ID A0A2N7VKE2_9BURK Unreviewed; 665 AA.
AC A0A2N7VKE2;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=C0Z18_19495 {ECO:0000313|EMBL:PMS17644.1};
OS Trinickia dabaoshanensis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Trinickia.
OX NCBI_TaxID=564714 {ECO:0000313|EMBL:PMS17644.1, ECO:0000313|Proteomes:UP000235616};
RN [1] {ECO:0000313|EMBL:PMS17644.1, ECO:0000313|Proteomes:UP000235616}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GIMN1.004 {ECO:0000313|EMBL:PMS17644.1,
RC ECO:0000313|Proteomes:UP000235616};
RA Estrada-de los Santos P., Palmer M., Chavez-Ramirez B., Beukes C.,
RA Steenkamp E.T., Hirsch A.M., Manyaka P., Maluk M., Lafos M., Crook M.,
RA Gross E., Simon M.F., Bueno dos Reis Junior F., Poole P.S., Venter S.N.,
RA James E.K.;
RT "Whole genome analyses suggest that Burkholderia sensu lato contains two
RT further novel genera in the rhizoxinica-symbiotica group Mycetohabitans
RT gen. nov., and Trinickia gen. nov.: implications for the evolution of
RT diazotrophy and nodulation in the Burkholderiaceae.";
RL Submitted (JAN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PMS17644.1}.
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DR EMBL; PNYA01000018; PMS17644.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2N7VKE2; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000235616; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF2; BETA-GALACTOSIDASE YESZ; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Reference proteome {ECO:0000313|Proteomes:UP000235616}.
FT DOMAIN 6..389
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 405..607
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 142
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 313
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 103
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 141
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 321
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 665 AA; 75369 MW; 13C703A748DFD266 CRC64;
MRLGVCYYPE HWPEAMWNDD AARMRALGIE QVRIAEFAWS RIEPSPGEFD WGWLDRAIDT
LAAEELQVVM CTPTATPPKW LIDRHPDILP VGADGRVRAF GSRRHYDFSS PAYFEASKAI
CEAIAARYGK HPALAYWQTD NEYGCHQTVV SYSPAALERF RAWLKMRYGD IDSLNRAWGT
VFWSMEYRSF EEIDAPIGTV TEAHPSHRLD YRRFASDEVA RYNRMQVEII RKHSPGRPIA
HNFMQLFTEF DHYKVADDLD VAAWDSYPLG ALEEQWYEPE VKAKYLRTGH PDFASFNHDL
YRGMSKLPFW VMEQQPGPVN WAKWNPAPLP GMVRLWSWEA FAHGAGCVSY FRWRQAPFAQ
EQMHAGLNTP DNRLDIGGSE ARRVAQELLA LSDHTDADVQ GNVKARVALV FDYEAKWLFE
VHPQGIDFHY PRFAVEYYSA LRALGFDVDI VSTHASLDGY ALIVVPPLPV VPDDLARRLE
NTLAHVIIGP RTGSKTADLQ IPSNLPPGPL ASIMPVRVWR VDSMRPNVNP GVAFSGSVAP
GYARHWRDLI DVDLGKDSRE SIEIRAAFND GHPAYVKYGA VHYLASLFDE ASTQALFARI
AREAGLEPEP LGDTVRVSRR GGVTWVFNYG TQTHTMPSDI PSDDFLIGSR DIEPQGVAAY
RTRAR
//
