ID A0A2N3YKN2_9MICO Unreviewed; 815 AA.
AC A0A2N3YKN2;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN ORFNames=ATL31_2200 {ECO:0000313|EMBL:PKW27359.1};
OS Phycicoccus duodecadis.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Phycicoccus.
OX NCBI_TaxID=173053 {ECO:0000313|EMBL:PKW27359.1, ECO:0000313|Proteomes:UP000233781};
RN [1] {ECO:0000313|EMBL:PKW27359.1, ECO:0000313|Proteomes:UP000233781}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 12806 {ECO:0000313|EMBL:PKW27359.1,
RC ECO:0000313|Proteomes:UP000233781};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 Actinobacteria strains.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618,
CC ECO:0000256|RuleBase:RU364053};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PKW27359.1}.
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DR EMBL; PJNE01000001; PKW27359.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2N3YKN2; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000233781; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000233781}.
FT DOMAIN 67..359
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 360..646
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 88..95
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 815 AA; 89725 MW; 2D8CE969E602AA19 CRC64;
MSTLFDGLDF GVNAATPPAD PATHASAQAA LVNAAGVPTW AEAAAAGRGP DGGPVGPRAL
DADALLDGLN PQQREAVLHA GPPLLIVAGA GSGKTRVLTH RIAHLLADRG VQPGQVLAIT
FTNKAAAEMR ERVASLVGPR AKAMWVMTFH SACVRILRRE ADKVGMRSTF SIYDAADSQR
LMAMVLRDMD LDPKRYNPRA FGHQVSNLKN ELVDEETFAS RVAQEGTHQE QVLAEAYTAY
QRRLRQANAM DFDDLIMTTV HMFQAFPEVA EHYRRRFRHV MVDEYQDTNH AQYQLVKELV
GAEATRAEHG VEPSELVVVG DADQSIYAFR GATIRNIEEF EEDYPQARTI LLEQNYRSTQ
TILRAANAVI ARNETRRKKN LWTDSGDGAP IIGYVADNEH DEASFVARTI DRLGDDHGVR
PGDVAVFYRT NAQSRALEEV FVRVGLPYKV VGGTRFYERR EVKDALAYLR VLSNPTDTVN
LRRILNVPKR GIGDRAEACV AALAERERIP FVAALGRPQD APGIATRSVA AITGFTTILE
DLGRVRDDED AGVADLLEAI LDRTGYVAEL RESRDPQDET RVENLAELVA VAREFDEARR
ETGEVVSLED FLERVSLVAD ADEIPDSAEA EEQGVVTLMT LHTAKGLEFP VVFLTGMEDG
TFPHLRSLGD PKELEEERRL AYVGITRARE RLHLSRAAVR SAWGAPQYNP PSRFLDEIPV
ELLSWERELS GAAAVGRRDQ RPAVATLAAR PGVRSPGNRP IIALSPGDRV THDSYGLGTV
VRTMGEGDRT QAEVDFGGEL GVKRFVLRYA PLEKL
//
