GenomeNet

Database: UniProt
Entry: A0A2P2GWE9_9ACTN
LinkDB: A0A2P2GWE9_9ACTN
Original site: A0A2P2GWE9_9ACTN 
ID   A0A2P2GWE9_9ACTN        Unreviewed;       389 AA.
AC   A0A2P2GWE9;
DT   23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT   23-MAY-2018, sequence version 1.
DT   24-JAN-2024, entry version 25.
DE   RecName: Full=Spermidine/putrescine import ATP-binding protein PotA {ECO:0000256|RuleBase:RU364083};
DE            EC=7.6.2.11 {ECO:0000256|RuleBase:RU364083};
GN   Name=potA {ECO:0000256|RuleBase:RU364083};
GN   ORFNames=VO63_01015 {ECO:0000313|EMBL:KKZ75822.1};
OS   Streptomyces showdoensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=68268 {ECO:0000313|EMBL:KKZ75822.1, ECO:0000313|Proteomes:UP000265325};
RN   [1] {ECO:0000313|EMBL:KKZ75822.1, ECO:0000313|Proteomes:UP000265325}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15227 {ECO:0000313|EMBL:KKZ75822.1,
RC   ECO:0000313|Proteomes:UP000265325};
RA   Thapa K.K., Metsa-Ketela M.;
RT   "Draft Genome assembly of Streptomyces showdoensis.";
RL   Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Part of the ABC transporter complex PotABCD involved in
CC       spermidine/putrescine import. Responsible for energy coupling to the
CC       transport system. {ECO:0000256|RuleBase:RU364083}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + polyamine-[polyamine-binding protein]Side 1 = ADP
CC         + phosphate + polyamineSide 2 + [polyamine-binding protein]Side 1.;
CC         EC=7.6.2.11; Evidence={ECO:0000256|RuleBase:RU364083};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotA),
CC       two transmembrane proteins (PotB and PotC) and a solute-binding protein
CC       (PotD). {ECO:0000256|RuleBase:RU364083}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Spermidine/putrescine importer (TC 3.A.1.11.1) family.
CC       {ECO:0000256|RuleBase:RU364083}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KKZ75822.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; LAQS01000001; KKZ75822.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2P2GWE9; -.
DR   OrthoDB; 9802264at2; -.
DR   Proteomes; UP000265325; Unassembled WGS sequence.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0015594; F:ABC-type putrescine transporter activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   CDD; cd03300; ABC_PotA_N; 1.
DR   Gene3D; 2.40.50.100; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005893; PotA-like.
DR   InterPro; IPR017879; PotA_ATP-bd.
DR   InterPro; IPR013611; Transp-assoc_OB_typ2.
DR   NCBIfam; TIGR01187; potA; 1.
DR   PANTHER; PTHR42781; SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA; 1.
DR   PANTHER; PTHR42781:SF4; SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA; 1.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF08402; TOBE_2; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF50331; MOP-like; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU364083};
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475,
KW   ECO:0000256|RuleBase:RU364083};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU364083};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW   ECO:0000256|RuleBase:RU364083};
KW   Reference proteome {ECO:0000313|Proteomes:UP000265325};
KW   Translocase {ECO:0000256|ARBA:ARBA00022967, ECO:0000256|RuleBase:RU364083};
KW   Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU364083}.
FT   DOMAIN          17..245
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000259|PROSITE:PS50893"
SQ   SEQUENCE   389 AA;  41751 MW;  8B0618776D75CCE1 CRC64;
     MTDKTAHTAK NAGGGDVRLS GISKSYGSFH AVRPLDLTIP QGSFFALLGA SGCGKTTTLR
     MIAGLEDPTT GTVFLGDRDV TDLPPYKRPV NTVFQSYALF PHMNIFENIA FGLRRRGIKS
     VKPQVDEMID LVQLGEHAHK KPHQLSGGQQ QRVAVARALI NHPQVLLLDE PLGALDLKLR
     RQMQLELKRI QTEVGITFVH VTHDQEEAMT MADQVAVMNG GRVEQLGAPA DLYENPQTTF
     VANFLGTSNL IEAEVVETGA DVLATAGGGR LRVPGARCTD AVRTGGKVLV GVRPEKITIT
     HADDAGSIAD GRNRVTGRIA DSSFIGVSTQ FVVNSPAGAE LQVFEQNVER DTRLVPGAEV
     VLHWNPAHTF GLDAAQDIDA GVETVEDAA
//
DBGET integrated database retrieval system