ID   A0A2N5GZV1_9BACI        Unreviewed;       763 AA.
AC   A0A2N5GZV1;
DT   25-APR-2018, integrated into UniProtKB/TrEMBL.
DT   25-APR-2018, sequence version 1.
DT   27-MAR-2024, entry version 15.
DE   SubName: Full=Family 65 glycosyl hydrolase {ECO:0000313|EMBL:PLR96542.1};
GN   ORFNames=CVD19_11145 {ECO:0000313|EMBL:PLR96542.1};
OS   Bacillus sp. T33-2.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=2054168 {ECO:0000313|EMBL:PLR96542.1, ECO:0000313|Proteomes:UP000235118};
RN   [1] {ECO:0000313|EMBL:PLR96542.1, ECO:0000313|Proteomes:UP000235118}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T33-2 {ECO:0000313|EMBL:PLR96542.1,
RC   ECO:0000313|Proteomes:UP000235118};
RA   Seuylemezian A., Cooper K., Vaishampayan P.;
RT   "Comparitive Functional Genomics of Dry Heat Resistant strains isolated
RT   from the Viking Spacecraft.";
RL   Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 65 family.
CC       {ECO:0000256|ARBA:ARBA00006768}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PLR96542.1}.
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DR   EMBL; PGVB01000013; PLR96542.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2N5GZV1; -.
DR   OrthoDB; 9758855at2; -.
DR   Proteomes; UP000235118; Unassembled WGS sequence.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 2.70.98.40; Glycoside hydrolase, family 65, N-terminal domain; 1.
DR   Gene3D; 2.60.420.10; Maltose phosphorylase, domain 3; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR005194; Glyco_hydro_65_C.
DR   InterPro; IPR005195; Glyco_hydro_65_M.
DR   InterPro; IPR005196; Glyco_hydro_65_N.
DR   InterPro; IPR037018; Glyco_hydro_65_N_sf.
DR   InterPro; IPR017045; Malt_Pase/Glycosyl_Hdrlase.
DR   PANTHER; PTHR11051; GLYCOSYL HYDROLASE-RELATED; 1.
DR   PANTHER; PTHR11051:SF13; GLYCOSYL TRANSFERASE-RELATED; 1.
DR   Pfam; PF03633; Glyco_hydro_65C; 1.
DR   Pfam; PF03632; Glyco_hydro_65m; 1.
DR   Pfam; PF03636; Glyco_hydro_65N; 1.
DR   PIRSF; PIRSF036289; Glycosyl_hydrolase_malt_phosph; 1.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000313|EMBL:PLR96542.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000235118}.
FT   DOMAIN          9..241
FT                   /note="Glycoside hydrolase family 65 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03636"
FT   DOMAIN          318..679
FT                   /note="Glycoside hydrolase family 65 central catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF03632"
FT   DOMAIN          688..747
FT                   /note="Glycoside hydrolase family 65 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03633"
FT   ACT_SITE        479
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-50"
FT   BINDING         352..353
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
FT   BINDING         591..592
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
SQ   SEQUENCE   763 AA;  86470 MW;  EAF33EF5E70A82E4 CRC64;
     MTWILTDNQL TQQGLLLAES LFSLGNGYIG VRGNFEEGYA EGMSSIRGTY QNAFHDIIDI
     PYGEKLFAFP ETQQKIVNVI DAQTVEIFLG DDEERFSLFE GEILSYSRQL NLADGYLTRT
     AHWRSPNGKE AIITFRRVVS FVVRELFAID ILLEPVNFHG PVKVVSAVKG AVSNFVDSKD
     PRVGSGHAKR LHVKDTGVRD NLIFVVDETY TSKLQTACVI SHQIPEGSRA EFNKTDTGAK
     AVFSFALTHA ARITKWNIYT DTLRHGEEVL QRGLEIQQQI SNSSFEEILE KQKEYLDAYW
     DGADITIGGD EKLQEGIRFN LFHLLQSVGK DPFSNIAAKG LSGEGYEGHY FWDTEIYIFP
     LFLMVNPKLA RQLLFYRYSI LDHARERAKE MGHKKGALFP WRTISGKECS SYFPAGTAQY
     HISADVAYSY VQYYLATADD NFLMEFGAEV LFETARLWMD AGHYHNGSFK IDAVTGPDEY
     TCIVNNNYYT NVMAKYNLAW AAKVYIRLEE LGAAELADLI SRIHVSADEA SEWKQAAGSM
     YLPYDETLKI NPQDDSFLQK AVWDFESTPD NHYPLLLHYH PLTLYRYQVC KQADTVLAHF
     LLEDEQELET IRNSFHYYEK ITTHDSSLSS CIFSIMASKI GAHGKAYEYF IETARLDLDN
     THGNTKDGLH MANMGGTWMS IVYGFAGLRL KESGISLAPA IPDSWSGYAF KLQYQGRKLE
     IGINREETSV CLLSGEPMAI KLYGQKIMLE KGHPYRAKLL PQN
//