ID A0A2P2DVQ3_9LEPT Unreviewed; 638 AA.
AC A0A2P2DVQ3;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:GBF48687.1};
GN ORFNames=LPTSP4_01870 {ECO:0000313|EMBL:GBF48687.1};
OS Leptospira ryugenii.
OC Bacteria; Spirochaetota; Spirochaetia; Leptospirales; Leptospiraceae;
OC Leptospira.
OX NCBI_TaxID=1917863 {ECO:0000313|EMBL:GBF48687.1, ECO:0000313|Proteomes:UP000245133};
RN [1] {ECO:0000313|EMBL:GBF48687.1, ECO:0000313|Proteomes:UP000245133}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YH101 {ECO:0000313|EMBL:GBF48687.1,
RC ECO:0000313|Proteomes:UP000245133};
RA Nakao R., Masuzawa T.;
RT "Novel Leptospira species isolated from soil and water in Japan.";
RL Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBF48687.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BFBB01000002; GBF48687.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2P2DVQ3; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000245133; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000245133}.
FT DOMAIN 5..147
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 188..424
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 638 AA; 72510 MW; D400C6BBE6541A85 CRC64;
MANLQLLQLP VPPPSYYAAT GNVPLAAASL ASSLEVSGLS KEIQAETILP EFTDRMGDQE
LVDYILSKDP DFLGLSLYLW NTERSLHLAK ELKKRNADLK ILIGGPEVNE DNPFVLSHTS
FDLAVRGESE HHFGYVMEAL LGKREFSSLS DVAYRTLDGG LSPFLTSRAA DFPLAQFPSP
YLTGHLAVEG NRSTYLETVR GCKSQCTYCF YPKSSQNLRT LDIDETKKLI IGLKDKGARE
LVFLDPTFNH RPGFEAFLDS IAEVNSDGQM NFFAELRSEG VSPKLAKKLA KAGFNRIELG
MQSINPETLK RVKRYGDPNK VAEVAKMLVG EGIELLLDLI VGLPGDKPED VERGLHFFVS
HGLEEWVQVF PLSVLPGTAM RRDAQKEGIT FIPYPPYRVV KTHTFSEEDI ILTMERAEEI
LDRRVDEYPR PYLCLSEETK EPTYVPLDEM KPSEAMVLAR RPGERHQAVW FATKHWERHI
ETILQFLSTR IELDPYCTLD VILYTEVELA SHYFQRIKQV LDGMSPNFLT RALAHRGENL
QRRLLCIAKP GLRMQKPWIN AMLDHSDVRL FLEADFHSMP KDLSQIGINL PSLWLTDRQL
GTEQWQYLKT NIDPEGICFK ETVWEKRWSL EVLGYGEV
//
