ID A0A2N3XZ36_SACSN Unreviewed; 589 AA.
AC A0A2N3XZ36;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE SubName: Full=Hydrogenase large subunit {ECO:0000313|EMBL:PKW15889.1};
GN ORFNames=A8926_3669 {ECO:0000313|EMBL:PKW15889.1};
OS Saccharopolyspora spinosa.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharopolyspora.
OX NCBI_TaxID=60894 {ECO:0000313|EMBL:PKW15889.1, ECO:0000313|Proteomes:UP000233786};
RN [1] {ECO:0000313|EMBL:PKW15889.1, ECO:0000313|Proteomes:UP000233786}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44228 {ECO:0000313|EMBL:PKW15889.1,
RC ECO:0000313|Proteomes:UP000233786};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 Actinobacteria strains.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PKW15889.1}.
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DR EMBL; PJNB01000001; PKW15889.1; -; Genomic_DNA.
DR RefSeq; WP_010696688.1; NZ_PJNB01000001.1.
DR AlphaFoldDB; A0A2N3XZ36; -.
DR STRING; 994479.GCA_000194155_03550; -.
DR OrthoDB; 9761717at2; -.
DR Proteomes; UP000233786; Unassembled WGS sequence.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR42958:SF4; HYDROGENASE EXPRESSION_FORMATION PROTEIN HUPK; 1.
DR PANTHER; PTHR42958; HYDROGENASE-2 LARGE CHAIN; 1.
DR Pfam; PF00374; NiFeSe_Hases; 2.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|PIRSR:PIRSR601501-1}.
FT REGION 331..350
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 69
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 72
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 72
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 562
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 565
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 568
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 589 AA; 66581 MW; D87B9B98C6880064 CRC64;
MSAQQRNLVE VEFDPITRII GNLGIYTKVD FDNSEVVECR STSSLFRGYS VFMKGKDPRD
AHFITSRICG ICGDNHAVCS IYAQNMAYGV KTPPLGEWII NLGEAAEFMF DHTIFQDNMV
FVDYCEKMVS ETNPSLLQRA EQTDAPRGNV HGHRTIADIM RSFNPFEGET YKAALEMSRV
TREMCCLMEG RHVHPSTLYP GGVGTVATPQ LFTDYQVRLV RVLDFIKRAV AMNDDVFDFF
YEALPGYEEV GRRRTLLGCW GCFQDPDVVD YDYRNMSEWG RSMFVTPGIV VDNELLTTDL
VDINLGMRIL LGSSYYEDWA NEQTFVDRDP LGNPVDKRHP WNQTTLPQPQ KRELDGGKYS
WVMSPRWFDE RSGDHLALDT GGGPIARLWA TALAGRVNTP YVRSTGHSVQ IDLPKGQSLP
DMQLEWRPPQ FPNTLERNRA RMYFVAYAAG MAQYFLDRAL AEVRSGNTKV FEDFNVPDEA
IGVGFHEAVR GVLSHHLVIR DGKIANYHPY PPTPWNASPR DSYGTPGPYE DAVQGQPIFE
ENGRDEFKGI DIMRTVRSFD PCLPCGVHMY LGGGRVLERT HSPMFGAGS
//
