEFPTPG KLAIHATKQL VNQHDLALAY SPGVAAPCEE
IVKDPNNAFK YTSRGNLVAV ITNGTAVLGL GDIGPLASKP VMEGKAVLFK KFAGIDVFDL
EIDEKNLDKL VDIIASLEPT FGGINLEDIK APDCFYVERK LRERMKIPVF HDDQHGTAIT
VGAAIINGLK VAGKDIKTVK LVTSGAGAAA LACLGLLLKM GMPRSNIYVT DLAGVVYEGR
TELMDEDKIQ FAQKTDARTL SDIIEGADVF LGLSAGGVLK PDMVKKMAAK PIIMALANPN
PEITPEEVKA VRDDAIIATG RSDYHNQVNN VLCFPYIFRG ALDAGASTIT DEMEIAAVYA
IAELAQAEQS EVVAAAYAGE TLAFGPEYLI PKPFDPRLMM KIAPAVAKAA AASGVATRPI
QDMDAYIEKL QGFVYASGSI MKPIYAAAKR ASKKRVCYAE GEEERVLRAV QIVVDEGLAK
PTLIGRPAVI AQRIESFGLR LKEGVDYQVV NVEQDHRYRD FWQTYYKMTC RKGVTEQLAK
IDMRRRLSLI GTMMLHKGEV DGLICGTWST PLTHLKYVHE VIGNRQGVST YAAMNGLLLP
DRQVFLVDTH INYDPTAEQL AEITVMAAEE MRRFGIQPKA ALLSHSNFGS SDQPSAIKMR
ETLALVQKKA PWLEIDGEMH GDVALDGHAR EAQMADTTLI GDANLLVLPN LDAANIAYNL
LKTAAGGNIA IGPVLLGAAK PVHILTASTT VRRIVNMTAL TVADASAVR
//
