ID   A0A2N3Y6B8_SACSN        Unreviewed;      3789 AA.
AC   A0A2N3Y6B8;
DT   25-APR-2018, integrated into UniProtKB/TrEMBL.
DT   25-APR-2018, sequence version 1.
DT   24-JAN-2024, entry version 21.
DE   SubName: Full=Non-ribosomal peptide synthase protein (TIGR01720 family)/amino acid adenylation domain-containing protein {ECO:0000313|EMBL:PKW18476.1};
GN   ORFNames=A8926_6563 {ECO:0000313|EMBL:PKW18476.1};
OS   Saccharopolyspora spinosa.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Saccharopolyspora.
OX   NCBI_TaxID=60894 {ECO:0000313|EMBL:PKW18476.1, ECO:0000313|Proteomes:UP000233786};
RN   [1] {ECO:0000313|EMBL:PKW18476.1, ECO:0000313|Proteomes:UP000233786}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 44228 {ECO:0000313|EMBL:PKW18476.1,
RC   ECO:0000313|Proteomes:UP000233786};
RA   Klenk H.-P.;
RT   "Sequencing the genomes of 1000 Actinobacteria strains.";
RL   Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC         Evidence={ECO:0000256|ARBA:ARBA00001957};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PKW18476.1}.
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DR   EMBL; PJNB01000001; PKW18476.1; -; Genomic_DNA.
DR   STRING; 994479.GCA_000194155_03109; -.
DR   OrthoDB; 2378856at2; -.
DR   Proteomes; UP000233786; Unassembled WGS sequence.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:UniProt.
DR   GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR   GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR   GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR   GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR   CDD; cd05930; A_NRPS; 1.
DR   CDD; cd17643; A_NRPS_Cytc1-like; 1.
DR   CDD; cd19540; LCL_NRPS-like; 1.
DR   Gene3D; 3.30.300.30; -; 3.
DR   Gene3D; 3.40.50.980; -; 4.
DR   Gene3D; 1.10.1200.10; ACP-like; 2.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 4.
DR   Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR   Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 4.
DR   InterPro; IPR010071; AA_adenyl_domain.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR   InterPro; IPR042099; ANL_N_sf.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR001242; Condensatn.
DR   InterPro; IPR010060; NRPS_synth.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR006162; Ppantetheine_attach_site.
DR   InterPro; IPR001031; Thioesterase.
DR   NCBIfam; TIGR01733; AA-adenyl-dom; 3.
DR   NCBIfam; TIGR01720; NRPS-para261; 1.
DR   PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR   PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR   Pfam; PF00501; AMP-binding; 3.
DR   Pfam; PF13193; AMP-binding_C; 3.
DR   Pfam; PF00668; Condensation; 4.
DR   Pfam; PF00550; PP-binding; 3.
DR   Pfam; PF00975; Thioesterase; 1.
DR   SMART; SM00823; PKS_PP; 3.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 3.
DR   SUPFAM; SSF47336; ACP-like; 3.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR   SUPFAM; SSF52777; CoA-dependent acyltransferases; 8.
DR   PROSITE; PS00455; AMP_BINDING; 3.
DR   PROSITE; PS50075; CARRIER; 3.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE   4: Predicted;
KW   Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}.
FT   DOMAIN          964..1038
FT                   /note="Carrier"
FT                   /evidence="ECO:0000259|PROSITE:PS50075"
FT   DOMAIN          2432..2507
FT                   /note="Carrier"
FT                   /evidence="ECO:0000259|PROSITE:PS50075"
FT   DOMAIN          3459..3534
FT                   /note="Carrier"
FT                   /evidence="ECO:0000259|PROSITE:PS50075"
FT   REGION          436..456
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          941..965
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   3789 AA;  406194 MW;  25328CAFBA85DECB CRC64;
     MTSHDQATEL PVSEAQLGIW LAEQAGSASS SPYLWGEYAE ITGPLEVGLL CTAVRLAARE
     AEAVNTTFTE ASDGRPRQHL RQLEPAVAVH DLSGADDPHG AALSWMRAEL DRPLELRDGP
     VHGAVVLRLA AQRHLLFQWA HHIALDGVGM TMLTTRIAVI YDHLHRGLPV PAGQWGGLAG
     LLAEEDGYRS SAEFDADRRW WHEHLADGPG FLSMAARPAA AAASSLRLTT TFGEAKFAEL
     RAAADRFGRR WSRLVIAATA LHTQAMSGSR EVVLSLPVPG RFSGFSWTVP SMSANVLPLR
     LRLDPADTVA ELLGQVCDRV RSVLAHQRYR GERLRREAGY SEDGRRFFGP VVNVQKFAYG
     LSFGDGTATV HNVAAPPSED FSVVAYDRGD GLLRFDFDAN PANHDRAVLA AARDRFLHLL
     EQLVRADPGT RIARLETTTP AERAPVRSAG TGAAGSAPAQ DLVSRFAERV AAAPEAFAVR
     APATGERLTY RELDARAEAL AARLVAAGAG AESPVGLLVE RSVGLVVAVL AVVRAGAAYM
     PLHRADGAHR LRSIADGADL ALLITDEATA QDEFTGWFRN TGTVVDVAAG GGTAPVTAAA
     ITPGRLACVM FTSGSTGTPK GVAITHENVV RLTEDRWWAE GAAERVLLHS PHAFDALTLE
     LWVPLLTGGE VVLAPPGEAD LQVLGEVVAG CGVTGLWLTA GLFDAVSQEA PGWLRGVRQV
     WTGGDVVPAA AVRRVRAVVP ELVVVNGYGP TETTVFATRH VVDSEDATAV PIGAPIDGKR
     VHLLDAALRP VPLGAVGELY LAGFGLARGY LADPAATVQR FVACPYGAPG ERMYRTGDLA
     RWNPDGTLHF AGRGDGQVKL RGHRIETGEV DAALGRAEGV RQAATVLREG ETGRRLVSYV
     VAEQADPAAL RQHVAALLPP FMVPSQFVVV AELPLNRNGK LDRAALPDPA EDSAPQEAPT
     ESPVPASAAE ELLAGLFREV LGLAEVPPDR DFFALGGDSI KAIQLASRAR RGGLELGTSD
     VFRHPTVAAL AKRVGAAAPA LPGPGAAVGP LPLTPIMHWL RERGGALAGF AQCATLRVPA
     VGEQGLRSVL SSLVRRHDAL RLKLTAAGGL WNLQVAAQAG YDFARVDVRE LAEQRRRELA
     GQWARRAQAR LDIETGPAVS AVWLDAGGEP GTLVLAVHHL AVDGVSWRIL LDDLREAGQV
     VLGGRSPRAE VAGTSFAQWA RALPEHAREP GTQAEFPWWR QVAEVSGPSA QPGAESDRGH
     LSLQLPEEIT AGLLEQATAV FGCSADTLLL SAFALAVADG PAVLVDVERH GREEFGGFDL
     SRTVGWFTTQ FPVQLQPGAA WSQVQQDRRL LGEVVENVRA ALGAVPRNGF GYGLLRYLNP
     QSAPVLAGGP RPRFGFNYLG RFDAGSDADW CPRPLAPVLG SSMPQDMRMV HLVELDCAVT
     DEPAGPRLTA NWSWDGQLLG EREAAVLGER WFAVLGAMAE HAREGAGQRA DTVLPLPPLA
     QGLLFHSMYD AQAADPYLVR FVFELTGELD EQRLREAVHA LLRRHPQLSA AFRHDAEGVP
     VQVWPASFNI EWTRREDATA QEVSRFLDAD RARRFDLAEA PVWRAALLGA GADRHVLVLS
     THHIVIDGWS TPILINDLFA LYAGAALPAP ADYRRFLDWT RERDGAAHEE VWRTILSDVD
     GPTLVAPAAG RGAEDRGRVL DRLDAVGTDA VRRTAQRLGS TVSTVVQLAW AMLLGALTGR
     DDVVFGATVA GRPPELPGVE DVVGLLINTV PVRVRLDPRR TVAEALAALR DQQLAVLEHQ
     HADLTRLQAI AGYGELFDTA VVFENYPSDD PDAHPVPGLR VTGVEVLDGT HYPLSLIVVP
     GDQLGFRLDH RLDVLGEAAA RRLLRRLAAV LARIGATPHS RLGEITLLLP GEDLDTAAIS
     PPGLQHAALP ELFEVHAVAH PEAVAVSFGA ERLTYGELNE RANRLARLLI ARGAGPERLV
     ALALPRSLDL VVALLAALKS GAGYLPLDPE HPSERIRRTT ADAEPVVIVS VRALRPVLPA
     HPGALLLDDP RTRAELAAAG AEALRVPVHP DHAAYVIYTS GSTGEPKGVV VSHRNVLRLL
     EVTDGLFGFG PDDVHALFHS CAFDVSVWEL WSALGRGGRL VVVPGEVTRA PRALRDLLDA
     EGVTALSQTP SAFYQLAQEE PGGGLPALRH VVFAGEALDP ARLRGWHGQQ RPRLVNMYGI
     TETTVHATWA LLSDPAETRS VIGTALADLR LHLLDHALRP VPPGCPGEIY LGGPGVARGY
     LGRAGLTAAR FVADPFGPAG ARLYRSGDLA RALPDGSLEY LGRVDLQVKV RGFRIEPAEV
     EHVLELHPSV ARAVVLAQHD DTGGGRLVAY VVPTTEVDPA QLREHAAMLL PAHMVPAFVV
     AVAEIPLTAN GKLDRAALPE PGSGRGRAAG RAPGGPLERF LCEAFAATLG IPEIGVQESF
     FDSGGHSLLA TRLINRVRSG LGVELDIRTL FDAPTVAELA GHVARARTRD RPSLRPDRRP
     QHVPLSPAQR RLWFISGFDE FATTYNMRFA VRLDGPLSAT ALRAALGDVV ARHESLRTVV
     AESDDGPWQQ VRSPEQLRFE HVQVTEADLA ARLAADSAHR FDLAAELPIR ATLYEHGPGQ
     HVLLVLLHHI AVDGWSLAPL ARDLSTAYRA RSTGRSPHWP TSPVQYADFT VWQQAIDAEP
     QLRHWTKVLA GAPELLPLPT DHPRPPVSAH RGETIEVALD AKLHAAVLRL AQDHDVSPFM
     VLHAAFAALL CRLGAGEDIP IGTPVAGRND AALHDAVGCF VNTVVLRTDL SGRPSFSELL
     RRVRAADLDA FDHQEVPIER LVELLNPARS LGHHPLFQVM LAFQDTPSQE FDLPGISTEQ
     VALHGESSRL DLLLSLRQNH TPTGMPEGIT GVLEYDTELF APDTARKLIS RFELLLRAAA
     DAPGTPIAEL PVVSEQEHHW LVHGCNDTAR QAPPTPLHEL FAAQVARTPH AEAVLHDHAA
     LTFRELDQRV REIAAQLTEH GIGPGALVAS ALPRTPDLLA TMLAVMRTGA ACLPCEPDWP
     AARTDAVLDD ARPALVVGTD ARGELSFTRP GRSPAPIATG TAYVIYTSGS TGRPKGVVVA
     HRAVVNLLAA LAELLEVGAR DRVLATAPAG FDMSVPEFYL GALTGAAVVL ADRDTTRDPD
     ALLELVERQR VTVMHATPSL WRTLAAGRPR ALRGIRGVIG GEAVPGPLAE RLRGLGVRLL
     ACYGPTETTV WSSACPVEGR QREVVPLGRP LWNTACHVLD ERLRPAPPGA VGELYISGLG
     VATGYLHRAA LSAQRFVADP FGPPGTRMYR TGDLASRAPD GSLRFHGRAD EQVKIRGHRV
     EPSEVEAALL GHPQVRAAAV TVHAHDAHDR RLVAHLVAPG VDLASVREHL RARLPGYMIP
     SRLLTCDELP LSANGKLLRD RLPTPAEDTA AEQSAAGEPD VAAPVRVLCE IFAEVLGTAR
     VGPEENFFEL GGHSLITAPL IARIRSRLGL RAKVRDVFEA GTPAALHRAL TGPSAAGRHL
     LPLRSGGQAA PLYCVHPLSG LGWSYAGLLA HLDHDQPVYA LQGDAGARPP GSIEEMAERY
     LQEIRSAHPR GPLHLLGWSF GGLVAHHMAI KARERDLQVG LLCLIDPPLG AEPAPIDAQR
     VYRILLTAIG HDDQPEGDLA FEAVSQLLRR DDGAFAAFTE AGIARTVATA RHNAELLRGH
     RPGHYPGDAV HIAAEAGPGA RAWRAHVGGE LATYRIDLPH DRLMQPEHVG EIARIVRDHL
     TEQKGHGIP
//