ID A0A2P4UJ12_9ACTN Unreviewed; 3480 AA.
AC A0A2P4UJ12;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE SubName: Full=Linear gramicidin synthase subunit B {ECO:0000313|EMBL:POM24998.1};
GN Name=lgrB_1 {ECO:0000313|EMBL:POM24998.1};
GN ORFNames=BTM25_36390 {ECO:0000313|EMBL:POM24998.1};
OS Actinomadura rubteroloni.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Thermomonosporaceae; Actinomadura.
OX NCBI_TaxID=1926885 {ECO:0000313|EMBL:POM24998.1, ECO:0000313|Proteomes:UP000242367};
RN [1] {ECO:0000313|EMBL:POM24998.1, ECO:0000313|Proteomes:UP000242367}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=5-2 {ECO:0000313|EMBL:POM24998.1,
RC ECO:0000313|Proteomes:UP000242367};
RX PubMed=28463423; DOI=10.1002/chem.201701005;
RA Guo H., Benndorf R., Leichnitz D., Klassen J.L., Vollmers J., Gorls H.,
RA Steinacker M., Weigel C., Dahse H.M., Kaster A.K., de Beer Z.W.,
RA Poulsen M., Beemelmanns C.;
RT "Isolation, Biosynthesis and Chemical Modifications of Rubterolones A-F:
RT Rare Tropolone Alkaloids from Actinomadura sp. 5-2.";
RL Chemistry 23:9338-9345(2017).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:POM24998.1}.
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DR EMBL; MTBP01000002; POM24998.1; -; Genomic_DNA.
DR Proteomes; UP000242367; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 2.
DR CDD; cd12117; A_NRPS_Srf_like; 1.
DR CDD; cd19543; DCL_NRPS; 1.
DR CDD; cd19540; LCL_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 6.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 3.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 4.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 1.
DR NCBIfam; TIGR01720; NRPS-para261; 2.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 5.
DR Pfam; PF13193; AMP-binding_C; 3.
DR Pfam; PF00668; Condensation; 5.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 8.
DR PROSITE; PS00455; AMP_BINDING; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000242367}.
FT DOMAIN 476..550
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1899..1974
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2966..3040
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 2050..2084
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2057..2083
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3480 AA; 363774 MW; 0CB3F93FAB1EB999 CRC64;
MLEGAPPAAT LVALVADRAR CAPDAPAVVD DDRTLTYAAL DAASGRLSGG LAARGVRRGD
RVGVVLDRSA DLVAVLLGVM RAGAAYVPVD PAWPAARRER VLADVACTIT SADLSGAGDD
PDLGPDDVAY VMYTSGSTGE PKGVAVTQGA LAELATAACW GDIGRGRVLF HAPHAFDASV
LELWVPLANG GTVVVAPDGP IDAERLRRVI RAHDVTAVHL TAGLFGALAA EDTGVLAGLA
DVLTGGDVVA PGAVAAVRRA FPELTVRHLY GPTEATLCAT THRIGPGDAA PDALPIGAPR
DGAGVHLLDD RLRPVPDGTV GELYIAGPCL ARGYDGRPAL TAERFVANPF GTGRLYRTGD
LARRSDGLLY FAGRADEQVK IRGFRVEPGE IEAVLAAHPD VERVAVVARG ERARLVAYPV
PASVDAAALT AYARERLPEY MVPAAVVPLD ALPLTRNGKL DRAALPEPSG PAAGRAPATP
AEETFCGLFA ALLGAAEVGA DTSFFALGGD SILSMSLVAR ARRAGYAVTT RQVFRLRTPA
ALAAGATVLD GAAEHDTATG EAPPTPVLLE LMERAGDLAH APGFYQSALV TVPSGLDLDV
LRGALHALAA HHGVLRARTA PSGGLLVPDG PDAEISVRRA DPAADVAACV DPRAGAMIDA
VWFEGDPGRL LLTIAHVAVD GVSWRVLLPD LAEAYEALAA GRAPALEPVP TAFPTWARSL
AAEAARRRPE LPEWKRILAG PDHAPVAAPD PAIDRYAGTG KITVTVPART AAALLADVPA
AFHAGVDEVL LTALAAALAP GGPLLVDVEG HGRADAVSRT VGWFTEIHPV RLDLGGLDPA
DVRAGHAAAG RALKRVKEQV RAVPGDGLGF GLLRHLEAAA ELAALPRPRI GFNYLGRFGG
TGAWRPAGTH WFAEGIDGRV PVMHALEAEA LVQDDALTLT LSYVRRLVTD EAAEQIASGW
TAALDGLVAH TARRGGGHTP ADFPLVALDQ RRIEELEAAV PGLADVLPLS PLQEGLLFHA
IYDDDATDVY VEQLAVDLAG PLDSGALRAA WQTVLDRHAS LRAGFRETAD RPVQVVAGRV
AVPWREAVAD AAGADRLADE ERARRFDVTR PPLLRVLLVR IGDAWRMTVT MHHIVMDGWS
LPVLLRELWT AYRGEPLGPV TPYRDYLEWL ARQDAVAARE AWRDALAGID EPTLVAPGRQ
DTPGLSGKVH FRPDDRLTAA LDALARTRGL TRGTILQGVW GLLVGKLTGR GDAVFGLTVA
GRPAELPGMA DMLGLFINTV PAVVRLDPAR PFADLLADLQ DRQAALADHQ HLGLTEIQRA
AGSGAVFDTL LTLENYAGVR AESGPPEVTG TRVREDTHYP LGLSMPPGGG LRISYRPDAF
DHDFALALAG RVLRVLEQVA ADPDVPAGRI EIVDGGERRR LLAEWNATDR ALPARTLPDL
FRAQAARTPD APAVAGAWTY AELDAVSDRV AGALAARGVR RGDLVGVLME RSPDLVAVLL
GIVKAGAAYV PADTGWPSAR VDAVLGGTKL VLTERPSGDA VPPVPVGPDD AAYVMFTSGS
TGTPKGVVAA HGAVAALAAD SCWSAAARGR VLLHAPHAFD ASTYELWVPL VHGGTVAVGP
PGRVDAAALE RLIGAHALTA VHVTAGLFGV LADETPGCLS GVAEVLTGGD VVASGAVARV
LEACPDVTVR HLYGPTETTL CATTHGLEPG GVVPAVLPIG RPRDNTRVYV LDPFLRPVPA
GVTGELYIAG LGVARGYLDR PALTAERFVA DPFGGGRMYR TGDRARWTRD GVLVFGGRAD
AQVKIRGYRV EPGEVEAVLA GHPAVRRAVV VVRDAKLYGY VVADAPGAAL RDFAAERLPE
YMVPAAVTVL DALPVTANGK VDRAALPVPG ERPGRGGRGP ATPVEAVLCG LFAEILGLDG
VGADDSFFDL GGDSLSAMRL TARIRAALGL EIGIGELFTA PTVAEVAELA GRATGGTRAA
VTRRERPEIL PPSFAQRRMW FLNRLEQTQD DVDAVYNLPL ALRMTGELDV AALEAALGDV
ADRHESLRTI FPDQPTPTDL PGFPDQPTPT DLPGFPDQPT PTDLPGFPET DDGPRQLVLP
SRPVLTVRSV PDLDAAVRDE GGRRFALAAE PPLRATLFVQ GPDEHVLLLV AHHIAVDGWS
MGVLGRDLGT AYAARLAGRA PEWEPLPVQY ADYALWQRDV LDDAVLDAQL AHWRRVLAGA
PAELALPTDR PRPAASTLRG GSVPLRVGAD THAALTGLAR DRRATMFMVA HAALAALLTR
MGAGDDLPIG TAIAGRGDPA LDGLIGFFVN TLVLRTDLSG NPTFAQLLDR VRDADLAAYA
HQDLPFERLV EDLNPERSLA RNPLFQVMLS VENVPREAGA WELPGLDVRA LPRRTDEPVE
FDLSVTLTER HDAGAPAGFD GLFQYAADLF DAATAEGLAR RLVAVLDQAA ADPHRPIGEL
AVLDAAERAR ILTGWNATDA TVSPATWPEL FAAHVARTPD AVAVRSGGDA LTYAELDDRS
ARLAGHLRSL GVGPETVVAL CLPRGVDWPV AQLAVWRAGG AFVPLDPEHP AERLAHIVAD
SGAALVIGTG DTLGPFDAPT TTPDVLGPPL RADVRPGQLA YVIYTSGSTG RPKGVAIEHR
GLVNLATALR PALGVAPGTV ALQFASFGFD AAVLDVAVVL GGGGTLAVAT AEERAEPDAL
AAMIRSAGVS VASVVPSLLG VLDPDAVPGV RRWVLGAELL TADLAARWTA RSTVWNTYGP
TEGTVIATSG PVDAGITAED RPPAVGRPLT NTRLYVLDEK LRPVPPGVVG ELYLTGVGLA
RGYAGRPDLT AERFVACPFR PGGARMYRTG DRARWTAGGE LLFAGRADEQ VKIRGFRVEP
GEVASVLAAH PDVAQAAVVV QDGRLVAYTV GGGADALRAH VAARVPDYMV PSAFVALDAL
PLTVNGKLDR AALPVPSAAS AEDAREPATP AEELLCGLFA DVLGLERVGP DDSFFALGGD
SILSMALVAR ARRSGLVLGV REVFRRRTPA ALAAVATALT DPAAADVPAT GAVPLTPVMR
ELLAGPDAER AVQSAVLAVP ADLDAGRLAD ALRAVIDHHD VLRARLEGDA LVVLPSGAPF
ALGAGVALDG PLLGAALSPG RLELVAHHLV IDGVSWQILL PDLRAAYEGR PLEATGTSFR
HWARALADQA ADRVAELPDW IRLLDGPSLR ITDAPVTPGP ARETAATLDA ATTEALLTRV
TAAFHAGMDE ILLAALTAAL TETWRRRGRD TSGGLLVDVE RHGREPLAEG MDLSRTVGWF
TAVHPVRLDA GALDLAAVRA GGPDAGTLVK RVKEQVRAVP GDGLGFGLLR RVHPGTGPVL
AALPAPGIAF NYLGRTPAAA SGDWRRLGER AAGDVPVRHA LDVMARITDG PVLTLTLASP
PGALDPADAR EIADGWAAAL TGLAAHTGGG HTPSDFLLST LDQHEIDLLE ADLADEGGPR
//
