ID   A0A2P5CTS4_PARAD        Unreviewed;       487 AA.
AC   A0A2P5CTS4;
DT   23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT   23-MAY-2018, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   SubName: Full=tRNA guanosine-2'-O-methyltransferase, TRM {ECO:0000313|EMBL:PON64440.1};
GN   ORFNames=PanWU01x14_125090 {ECO:0000313|EMBL:PON64440.1};
OS   Parasponia andersonii (Sponia andersonii).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Cannabaceae; Parasponia.
OX   NCBI_TaxID=3476 {ECO:0000313|EMBL:PON64440.1, ECO:0000313|Proteomes:UP000237105};
RN   [1] {ECO:0000313|Proteomes:UP000237105}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. WU1-14 {ECO:0000313|Proteomes:UP000237105};
RA   Van Velzen R., Holmer R., Bu F., Rutten L., Van Zeijl A., Liu W.,
RA   Santuari L., Cao Q., Sharma T., Shen D., Roswanjaya Y., Wardhani T.,
RA   Kalhor M.S., Jansen J., Van den Hoogen J., Gungor B., Hartog M.,
RA   Hontelez J., Verver J., Yang W.-C., Schijlen E., Repin R., Schilthuizen M.,
RA   Schranz E., Heidstra R., Miyata K., Fedorova E., Kohlen W., Bisseling T.,
RA   Smit S., Geurts R.;
RT   "Parallel loss of symbiosis genes in relatives of nitrogen-fixing non-
RT   legume Parasponia.";
RL   Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalytic subunit of an S-adenosyl-L-methionine-dependent
CC       tRNA methyltransferase complex that mediates the methylation of the
CC       guanosine nucleotide at position 10 (m2G10) in tRNAs.
CC       {ECO:0000256|ARBA:ARBA00002425}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PON64440.1}.
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DR   EMBL; JXTB01000096; PON64440.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2P5CTS4; -.
DR   STRING; 3476.A0A2P5CTS4; -.
DR   OrthoDB; 5488599at2759; -.
DR   Proteomes; UP000237105; Unassembled WGS sequence.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR000241; RlmKL-like_Mtase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR016691; tRNA_mtfrase_TRM11.
DR   PANTHER; PTHR13370; RNA METHYLASE-RELATED; 1.
DR   PANTHER; PTHR13370:SF3; TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG; 1.
DR   Pfam; PF01170; UPF0020; 1.
DR   PIRSF; PIRSF017259; tRNA_mtfrase_TRM11; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
DR   PROSITE; PS51627; SAM_MT_TRM11; 1.
PE   4: Predicted;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW   ECO:0000313|EMBL:PON64440.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000237105};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022555};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:PON64440.1};
KW   tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT   DOMAIN          202..324
FT                   /note="UPF0020"
FT                   /evidence="ECO:0000259|Pfam:PF01170"
FT   REGION          463..487
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   487 AA;  55141 MW;  23DAA7BD63A0BA6D CRC64;
     MWYLCVFYHR LLDYRKSEVE SLAQLFGAFD PEGLDQQNPK GYCSLEWRLP QHHHPDSPFH
     FVNLPSEDVA RKIANRSILV KGMYELWGEG DSYKELEESI KSYPDERKLP YLESGSTFKI
     NVDTFGKVLS LQDQNQRIQG LSYIPFNGRV NLKNPDHKFW LMETDDYGSN NGLPPIVQRR
     IFFGREIGGA DRKLIPTYQL KSRTYLGPTA MDAEMAFLMA NQALALPGKL VYDPFVGTGS
     ILVAAAHFGA MTMGADIDIR VVRDGRGPDC NVWSNFKQYG LPMPIALLRA DNNLPPWRPG
     LKEVFDAIIS DPPYGVRAGG RKSGGRKLLK GAVSPYVVPD DKRTDHIPST APYSLVECVH
     DLLDLAARLI VMGGRLVFFY PVLREDDYVE NHFPEHQCFK LIASSEQILS SRYSRVLLTM
     VKISPYTEEI ADAARIKHME FRENHLKWLE NGNLHSVVFS PADSNSNASG EGKLSKESKP
     KYRGKYV
//