ID A0A2N6P2F8_BEABA Unreviewed; 1187 AA.
AC A0A2N6P2F8;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN Name=prp22 {ECO:0000313|EMBL:PMB73696.1};
GN ORFNames=BM221_001120 {ECO:0000313|EMBL:PMB73696.1};
OS Beauveria bassiana (White muscardine disease fungus) (Tritirachium
OS shiotae).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Cordycipitaceae; Beauveria.
OX NCBI_TaxID=176275 {ECO:0000313|EMBL:PMB73696.1, ECO:0000313|Proteomes:UP000235728};
RN [1] {ECO:0000313|EMBL:PMB73696.1, ECO:0000313|Proteomes:UP000235728}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEF-007 {ECO:0000313|EMBL:PMB73696.1,
RC ECO:0000313|Proteomes:UP000235728};
RX PubMed=27470140; DOI=10.1007/s00253-016-7734-y;
RA Kim S., Lee S.J., Nai Y.S., Yu J.S., Lee M.R., Yang Y.T., Kim J.S.;
RT "Characterization of T-DNA insertion mutants with decreased virulence in
RT the entomopathogenic fungus Beauveria bassiana JEF-007.";
RL Appl. Microbiol. Biotechnol. 100:8889-8900(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PMB73696.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MRVG01000001; PMB73696.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2N6P2F8; -.
DR OMA; DPMVAPE; -.
DR Proteomes; UP000235728; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:1990904; C:ribonucleoprotein complex; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd21691; GH2-like_DHX8; 1.
DR CDD; cd05684; S1_DHX8_helicase; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR049588; DHX8_GH2-like.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049621; S1_DHX8_helicase.
DR InterPro; IPR003029; S1_domain.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF85; ATP-DEPENDENT RNA HELICASE DHX8; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50126; S1; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000313|EMBL:PMB73696.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000235728}.
FT DOMAIN 224..295
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
FT DOMAIN 533..696
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 714..894
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 78..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 134..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 315..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 435..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 81..100
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..160
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 171..220
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..463
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1187 AA; 133559 MW; B63B5F71BB8CE388 CRC64;
MDDFLNLELL SLVSKVTSEL QNHVGVSDKT LAEFLIAQRI ESKNADEFRS KLDGLGADFP
PSLVDSLDRL VLTMHPRFKG SKGAAEPKEE HHGRSLEEKE KAFSGLALAD GKPSYRDDDG
DAIDDTLALL EGLEGKARKD KGTRKRSRSP HDESRRRRRD RSRSREGRKR DRFRSRSPSQ
DRGGQDSRNG YRDSRKSRHG RRDDRYDDLR HAPAPEMDDE PQLNKVYEGH VTGIKDFGAF
VNLHNVRGRQ DGLVHISKIA AHQRVNHPSD LLEKGKNVWV KVVSVEGKRI SLSMASVDQS
TGMDKEPQVS FTSGANMEAL GGGGRGFNDV PSGMPRDDIG APRKHKKRMT SPERWEIRQL
IASGVAKASD YPDLEEEYNA TLRGDGELEL EEDVDIEVRE EEPPFLAGQT KQSLELSPIR
VVKAPDGSMN RAAMSGTALA KERKELKQQE AEAAKERPKE NLSSQWEDPM ADPQKRQFAS
DLRNAKTNAG TEDVPEWKRA IVPRNQTLGK RTNLSMKEQR ETLPVYAFRS QLIKAVQENQ
ILIVVGETGS GKTTQLTQYL AEGGFANDGV IGCTQPRRVA AMSVAKRVAE EVGCKLGEEV
GYYVRFDDMT GPMTKIKYMT DGMLLREVLG DPDMKRYSVI MLDEAHERTI STDVLFALLK
KALKRRPDLK VIVTSATLDA DKFSMYFNEC PIFTIPGRTF PVEILYSREP ESDYLDTALV
TVMQIHLTEP KGDILLFLTG QEEIDTACEV LFERMKALGP GVPELLILPV YAQLPTEMQS
RIFDPAPPGA RKVVIATNIA ETSITIDEIY YVIDPGFVKQ SAYDPKLGMD SLIVTPISQA
QANQRAGRAG RTGPGKCFRL YTEAAYQSEM LPTTIPEIQR ANLAHVILML KAMGINDLLH
FDFMDPPPVN TMLTALEELY ALSALDDEGL LTRLGRKMSD FPMEPSLAKV LITAVDYQCS
EEMLSIVAML NQSTIFYRPK EKQTQADQKK AKFHDPHGDH LTLLNVYNSW KHSSYSRPWC
FENFIQDRSM RRAKDVRDQI LRIMERHRHP VVSCGRDTQK VRRALCTGFF RSAARKDPQE
GYKTLTEGTP VYLHPSSALF GKQAEWVIYH ELVLTTKEYM HWTTAIEPKW LVEAAPTFFK
VAPTDRLSKR KAQERILPLY NKFAGEDDWR LSAQKRGGRG GGGGTWG
//
