ID A0A2N5WKZ2_9ACTN Unreviewed; 593 AA.
AC A0A2N5WKZ2;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 12.
DE SubName: Full=Hydrogenase {ECO:0000313|EMBL:PLW62907.1};
GN ORFNames=C0036_28005 {ECO:0000313|EMBL:PLW62907.1};
OS Streptomyces sp. DJ.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2067551 {ECO:0000313|EMBL:PLW62907.1, ECO:0000313|Proteomes:UP000235150};
RN [1] {ECO:0000313|EMBL:PLW62907.1, ECO:0000313|Proteomes:UP000235150}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DJ {ECO:0000313|EMBL:PLW62907.1,
RC ECO:0000313|Proteomes:UP000235150};
RA Ke Y., Yu G., Ye J., Yuan X.;
RT "Genome of Streptomyces sp. DJ strain.";
RL Submitted (JAN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PLW62907.1}.
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DR EMBL; PKSK01002046; PLW62907.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2N5WKZ2; -.
DR Proteomes; UP000235150; Unassembled WGS sequence.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR42958:SF4; HYDROGENASE EXPRESSION_FORMATION PROTEIN HUPK; 1.
DR PANTHER; PTHR42958; HYDROGENASE-2 LARGE CHAIN; 1.
DR Pfam; PF00374; NiFeSe_Hases; 2.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|PIRSR:PIRSR601501-1};
KW Reference proteome {ECO:0000313|Proteomes:UP000235150}.
FT BINDING 75
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 78
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 78
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 565
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 568
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 571
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 593 AA; 66983 MW; 20DA05ECAAA675EC CRC64;
MAAPTQAAGD GSGLVEMAWD PITRIVGSLG IHTKIDFKQK RVAECYSTSS VFRGYSVFMR
GKDPRDAHFI TSRICGICGD NHATCSVYAQ NMAYGVRPPH LAEWIINLGE AAEYMFDHNI
FQENLVGVDY CEKMVRETNP GVLELAERTE APHAGDHGYR TIADIMRSLN PLEGEFYREA
LQVSRYTREM FCLMEGRHVH PSTLYPGGVG TIASVQLFTD YLSRLTRYVE FMKRVVPLHD
DLFDFFYEAL PGYEEVGRRR VMLACWGALN DPEHCDFTYR NMTDWGRRMF VTPGIVVDGE
LVTSDLTEIN LGIRILLGSS YYEDWEGREL FVERDPLGNP VDPRHPWNQH TVPAPQKRDF
DGKYSWVMSP RWFDGKEHLA LDTGGGPLAR LWSTALSGLV DTGYVKATGH SVVINLPRTM
TRPETTFEWR IPRWSNALER NRARTYFQAY TAALALHFAE KGLEEVRAGR TQTWERFEVP
DEAVGVGFTE AVRGVLSHHM VIRDGKIANY HPYPPTPWNA SVRDSYGTPG PYEDAVQNTP
IFEENPPEDF KGIDIMRAVR SFDPCLPCGV HMYVGGRTVQ TMHVPTGLSG LAG
//
