ID   A0A2N0VGY8_9BACT        Unreviewed;       345 AA.
AC   A0A2N0VGY8;
DT   25-APR-2018, integrated into UniProtKB/TrEMBL.
DT   25-APR-2018, sequence version 1.
DT   24-JAN-2024, entry version 14.
DE   SubName: Full=Endoglucanase {ECO:0000313|EMBL:PKD43459.1};
GN   ORFNames=CWD77_07765 {ECO:0000313|EMBL:PKD43459.1};
OS   Rhodohalobacter barkolensis.
OC   Bacteria; Balneolota; Balneolia; Balneolales; Balneolaceae;
OC   Rhodohalobacter.
OX   NCBI_TaxID=2053187 {ECO:0000313|EMBL:PKD43459.1, ECO:0000313|Proteomes:UP000233398};
RN   [1] {ECO:0000313|EMBL:PKD43459.1, ECO:0000313|Proteomes:UP000233398}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=15182 {ECO:0000313|EMBL:PKD43459.1,
RC   ECO:0000313|Proteomes:UP000233398};
RA   Han S.;
RT   "Rhodohalobacter 15182 sp. nov., isolated from a salt lake.";
RL   Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001123-2};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000256|PIRSR:PIRSR001123-2};
CC   -!- SIMILARITY: Belongs to the peptidase M42 family.
CC       {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PKD43459.1}.
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DR   EMBL; PISP01000002; PKD43459.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2N0VGY8; -.
DR   OrthoDB; 9772053at2; -.
DR   Proteomes; UP000233398; Unassembled WGS sequence.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd05656; M42_Frv; 1.
DR   Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1.
DR   Gene3D; 3.40.630.10; Zn peptidases; 1.
DR   InterPro; IPR008007; Peptidase_M42.
DR   InterPro; IPR023367; Peptidase_M42_dom2.
DR   PANTHER; PTHR32481; AMINOPEPTIDASE; 1.
DR   PANTHER; PTHR32481:SF0; AMINOPEPTIDASE YPDE-RELATED; 1.
DR   Pfam; PF05343; Peptidase_M42; 1.
DR   PIRSF; PIRSF001123; PepA_GA; 1.
DR   SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000233398}.
FT   ACT_SITE        202
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-1"
FT   BINDING         63
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         172
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         172
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         203
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         225
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         314
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
SQ   SEQUENCE   345 AA;  37462 MW;  DBC0597CF6A2B5B2 CRC64;
     MKLNFKLLKE IVSTPGAPGH ESPIRSVLIN HIRDHVDEYS VDRMGNLIAR VKGDGPKVMA
     AAHMDEISLI TTHVDSKGFI RFSTLGGFDP ETLITQRVLI HGNETIPGVI GSKPIHIQTD
     SEKKSKSKLK NLFIDTGLPA DRVKELIPNG TPVTRDKDLL ELGECISSKS LDNRISVYIL
     IEALQRAKKS NCDFYAAFTV QEEVGIRGAR VAAQAIQPEI GLNLDVTLAN DLPGVSDHEV
     CTLLGEGIGI KIMDKSVICT PTLVRHLEDL ANKNSIKIQR EVLTAGGTDT SAMQYLVGIG
     SHVTSISCPV RYIHSTAETC AISDVKAGIK LTTLCIENIG SYSFK
//