ID A0A2N0I4B3_9SPHN Unreviewed; 251 AA.
AC A0A2N0I4B3;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=precorrin-2 dehydrogenase {ECO:0000256|ARBA:ARBA00012400};
DE EC=1.3.1.76 {ECO:0000256|ARBA:ARBA00012400};
GN ORFNames=B0I00_1204 {ECO:0000313|EMBL:PKB25996.1};
OS Novosphingobium kunmingense.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Novosphingobium.
OX NCBI_TaxID=1211806 {ECO:0000313|EMBL:PKB25996.1, ECO:0000313|Proteomes:UP000232587};
RN [1] {ECO:0000313|EMBL:PKB25996.1, ECO:0000313|Proteomes:UP000232587}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.12274 {ECO:0000313|EMBL:PKB25996.1,
RC ECO:0000313|Proteomes:UP000232587};
RA Whitman W.;
RT "Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the genomes of
RT soil and plant-associated and newly described type strains.";
RL Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin;
CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76;
CC Evidence={ECO:0000256|ARBA:ARBA00001156};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
CC {ECO:0000256|ARBA:ARBA00005010}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PKB25996.1}.
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DR EMBL; PHUF01000002; PKB25996.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2N0I4B3; -.
DR OrthoDB; 9815856at2; -.
DR UniPathway; UPA00262; UER00222.
DR Proteomes; UP000232587; Unassembled WGS sequence.
DR GO; GO:0004325; F:ferrochelatase activity; IEA:InterPro.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR InterPro; IPR028161; Met8-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR006367; Sirohaem_synthase_N.
DR NCBIfam; TIGR01470; cysG_Nterm; 1.
DR PANTHER; PTHR35330; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR PANTHER; PTHR35330:SF1; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR Pfam; PF13241; NAD_binding_7; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1.
DR SUPFAM; SSF53790; Tetrapyrrole methylase; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000313|EMBL:PKB25996.1};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000232587};
KW Transferase {ECO:0000313|EMBL:PKB25996.1}.
SQ SEQUENCE 251 AA; 26399 MW; F319348B72E480C8 CRC64;
MHSLPLFHRV AGQPVIVVGE GEAAAAKARL IERAGGTIVG AASKEARLAF VALDDPDEAA
AALKARGLLV NVADRPDLCD FTLPSVLDRD PVLIAVSTGG ASAGLAKVLR LRLEQMLPAS
LGRLASGLAA AREAMRRRWP EAGARRHALD AALAPGGPLD PLDQAAADRI DEWLERDSLS
ASQTIEITLA SDDPDDLTLR QARWLGSADA ILCDDNFAPQ ILARSRADAL RLPVDAAPPA
DARLTLILRT G
//
