ID A0A2P6NPV2_9EUKA Unreviewed; 335 AA.
AC A0A2P6NPV2;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=SAM-dependent MTase RsmB/NOP-type domain-containing protein {ECO:0000259|PROSITE:PS51686};
GN ORFNames=PROFUN_06101 {ECO:0000313|EMBL:PRP85979.1};
OS Planoprotostelium fungivorum.
OC Eukaryota; Amoebozoa; Evosea; Variosea; Cavosteliida; Cavosteliaceae;
OC Planoprotostelium.
OX NCBI_TaxID=1890364 {ECO:0000313|EMBL:PRP85979.1, ECO:0000313|Proteomes:UP000241769};
RN [1] {ECO:0000313|EMBL:PRP85979.1, ECO:0000313|Proteomes:UP000241769}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Jena {ECO:0000313|EMBL:PRP85979.1,
RC ECO:0000313|Proteomes:UP000241769};
RX PubMed=29378020; DOI=10.1093/gbe/evy011;
RA Hillmann F., Forbes G., Novohradska S., Ferling I., Riege K., Groth M.,
RA Westermann M., Marz M., Spaller T., Winckler T., Schaap P., Glockner G.;
RT "Multiple Roots of Fruiting Body Formation in Amoebozoa.";
RL Genome Biol. Evol. 10:591-606(2018).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRP85979.1}.
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DR EMBL; MDYQ01000037; PRP85979.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2P6NPV2; -.
DR STRING; 1890364.A0A2P6NPV2; -.
DR InParanoid; A0A2P6NPV2; -.
DR Proteomes; UP000241769; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22808; NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR22808:SF3; SAM_MT_RSMB_NOP DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000241769};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 56..329
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 273
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 148..154
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 173
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 204
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 220
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 335 AA; 38462 MW; 616430F498A90119 CRC64;
MLINRVALLY NTTHAPIPVT HRCRWYTTAV DGQTTKKNKP LSKNKEKALA KERLQLEKAT
NAFNTLYTDM YGAERYSNEE QMNTTVKRLQ KLEKVEHINT LAYTSSERFP QPPKDHRGLS
IYYPLDAASL LVVDALRLEP DHQVLDMCAA PGGKTVGIMQ HLSNPSASLT SNEISPDRLR
RLKQVLRDYL GDEYRQRVTV TRRDAMHWRE EEKYHRVLLD APCSAERHLL RDADELSKWT
PKRTSTLYKE QVVMLKSALR AVRVGGLVVY STCSISNKEN DAVIERVLDK SKVIKKEWKV
GEQTTHGWAV LPDRSDGWGP MYFSVLERLP DDPSQ
//
