ID A0A2N3XSV3_SACSN Unreviewed; 631 AA.
AC A0A2N3XSV3;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 24-JAN-2024, entry version 15.
DE RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
GN ORFNames=A8926_1325 {ECO:0000313|EMBL:PKW13768.1};
OS Saccharopolyspora spinosa.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharopolyspora.
OX NCBI_TaxID=60894 {ECO:0000313|EMBL:PKW13768.1, ECO:0000313|Proteomes:UP000233786};
RN [1] {ECO:0000313|EMBL:PKW13768.1, ECO:0000313|Proteomes:UP000233786}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44228 {ECO:0000313|EMBL:PKW13768.1,
RC ECO:0000313|Proteomes:UP000233786};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 Actinobacteria strains.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PKW13768.1}.
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DR EMBL; PJNB01000001; PKW13768.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2N3XSV3; -.
DR STRING; 994479.GCA_000194155_04043; -.
DR OrthoDB; 9813184at2; -.
DR Proteomes; UP000233786; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675}.
FT DOMAIN 46..362
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 422..533
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 550..608
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 194
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 270
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ SEQUENCE 631 AA; 69484 MW; 3C3714DE9553D268 CRC64;
MGVNRRGFPG SIALGGLALG SSMVVGTNRV VAQAQQPAGL TVRGDQFLLD GEPFRIVAGE
MHYFRTHPDH WRDRLARMRA LGLNTVDTYV AWNFHEPRRG AVDFSSWRDL VRFVETAAEV
GLKVAVRPGP YICAEWDFGG LPAWLLADPD LPLRCDETAY PDLVDEWFGV LLPRLAPLQA
TRGGPVIAFQ VENEYGSYAN DQAHLDHLRK TMRDNGIDSL LYCSNGPSEW MLRGGNLPDV
LATVNFGGDP TEPFAALRRY QPEGPLWCTE FWDGWFDHWG EPHHTTDPVE TAADVEKILA
AKASVSLYMA VGSTNFGWWA GANFDEANGT YQPTITSYDY DAPIGEAGEL TTKFHRIREV
IARHEPVPDE PLPPLPSRLP AQRVQPDATV GLLESLDALS ASSIEIEGST SMEIAPIHRS
APVHMEALGQ PYGLIHYRTH VQGPRETAGL WVRGLADRAL VFADGELLGT LDRNDPQGSV
EVPVQRESVQ LDLVVDTGGR INFGQRLNDP KGITDRVLHG SQALFGWEIR PLPLDDLSGL
GFRPGATSGP AFHRAVLRIG APADGFLALP GWEKGMVWLN GFLLGRYWSV GPQRTLYAPS
PLWRTGENEL IVLELHRRGG STELRSEPDL G
//
