ID   A0A2M8LSS1_9ACTN        Unreviewed;       186 AA.
AC   A0A2M8LSS1;
DT   25-APR-2018, integrated into UniProtKB/TrEMBL.
DT   25-APR-2018, sequence version 1.
DT   28-JUN-2023, entry version 15.
DE   SubName: Full=HIT family hydrolase {ECO:0000313|EMBL:PJE95010.1};
GN   ORFNames=CUT44_25490 {ECO:0000313|EMBL:PJE95010.1};
OS   Streptomyces carminius.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=2665496 {ECO:0000313|EMBL:PJE95010.1, ECO:0000313|Proteomes:UP000230407};
RN   [1] {ECO:0000313|EMBL:PJE95010.1, ECO:0000313|Proteomes:UP000230407}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TRM SA0054 {ECO:0000313|EMBL:PJE95010.1,
RC   ECO:0000313|Proteomes:UP000230407};
RA   Wang Y., Luo X., Wan C., Zhang L.;
RT   "Streptomyces carmine sp. nov., a novel actinomycete isolated from Sophora
RT   alopecuroides in Xinjiang, China.";
RL   Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PJE95010.1}.
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DR   EMBL; PGGW01000067; PJE95010.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2M8LSS1; -.
DR   Proteomes; UP000230407; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   CDD; cd01275; FHIT; 1.
DR   Gene3D; 3.30.428.10; HIT-like; 1.
DR   InterPro; IPR039383; FHIT.
DR   InterPro; IPR011146; HIT-like.
DR   InterPro; IPR036265; HIT-like_sf.
DR   PANTHER; PTHR42997:SF1; AP-4-A PHOSPHORYLASE; 1.
DR   PANTHER; PTHR42997; HIT FAMILY HYDROLASE; 1.
DR   Pfam; PF01230; HIT; 1.
DR   SUPFAM; SSF54197; HIT-like; 1.
DR   PROSITE; PS51084; HIT_2; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000313|EMBL:PJE95010.1};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000230407}.
FT   DOMAIN          49..158
FT                   /note="HIT"
FT                   /evidence="ECO:0000259|PROSITE:PS51084"
FT   MOTIF           143..147
FT                   /note="Histidine triad motif"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00464"
FT   ACT_SITE        145
FT                   /note="Tele-AMP-histidine intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639383-1"
FT   BINDING         75
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639383-2"
FT   BINDING         147
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639383-2"
SQ   SEQUENCE   186 AA;  20009 MW;  A50C7AC952ECF225 CRC64;
     MLASMSSEPE QQIGVGSPDA FQRLWTPHRM AYIQGENKPS GPGAGDGCPF CSIPAKSDED
     GLVLARGEYV YAVLNLYPYT GGHLMVVPFR HVADYTDLTD EETAELAEHT KRAMRALRAA
     SGAQGFNIGM NQGAVAGAGI AAHLHQHIVP RWGGDTNFMP VVGHTRVLPQ LLADTRRMLA
     EAWPAG
//