ID A0A2P1DMB9_9VIRU Unreviewed; 2917 AA.
AC A0A2P1DMB9;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Replicase large subunit {ECO:0000256|ARBA:ARBA00013540};
OS Nephila clavipes virus 4.
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Virgaviridae.
OX NCBI_TaxID=2108201 {ECO:0000313|EMBL:AVK59480.1};
RN [1] {ECO:0000313|EMBL:AVK59480.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC {ECO:0000313|EMBL:AVK59480.1};
RX PubMed=29118750; DOI=10.3389/fmicb.2017.02097;
RA Debat H.J.;
RT "An RNA Virome Associated to the Golden Orb-Weaver Spider Nephila
RT clavipes.";
RL Front. Microbiol. 8:2097-2097(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
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DR EMBL; MF348198; AVK59480.1; -; Genomic_RNA.
DR Proteomes; UP000289508; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd23254; Kitaviridae_RdRp; 1.
DR Gene3D; 2.60.120.590; Alpha-ketoglutarate-dependent dioxygenase AlkB-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001788; RNA-dep_RNA_pol_alsuvir.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022806};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000289508};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}.
FT DOMAIN 69..283
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000259|PROSITE:PS51743"
FT DOMAIN 1179..1269
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
FT DOMAIN 2022..2346
FT /note="(+)RNA virus helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51657"
FT DOMAIN 2658..2778
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
SQ SEQUENCE 2917 AA; 334978 MW; 64B8C2D301D9A680 CRC64;
MDLSKDTVVP PSELHTVCDT DFLQKKFLKE KLDSVLREER KKDTVTLKEI LKPAEQQLLS
SSYPEFHIVY ASEINHQHAF AAASRKLELH LLLLRLRVVP GYTLKNYDDY VCDIGGEFVV
HLLKGNGVHS CNPLLDIRDH QRLTTRIENL GRMVRNPKAF TFDLEKKNRI LEFLDGVPSV
SQKYYCNNKA QDCNRTARYG ICLHSAYDIS PFDFADIMLK KNMVEIYGSF IYSDLIFLHD
DGTIEGINCY FSYTKDRKFI EFSFHNDPSL IYRHSTVNYL ALLTLNSFKD KSGRYTFVIE
LLENRAGIQF FKITRINKEY EYSGLSKFLW LPSLKGKTKV KFFDLDQSAV ARGDFKKALE
PIEFYVDTAI IDKVVSHAYA STETKFRPVE IFNFLLAYNG RIFFGNDVCV RYNQLPHSKA
MALANAIYLY VYAKKYDIGK IDQLLIAAIK HDRELAEKSI LGKLWKGKFD YPSAYGSGHT
RFRFLRAMSS SFHRFLYWCN RSRYDAKSFL SENMQFIRCD DKETYSQIFF KIFNKKNKKI
SSVIDYKFSS VIPAGRSFEL KTKIAEIFSQ FCVHSTDAIV CLNDLVPVVD RIKISPRNEL
PSSIVAKIVK NNNCAGFSVL AGGSLEGDVL VVGNSKTFSE PLEIIGNAYV MGNCNLSTNS
INIAYDDLCL DCLSAISTLP INKVLLLPDN DLLIPVFNHC LRENITLYTW YSSEYTSENR
DLLALYLPYY NKKFFPMKYF RREPLEFDVL AGQLRELIDD DRDFTKFVLK NGTVTPDLRK
EVWIKMAKEQ KFFFIDKEDL IRNPDRDTIL KDLMRTKDVE DLDETLLFVC TVLSNFDFLF
YYQGFHEICN YVLNLVGFND GIAVMCYLVK KFFWIFMYGN GDNYLVLGDY FNSVVLRRNC
ALETCAVVTP ETFGLILCHL STWFLHRSTD FLDSIFDFFF VADPAMCFYY LACLYVDATA
ECVTIAEHYD ALASLSLRDI REECIYFYNS DINSKLNEFL RFPQQNLVED IPVINLELDV
GIILENSVLS SSTSGFSSCL STLDNFTNKN LDCSGSTSTL SSCLSTVGNS TDISDYVDCN
DLISEFTEDN ELLESESHEP VFLFENRVVI RTIPSDFVIF LKNFSDNLSS FKKTVRPGAK
GKRFEYLYSP MPYTYNNVTT YPINKNIPLD FFPYVDLSFY NTLLIVVYPS GTSGLNFHKD
DEPCMDSDRV LTFSVGADCT LKIKTLPNKK IFKYVLTSQY SYEFSHQRDF LHGVDTTGYR
VAFTFRKRRF TEVILNPLPI DVSTLEKFDN FGLDFYTNCE YSSDVLNNFN FVGSYSIDSK
ENLLSEIFDS EIWLKDLKKY CLLNNNRGWE DFLRNFGTLI EVSNCGSARL LICDIPSTDD
VVVMNTVIVE NILKSLWVLE SGGYLLLGVH LNSKLIKKLD LLGSFRVQKM FRPFSISPLS
RIAYLLVGGG SGSVNNISAL FNSANFHRAT YHTYLQHIKQ GATFNKPVLG NATVVECGGG
GDCLFYSLTA SDFSTACALR SLLLANKPLD INHPEYSVAD VDEECAAPGI HAGTSTIIAF
CEIFYSRVRI FSTVSKDLLF DYNPVKKPLY SIDLYYANNH YQRKSIKTIN EKRVQNFFLD
PVNLDSVYEK FLKPTFGEIP LCNHLVDLCL ENVCECLEGL NSFLSLGYDD FVFFSHTNYI
KDISNIFVNI TNNGYKVRFL CVPGLTNVGY IFMLISKYYS RGREVNLDFC YQLYVSHSKR
CSNCNNSNQI IIPPLYYHID RIYYFCNQAE LVSFTDSMED ADIVFKLEVN GLDPRDCYEL
IDNGDYVTVP VVLLSVNKLS DDISVLNAIR FLIDRFFSIR KSVPSMGLVN CGLDNLSFFS
DVFSKYTSKI YILKEEVSVN STGIVFNPTF LVNVDDNTYI KNSMIEAKEY YKSTISAIIS
KLQPTFKEYM DLFPYLKGGQ SFNKRKDFGI LDMGTGQFVI VPQTGGGPYS YAFDGVKLVD
ITTAIHQNFS LTDNLGRNTN WLKPGVFSGY MAVNSDTKLI NSEKIFKQMN KFDLNKITMG
FSIELIEGVP GCGKTRYIID NHEFSLTNCN HVVLTSSKEA VEDIRSRVAL KYKIDRDSFV
LQKRYRTVDS FLMHFDGRTR IETLWVDEGL MRHFGDLMWC ATISGCKTIK IMGDRAQVPF
HNRLAGVTLQ YHHLKISKSL KTTFLNTTHR CPVDVAVYLN RFGVYNRKVV STSRIDKSVS
AVLLSNISQL YAVANSTFLT FTKNERDTLR REGFSNVYTI NQFQGSQAEH VVLVRDNIKP
LPLFSSKNHQ LVAISRHTKS FVYFTRTLED DLYKFLLEPV TQSDIIGCSV PAKMLGGGVI
LGKTVTKQFP IFTIVPRAAT MIDLIKQDAR IRSFVQGNMG YGVVPMRINL LPIEYRANDL
PFAPSYYISD AITTMQYFYD EIFPGHSVQS YEYDNKIFQQ DDLFFATKEM MRMSSYERFF
SSKFDSLSPS LRTSCPSAVT NDLKNVLKAF YDRNGNVPEL QGLVNEEKMA DCMFNNFVNC
YIDNTDIFNN FSSEPIHVNV DSIEDWLFTQ DRKVKDQAFP EEFIDIFTRE LNVYSMILKK
LPKPKLENGA EYKFPSPQTI AHSSKDINAV FCPIVREMKR RLLAVMCPNV VMFTDISVSD
FEELLTVRLP AGVLRKMNFL LEADMSKYDK SQNLLALLYE IKMLRALGFP EFLIPTWIFM
HVYSKLVNRM LGLVFKIFYQ RKSGDPMTYF GNTLFLMAVV ASINIPRFSS LIKSYNVFLL
FSGDDFLAFC SVLIDLIHFS DEVAVRFNLE AKVLKYKTPY FCSKFLFMTP WGRWVVLPDV
VKMLTKLGRR DLVNFQHVEE YRISCADVGR NLGNSLLYPF IDDCMRDRYK LNLDSMVSLY
SAMFKCFSDS EEFGKLYYHD ERHNIDDQRV FTKLNEF
//
