ID A0A2P6QLV2_ROSCH Unreviewed; 3777 AA.
AC A0A2P6QLV2;
DT 23-MAY-2018, integrated into UniProtKB/TrEMBL.
DT 23-MAY-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=RchiOBHm_Chr5g0077001 {ECO:0000313|EMBL:PRQ35163.1};
OS Rosa chinensis (China rose).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Rosoideae; Rosoideae incertae sedis;
OC Rosa.
OX NCBI_TaxID=74649 {ECO:0000313|EMBL:PRQ35163.1, ECO:0000313|Proteomes:UP000238479};
RN [1] {ECO:0000313|EMBL:PRQ35163.1, ECO:0000313|Proteomes:UP000238479}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Old Blush {ECO:0000313|Proteomes:UP000238479};
RA Bendahmane M.;
RT "The Rosa genome provides new insights in the design of modern roses.";
RL Nat. Genet. 0:0-0(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PRQ35163.1}.
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DR EMBL; PDCK01000043; PRQ35163.1; -; Genomic_DNA.
DR STRING; 74649.A0A2P6QLV2; -.
DR EnsemblPlants; PRQ35163; PRQ35163; RchiOBHm_Chr5g0077001.
DR Gramene; PRQ35163; PRQ35163; RchiOBHm_Chr5g0077001.
DR OMA; ADEMKYG; -.
DR OrthoDB; 1214833at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000238479; Chromosome 5.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14327; UBA_atUPL1_2_like; 1.
DR Gene3D; 6.10.250.1630; -; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR003903; UIM_dom.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00627; UBA; 1.
DR Pfam; PF14377; UBM; 3.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50030; UBA; 1.
DR PROSITE; PS50330; UIM; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:PRQ35163.1};
KW Ligase {ECO:0000313|EMBL:PRQ35163.1};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000238479};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:PRQ35163.1};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1296..1337
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 3436..3777
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 462..481
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 699..737
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 921..941
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1725..1754
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2034..2110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2155..2225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2435..2489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2523..2550
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2695..2718
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2987..3006
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3053..3106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 710..726
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2040..2054
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2055..2106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2161..2193
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2208..2225
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2437..2455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2462..2476
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2523..2540
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3059..3085
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3092..3106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3744
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3777 AA; 414302 MW; 2B7BBE551BA8E551 CRC64;
MKLKRRRAVE VPPKIRSFIN SVTAVPLENI EEPLKGFVWE YDKGDFHHWV DLFNHFDSFF
EKHIKSRKDL QVEDNFLDSD PPFPREALLQ VLRVIRIILE NCTNKHFYSS YEQHLSSLLA
CTDADVVEAC LQTLAAFLKK TVGKYSIRDA SLNSKLFALA QGWGGKEEGL GLIACAVQDG
CDPIAYELGC TLHFEFYALK DASELPTTEQ QTQGLQIIHL PNINTHPETD LELLSKLIAE
YKVPSSLRFA LLTRLRFARA FGSLATRQQY ACIRLYAFIV LVQANSDGDD LVSFFNTEPE
FVNELVSLLS LEDVVPEKIR ILCLLSLVAL SQDRARQPNV LTAVTSGGHR GILSSLMQKA
IDSVISDTSK WSVVFAEALL SLVTVLVSSS SGCSAMREAG FIPTLLPLLK DTNPQHLHLV
STSVHILEAF MDYSNPAAAL FRDLGGLDDT ISRLQLEVSH VENGPKQQDE DSSTSGSAQV
VAGTSTEIDS MQPLYSEPLV SYHRRLLMKA LLRAISLGTY APGNTARVYG SEESLLPQCL
CIIFKRAKDF GGGVFSLAAS VMSDLIHKDP TCFPVLDEAG LPSTFLDAIM DGVLCSTEAI
TCIPQCLDAL CLNNNGLQAV KDRNALRCFV KIFTSRTYLR ALTSDTPVSL SSGLDELMRH
ASSLRGPGVD MLIEILNVIS KIGHGGDSFL MSTELLSSST PVPMETDGEE RNVVMSDDRE
SSKMDSSEQG TETSSDSLVG NAEVLLPDCV SNVARLLETI LQNGDTCRIF VEKKGIEAVL
QLFTLPLMPL SVSVGQSISI AFKNFSPQHS ASLARAVCSF LREHLKSTNE LLVSVGGTQL
SLVESAKQTK VLKHLSSLEA ILSLSNVLLK GTTTVVSELG AADADVLKDL GSTYREILWQ
ISLCNDVKSD EKINAEQEQD IAEAAPSNAS GRESDDDANT PMVRYMNPVS IRNQPFWGGE
REFLSVVRSG EGLHRRSTRH GITRMRGGRT GRHLEALHID SESSSTVSEA TTSQDLKKKS
PDVLVTEILN KLASTLRSFF TALVKGFTSP NRRRVDSGSL SLASKTLGTA LAKVYLEALI
FPGHSTSAGL DTSLSVKCRY LGKVVDDMMA LTFDSRRRTC YTATINNFYV HGTFKELLTT
FEATSQLLWT LPYCMPTSGI DQEKTGEGSK LSHSSWLLET LQSYCRVLEY FVNSSLLLST
TSASQAQLLV QPVAVGLSIG LFPVPREPEV FVRMLQSQVL DVILPIWNHP MFPNCSPGFV
ASIVSLVMHV YSGVGDVKQN RSGIAGTANQ RFMPPPLDEA TITTIMEMGF SRVRAEEALR
RVETNSVEMA MEWLCSHAED PVQEDDELAR ALALSLGNSS ETSKADNVDK SVDVLAEESC
VKAPPVDDIL AASVKLFQSS DTMAFPLTDL LVTLSNRNKG DDRPKVASYL IQQLKLCPLD
FSKDTSSLSM LSHVIALLLS EDGSTREIAA QNGIVSAAVD ILMNFKAKDE SGNELLVPKC
ISALLLILDN MLQSRPRISE NIEETQTGSL SELSGERTSL SIPDAVTEKK QDTDAQEKDS
GTGFEKILGK STGYLTMEES QKVLAVACDL IKQHVPAMIM QAVLQLCARL TKTHALALQF
LEHGGLAALF GLPRSCFFPG YDTVASAIVR HLLEDPQTLQ TAMELEIRQT LSGNRHGART
SARTFLTSMA PVISRDPVVF MKAAAAVCQL ETSAGRTFIV LMKEKEKEKD KPKASGGEVG
LSSNESIRIP ENKVHDGSGK CLKGHKKIPA NLTQVIDQLL EIVLKYYFPK SQEDCQNNLS
SMEVDEPATK VKGKSKVDET RKVESESERS AGLAKVTFVL KLLSDILLMY VHAVGVILKR
DLELAQLRVA NQLEGPGHGG ILHHVIHRLL PLTIDKSAGP DEWRDKLSEK ASWFLVVLCG
RSGEGRRRVI NELVKALSSF SNIDGNSSKS ILLPDKKVYA FVDLVYSILS KNSSSSNLPG
TGFSPDIAKS MIDGGMIQCL TSILQVIDLD HPDAPKTVNL ILKALESLTR AANASEQYFK
SDETNKKKSM VLNRRSDDQV TTPADDTLGH NQNTSSEQDV RDAVPTEQQD QGTSQSEGNP
DATPNQSGEH DMRIEVEGPL ASNQPMELGM DFMREEMEEG NVLHNNDQIE MTFRVEGRAD
DDMGDEDDDM GDDGEDDEDD DDGEDEDEDI AEDGGGMMSL ADTDVEDHDD TGLGDDYNDE
MIDEDDDDFH ENRVIEVRWR EALDGLDHLH VLGQPGAASG LIDVAAEPFE GVNVDDLFGL
RRPLGFDRRR QTSRSSFERS VAEANGFQHP LLVRPPQSGD LVSMWSAGGN SSRDLEALSS
GSFDVAHFYM FDAPVLPYDH VPSNLFGDRL GGAAPPPLTD YSVGMDSLQL SGRRGPGDGR
WTDDGQPQAG AHAAAIAQAV EEQFISQLRS LAPVDTPVEP HSQNSVVQEK QPDVPPSTDG
QVVVDRGNTT SQQVEDQHQD RGVEVTHQDI SRPEGIPRQE QVNQESFVED AGGCLQEPEP
MSIQAPSLDS TRNDNMDIGE GNGTAEQEGS MPEFVNSSAS TRVDLQQEGG SEVPSNVNDA
TVEAMGQDGS SGNQAGDMPA NFGFNVSNSG DSHTSLVPEN VDVDMNCIDE VNQTGHPMPA
FENGTDEPSS QNTLVAPEAN QAEQVTLNNE APGANAIDPT FLEALPEELR AEVLASQQAQ
SVQPPPYAPP SADDIDPEFL AALPPDIQAE VLAQQRAQRV AQQAEGQPVD MDNASIIATF
PADLREEVLL TSSEAVLSAL PSPLLAEAQM LRDRAMSHYQ ARSLFGSSHR LNNRRNGLGF
DRHTVMDRGV GVTIGRRAVS SITDSLKGKE NEGEPLLDAN SLKALIRLLR LAQPLGKGLL
QRLFLILCTH SVTRAILVRL LLDLIKPEAE GSVSGLATIN SQRLYGCHSN VVYGRSQLLD
GLPPLVLRRI LEILTYLATN HSAVANMLFY FDFSGVPQSL SPLNMDTKKD KGKEKIGEGG
FSSNPVNTQD GDVPLILFLK LLNRPHFLRS TAHLEQVMDL LQVVVDNSAS KLEVHSPSER
VDGNSQNLSI SETSGDGQNG RPVEPESQQE VKPDGVGSST SDANKSTDTH SIFLKLPESD
LHNLCSLLGR EGLSDKVYML SSEVLKKLAS VAVTHRKFFI SELSELAHGL SASAVSELVT
LRNTQMLGLS AGSMAGSAIL RVLQSLCSLT SPGTNENSVL ENDAEQEEHA TMWKLNIALE
PLWQELSDCI SATETQLGQS SFCPTMSTIN IGDHVQGSSS SSPLPPGTQR LLPFMEAFFV
LCQKLQANHS ITLQDQANVT AREVKESAGN SDPSGTKFYS CGDSQRKLDG AVTFTRFAEK
HRRLLNAFIR QNPGLLEKSL TMMLKAPRLI DFDNKRAYFR SRIRQQHEQH LSGPLRISVR
RAYVLEDSYN QLRMRPNQDM KGRLNVQFQG EEGIDAGGLT REWYQLLSRV IFDKGALLFT
TVGNNATFQP NPNSVYQTEH LSYFKFVGRV VAKAVFDGQL LDVYFTRSFY KHILGVKVTY
HDIEAVDPDY YKNLKWMLEN DVSDIPDLTF SMDADEEKHI LYEKNQVTDY ELKPGGRNIR
VTEETKHEYV DLVADHILTN AIRPQINSFL EGFNELVPRE LISIFNDKEL ELLISGLPEI
DLDDLKANTE YTGYTAASSV VQWFWEVVKC FNKEDMARLL QFVTGTSKVP LEGFKALQGI
SGPQRFQIHK AYGAPDRLPS AHTCFNQLDL PEYTSKDQLH ERLMLAIHEG SEGFGFG
//
