ID A0A2N0ZIG6_9BACI Unreviewed; 845 AA.
AC A0A2N0ZIG6;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN ORFNames=CWS20_09315 {ECO:0000313|EMBL:PKG29284.1};
OS Cytobacillus horneckiae.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Cytobacillus.
OX NCBI_TaxID=549687 {ECO:0000313|EMBL:PKG29284.1, ECO:0000313|Proteomes:UP000233343};
RN [1] {ECO:0000313|EMBL:PKG29284.1, ECO:0000313|Proteomes:UP000233343}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1PO1SC {ECO:0000313|Proteomes:UP000233343};
RX PubMed=19666815; DOI=10.1099/ijs.0.008979-0;
RA Vaishampayan P., Probst A., Krishnamurthi S., Ghosh S., Osman S.,
RA McDowall A., Ruckmani A., Mayilraj S., Venkateswaran K.;
RT "Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean
RT room.";
RL Int. J. Syst. Evol. Microbiol. 60:1031-1037(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PKG29284.1}.
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DR EMBL; PISD01000017; PKG29284.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2N0ZIG6; -.
DR Proteomes; UP000233343; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR005738; TopoIII.
DR InterPro; IPR025589; Toprim_C_rpt.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR NCBIfam; TIGR01056; topB; 1.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF13342; Toprim_Crpt; 2.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:PKG29284.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000233343};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 1..142
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
SQ SEQUENCE 845 AA; 95951 MW; 61FB5C12F43EEFF7 CRC64;
MKLVIAEKPD AAKKMTEPYG GKLNKEKGYI EVPPCSTFPQ GAIFCFAIGH LVGLASPESY
KEEWKKWSLQ TLPLIPEHFK YQVSKGKEKA FQVIRKLLQD SRIQEIVIAT DAGREGEAIA
WYIMKMSGVK NINDSRVPIK RLWISSLTEA GVKKGFSSLR NASETYSYYE EANARGIADW
TVGMSMSRAT TILLHHHDQT LRDSGIFSVG RVQTALLALL KKREDEILNF KSEPFWNVFA
IFDVNGKQYE GKWFNEQSDR FMGQQEALKV VSSCKGKTST IKEVKKERKA SKAPMLFNLS
ALQTKANKVY KMSPQKVLEI AQSLYTKGFL SYPRSDSQYL TENEARELPA ILQKLRNKAE
YQNLIPTPKS SIEQDKRYTN ASKVSDHHAI IVTEQIPSNL ADDEQKIYDL VARSVIAAFY
EDAIFDHTHI VTQVESFSFV TRGKQLIQEG WRKILFPNGE KSEEGENQEE DTILPPLSEH
EVGVIKDIKT KEGKTQPPKR YSQGDLITLM KTAGKAAEIS EEDWDEDGLS LKEAEKLGSI
GTEATRAGII QTILDRQYIK ISKNKVFVTD KGNILIESLI KSQCELVSPI MTAKWEHKLG
QIGEKKYPAF RFTEEIKALT TGMVKQIIQS APLWNFRTQI EHMQKEELIG LCPKCQSPIK
EFQTKDKKRF FACTGYKDGC KFAFNKSFLN KSLTPNQVKK LLDKGKTDVI KGFKTSGGKT
FDTFLWLTDE YKIDFGFNHP EEKKKREENK PKQIGICPKC SKALMEHPTF IGCSGYKDGC
KYAIPKVFLK KKLPISAIKS LLKGEPTEII EGFEGKSGKP FNAKLNYNLD EMKIDFIFDN
QKVEK
//
