ID A0A2N8N5Z3_9BACT Unreviewed; 353 AA.
AC A0A2N8N5Z3;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN ORFNames=BHU16_08390 {ECO:0000313|EMBL:PNE24192.1};
OS Tannerella sp. oral taxon 808.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae;
OC Tannerella.
OX NCBI_TaxID=712711 {ECO:0000313|EMBL:PNE24192.1, ECO:0000313|Proteomes:UP000235918};
RN [1] {ECO:0000313|EMBL:PNE24192.1, ECO:0000313|Proteomes:UP000235918}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Cell103 {ECO:0000313|EMBL:PNE24192.1};
RA Beall C.J., Campbell A.G., Griffen A.L., Podar M., Leys E.J.;
RT "Genomics of the uncultivated, periodontitis-associated bacterium
RT Tannerella sp. BU045 (oral taxon 808).";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC {ECO:0000256|RuleBase:RU362028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PNE24192.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MIQB01000089; PNE24192.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2N8N5Z3; -.
DR Proteomes; UP000235918; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF44; PSEUDOU_SYNTH_2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028};
KW Reference proteome {ECO:0000313|Proteomes:UP000235918};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 38..103
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT ACT_SITE 163
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 353 AA; 40005 MW; 20072008762D7BCD CRC64;
MTLDQALADG LFSEEETDNE EALYEHFRFT ADPNQSLLRI DKFLTDRMTH TSRNRIQLAA
EAGWIRVDGQ PVKSNYRVKP GDIVTIVMTR PRHEFEILPE DIPLDIIYED PDLLVVNKPA
GLVVHPGFGN YTGTLVNALA FHLRNLPGYD PNDPRLGLVH RIDKDTSGLL VVAKNVDAKT
RLSRQFFEKT TSRRYLALSW GIPKEDEGRI EGNIGRDIHD RLRMAVYPAG SEEGKPAITH
YHVAERLGYV SLLECELETG RTHQIRAHMS HIGHPIFNDT RYGGDHILKG RPTGSYRQFV
LNAFALCPRQ ALHAETLGFI HPTTGRELFF SAPLPSDMQA LVDKWRDYCL SNT
//
